TBLASTN 2.2.21 [Jun-14-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= DPS_ECOLI Fe-binding and storage protein (167 letters) Database: xc 460 sequences; 5,103,728 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value AE012409 AE008922 Xanthomonas campestris pv. campestris str. ATC... 58 6e-10 >AE012409 AE008922 Xanthomonas campestris pv. campestris str. ATCC 33913, section 317 of 460 of the complete genome. Length = 9982 Score = 57.8 bits (138), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 42/149 (28%), Positives = 71/149 (47%), Gaps = 5/149 (3%) Frame = +2 Query: 21 VSDSEKKATVELLNRQVIQFIDLSLITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTM 80 + D+++K + L R + L L T HWN+ G+ F ++H M + T LD + Sbjct: 7268 IKDADRKQISDGLARYMADAFTLYLKTHNFHWNVTGSMFNSLHTMFETQYTEQWGALDEV 7447 Query: 81 AERAVQLGGVALGTTQVINSKTPLKSYP--LDIHNVQDHLKELADRYAIVANDVRKAIG- 137 AER LG A G+ + + T + P D + ++ +++L V RK +G Sbjct: 7448 AERIRALGYNAPGSYREFVALTSIPEEPGLSDSADWREMVRQLVSGNEAVCRTARKVLGT 7627 Query: 138 --EAKDDDTADILTAASRDLDKFLWFIES 164 +A DD T D+LT + +K+ W + S Sbjct: 7628 ADDAGDDPTVDLLTQRLQTHEKYAWMLRS 7714 Database: xc Posted date: Nov 2, 2009 10:10 PM Number of letters in database: 5,103,728 Number of sequences in database: 460 Lambda K H 0.318 0.132 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 460 Number of Hits to DB: 597,545 Number of extensions: 6445 Number of successful extensions: 67 Number of sequences better than 1.0e-03: 1 Number of HSP's gapped: 67 Number of HSP's successfully gapped: 1 Length of query: 167 Length of database: 1,701,242 Length adjustment: 80 Effective length of query: 87 Effective length of database: 1,664,442 Effective search space: 144806454 Effective search space used: 144806454 Neighboring words threshold: 13 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 43 (21.2 bits)