Выравнивание последовательностей
Практикум 11: Построение парных выравниваний. Поиск по сходству
Задание 1. Поиск гомологов белка в базе данных NCBI
В задании требовалось собрать выборку белков, гомологичных Metallo-beta-lactamase type 2 [Klebsiella pneumoniae].
Для нахождения белков в базе данных Refseq использовалась программа protein BLAST на сайте NCBI.
Мы ограничили поиск таксоном Gammaproteobacteria (taxid:1236), чтобы уменьшить количество находок. Настройки поиска можно посмотреть
здесь .
Cравнение белков
Параметр/Находка
Лучшая
Средняя
Худшая
Sequence ID
WP_063860854.1
WP_032492622.1
WP_025733014.1
Название белка
subclass B1 metallo-beta-lactamase NDM-11
subclass B1 metallo-beta-lactamase IMP-34
adenosylcobinamide kinase/adenosylcobinamide phosphate guanyltransferase
Организм
Escherichia coli
Klebsiella oxytoca
Carnimonas nigrificans
Bit Score
554
133
35.8
% идентичных остатков
99%
34%
34%
% сходных остатков
100%
52%
50%
E-value
0.0
2e-35
4.8
Выравнивание из BLAST
WP_063860854.1_with_our.html
WP_032492622.1_with_our.html
WP_025733014.1_with_our.html
Всего было обнаружено 365 находок, из них 179 оказались гомологичны нашему белку по всей длине
(query cover > 80% ).
Если руководствоваться критерием (query cover > 70%) & (E-value < 1e-3) , обнаруживается 233 гомолога.
Задание 2. Множественное выравнивание выборки
Нами было отобрано 25 не слишком сходных последовательностей (см.
таблицу ). Эти последовательности были скачаны в формате fasta,
к ним добавлена последовательность белка Metallo-beta-lactamase type 2 (см.
файл ).
С помощью программы MUSCLE было построено множественное выравнивание этих последовательностей (см.
файл ).
Launch in Jalview
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- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M R W L L L I - - - - F L S L C - - - - - - - - - G P A W A N - - - - - - - T D Y - A L K P R Q I A E G - T W L L E G S T E N F A K A N G - G N I V N T A F I V T D A G - V V V I D T G P S K R Y G E A L R Q A I A A T T D K P V V Q V L L T H H H P D H V L G N Q A F S A V P I G A L A G - - T T D L L R Q Q - - - - - - - G D A M A E N M - - Y - - - - R L V G D W M R G T E V - - V L P T Q V L E P G V L K V G N H S L R L L Q L A G H T G A D L A I L D E T T G V L F A G D L V F Y E R A - - L T T P N S P G L D V W L N D L D T L Q A - - - - - - - - - - - - - L P W K Q I V P G H G P V - A T D A K P F A Q M R D Y L G W L D Q L M R D G A V R G D D M A E M I R S P I P E R F A G I S L S R Y E L I R S V S H L Y P R Y E R A G M K R V D A P A Q - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M R W I L L V C L S L S L S L S - - - - - - - - - L P A R A E - - - - - - - L D Y - R L E P R Q I A E D - T W L L E G G T E N F S R D N G - G N I V N T G F I V T E A G - V L V I D T G P S K R Y G Q A L R E A I A R V T G K P V I Q V L L T H H H P D H V L G N Q A F S D V P I G A L A G - - T T E L L R Q Q - - - - - - - G D A M A E N L - - Y - - - - R L V G D W M R G T E V - - V L P N R T L E P G V L E I G G H R L R L L A L A G H T G A D L A I F D E R T G V L F A G D L V F Y Q R A - - L T T P N S P G L E V W L K D L D R L Q A - - - - - - - - - - - - - L P W R L V V P G H G P V - A S S A V P F A Q M R D Y L G W L H Q L L S D G A A R G D D M A E L I R A P I P A R F A A I S L T R Y E L I R S V S H L Y P R H E R E R M Q R V D T P - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 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E V R L Y Q I A D G - V W S - - - - H I A T Q S F D G - A V Y P S N G L I V R D G D E L L L I D T A W G A K N T A A L L A E I E K Q I G L P V T R A V S T H F H D D R V G G V D V L R A A G V A T Y A S P S T R R L A E V E - - - - - - - G S E I P T H S - - L - - - - E G L S S S G - D A V R - - F G P V E L F Y P G - - - - - - - - - - - - - - A A H S T D N L V V Y V P S A S V L Y G G C A I Y E L S R T S A G N V A D A D L A E W P T S I E R I Q Q - - - - - - - - - - - H Y P E A Q F V I P G H G L P - G G L - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L L K H T T N V V K A