FastQCFastQC Report
Вт 25 дек 2018
chr6.1_tri.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamechr6.1_tri.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53819
Sequences flagged as poor quality0
Sequence length50-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATG578610.750850071536075No Hit
CTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAG32806.094501941693454No Hit
GGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGA26734.96664746650811No Hit
GGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGC23904.440810866051023No Hit
CTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTG22614.201118564075884No Hit
CAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAAT17413.234917036734239No Hit
GCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCA14962.7796874709675023No Hit
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCAT14442.6830673182333378No Hit
CCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATT13152.443375016258199No Hit
GCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAA11092.0606105650420856No Hit
GGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAG9251.7187238707519648No Hit
CAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCT8871.6481168360616139No Hit
CATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATGCC7711.4325795722700163No Hit
CCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAG7211.3396755792563964No Hit
ATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATGCCA6631.2319069473605975No Hit
CCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT6611.2281907876400529No Hit
TCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT6001.1148479161634366No Hit
ATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTA5781.073970159237444No Hit
GTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACATAGTA5681.0553893606347202No Hit
CCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATGC5380.9996469648265482No Hit
GTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGTC5340.9922146453854588No Hit
CCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATA4700.8732975343280254No Hit
GGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA4550.8454263364239396No Hit
GGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGT4430.8231293781006708No Hit
GCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT4320.8026904996376745No Hit
CCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAA4300.7989743399171295No Hit
TTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA4290.7971162600568573No Hit
CTGGCATTGCCATCCTTACGGGTGACTTTCCATCCCTTGAACCAAGGCATG4170.7748193017335885No Hit
TGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGG3780.7023541871829652No Hit
GCATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCT3680.6837733885802412No Hit
TTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTC3490.6484698712350656No Hit
CGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGC3480.6466117913747933No Hit
GGAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCT3390.6298890726323417No Hit
CTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCT3360.6243148330515246No Hit
CATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAG3310.6150244337501626No Hit
GGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCC3220.598301715007711No Hit
CACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCT3000.5574239580817183No Hit
GTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATG2940.546275478920084No Hit
GTGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGA2930.5444173990598116No Hit
GCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCA2870.5332689198981772No Hit
CTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA2790.5184042810159981No Hit
GTCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGC2680.49796540255300176No Hit
GGACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTTCCA2670.4961073226927294No Hit
GTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA2610.484958843531095No Hit
TGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGT2360.43850684702428516No Hit
ATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGT2300.42735836786265075No Hit
GTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTC2250.41806796856128875No Hit
GCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTT2230.41435180884074396No Hit
CTTCAAATTCACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCT2210.4106356491201992No Hit
GCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGA2180.40506140953938197No Hit
GCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATT2080.3864806109366581No Hit
GGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG2010.3734740519147513No Hit
CCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGAT1980.3678998123339341No Hit
CTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGC1970.36604173247366173No Hit
CACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCC1920.3567513331722998No Hit
CTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGCTC1870.3474609338709378No Hit
GAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTT1870.3474609338709378No Hit
GTCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCAT1850.343744774150393No Hit
GTCTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGC1740.32330589568739665No Hit
ACCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT1710.31773165610657944No Hit
CAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGTCATT1700.3158735762463071No Hit
AGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG1690.31401549638603465No Hit
ATCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA1660.3084412568052175No Hit
GCAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCCCTGTAATCATG1630.3028670172244003No Hit
GCCTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGG1560.28986045820249356No Hit
CGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAG1540.2861442984819488No Hit
TGGACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTTCC1460.27127965959976963No Hit
GACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTTCCAC1440.26756349987922484No Hit
TGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTAT1420.26384734015868005No Hit
GTCAGCCTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCC1320.2452665415559561No Hit
CTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTC1250.23225998253404934No Hit
CCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTG1230.22854382281350455No Hit
CGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG1210.2248276630929597No Hit
GCTTCAAATTCACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCC1190.22111150337241495No Hit
GCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGC1170.21739534365187016No Hit
GTGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAG1160.21553726379159774No Hit
GCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATC1100.2043887846299634No Hit
GTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCC1050.1950983853286014No Hit
TTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACATAGTACT1050.1950983853286014No Hit
GTTTGAGAACACCAGTCTCCACTCGGCCAACAGGAACAGTACCAATACCAC1000.18580798602723944No Hit
GTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAG990.18394990616696705No Hit
CATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTT980.18209182630669465No Hit
ACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCCC930.1728014270053327No Hit
GATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTG890.1653691075642431No Hit
CAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCCCTGTAATCATGT890.1653691075642431No Hit
TTCAAATTCACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCTG860.15979486798342593No Hit
GGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAG830.15422062840260875No Hit
TCACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCC810.15050446868206396No Hit
TTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCA810.15050446868206396No Hit
ATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGCTCTCAAC770.1430721492409744No Hit
GTCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA760.14121406938070197No Hit
CATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACATA740.13749790966015718No Hit
CACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGTCATTTTTGCTGTC740.13749790966015718No Hit
CGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATG730.13563982979988481No Hit
CGGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAG690.1282075103587952No Hit
GGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG680.12634943049852282No Hit
CGCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATG650.12077519091770565No Hit
GTTGGACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTT650.12077519091770565No Hit
AGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTT640.11891711105743324No Hit
CTGTCTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAA640.11891711105743324No Hit
CCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGAT640.11891711105743324No Hit
CCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGT640.11891711105743324No Hit
CGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGA610.11334287147661606No Hit
ATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACA610.11334287147661606No Hit
TGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG590.10962671175607129No Hit
CCTTGAACCAAGGCATGTTAGCACTTGGCTCCAGCATGTTGTCACCATTCC580.10776863189579887No Hit
CGGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTG570.10591055203552648No Hit
CCTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGG540.10033631245470931No Hit
CTTGCATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGC540.10033631245470931No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCCA700.045.61922545
GTCATAG302.0917705E-645.1090431
GTGGCAT302.0917705E-645.1090431
GCCCATC551.8189894E-1245.109041
CTCCCAT253.786626E-545.109041
GTCTGGA351.1609336E-745.109041
CTGGCAT453.619789E-1045.109041
GTCCCAT351.1609336E-745.109041
CCCCATC700.045.109041
TGGCATT453.7107384E-1044.9832732
TGGCATA302.1324795E-644.9832732
TCTGGAT351.18678145E-744.983272
GGCATTG501.0440999E-940.4849433
CATTGCC501.0440999E-940.4849435
CATCCTT501.0440999E-940.48494311
ATTGCCA501.0440999E-940.4849436
TGCCATC501.0440999E-940.4849438
CCATCCT501.0440999E-940.48494310
GCCATCC501.0440999E-940.4849439
GCATTGC501.0440999E-940.4849434
Produced by FastQC (version 0.11.2)