H T N R S V V E - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M L K V I S S L L V Y M T A S V M A V A S P L A H S G E P S G E Y P T V N E I P V G - E V R L Y Q I A D G - V W S - - - - H I A T Q S F D G - A V Y P S N G L I V R D G D E L L L I D T A W G A K N T A A L L A E I E K Q I G L P V T R A V S T H F H D D R V G G V D V L R A A G V A T Y A S P S T R R L A E A E - - - - - - - G N E I P T H S - - L - - - - E G L S S S G - D A V R - - F G P V E L F Y P G - - - - - - - - - - - - - - A A H S T D N L V V Y V P S A K V L Y G G C A V H E L S R T S A G N V A D A D L A E W P T S V E R I Q K - - - - - - - - - - - H Y P E A E V V I P G H G L P - G G L - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L L Q H T A N V V K A H K N R S V A E - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M L K V I S S L L V Y M T A S V M A V A S P L A H S G E P S G E Y P T V N E I P V G - E V R L Y Q I A D G - V W S - - - - H I A T Q S F D G - A V Y P S N G L I V R D G D E L L L I D T A W G A K N T A A L L A E I E K Q I G L P V T R A V S T H F H D D R V G G V D V L R A A G V A T Y A S P S T R R L A E A E - - - - - - - G N E I P T H S - - L - - - - E G L S S S G - D A V R - - F G P V E L F Y P G - - - - - - - - - - - - - - A A H S T D N L V V Y V P S A N V L Y G G C A V L E L S S T S A G N V A D A D L A E W P T S V E R I Q K - - - - - - - - - - - H Y P E A E V V I P G H G L P - G G L - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L L Q H T A N V V K A H K N R S V A E - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M L K V I S S L L V Y M T A S V M A V A S P L A H S G E P S G E Y P T V N E I P V G - E V R L Y Q I A D G - V W S - - - - H I A T Q S F D G - A V Y P S N G L I V R D G D E L L L I D T A W G A K N T A A L L A E I E K Q I G L P V T R A I S T H F H D D R V G G V D V L R A A G V A T Y A S P S T R R L A E A E - - - - - - - G N E I P T H S - - L - - - - E G L S S S G - D A V R - - F G P V E L F Y P G - - - - - - - - - - - - - - A A H S T D N L V V Y V P S A N V L Y G G C A V H E L S S T S A G N V A D A D L A E W P T S V E R I Q K - - - - - - - - - - - H Y P E A E V V I P G H G L P - G G L - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L L Q H T A N V V K A H K N R S V A E - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M R V T Q L L L L L F F A P L F I A P - - - - - - A C L A A E T Q D R N I I E L S A D L Q V Y P L T D D - V W V - - - - H R S W A D L A G - T R Y P A N G L L I N N D D T L V L I D T A W G N D S T A L L L E W V T V K F G K Y P D V A I L T H A H A D R I G G L P A L A E H H I P A Y V H P M T R E L A A S Q - - - - - - - - - - Y P E E Q - - L P E V I P G L E K H A - - S A R - - L E F L E A F Y P G - - - - - - - - - - - - - - P A H S P D N I V V W Y D S M K L L F G G C A V K S A D A G D I H Y V E G S S P E G W E A A I G N I Q G - - - - - - - - - - - R Y P E A T T V I P G H G A V - G G A - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Q L L D H T R K L A R Q G N Q P - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M R L F A L V I T L L I P L A V L A V - - - - - - - - - - E P V G K - - - - - P I R L S N D L V V Q P L T E H - V W L - - - - H R S W T E Y N G - Q R V S S N G V I I D D G K T L T L I D T A W G E A P T A T L L S W I E Q H F G K D P D Q A I L T H S H D D R M G G S R L L A E K H I R A Y V H P K T L S I V K A E - - - - - - P G K Y Y D P A S - H F - - - - P Q A L A F H D R R A R - - L G D I E L Y Y P G - - - - - - - - - - - - - - A A H S P D N I V I W Y G R D H L L V G G C A V K G R E S T N I G Y V A G S A P D H W A A A M D N L I Q - - - - - - - - - - - A F P Q A A M V V P G H G Q L - G D E - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Q L L F H T R A L A T Q A L E R R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - B B B B B B B B B B B B B B B B B B B B B B - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 1 0 0 0 0 0 1 0 0 0 1 0 1 0 0 0 - - - - - - - - - - 0 0 0 3 0 - - - - - - - 0 0 0 - 1 8 2 5 2 3 7 4 3 1 - 6 + 5 - - - - - - - - - - 0 0 1 - 0 0 1 0 0 1 2 7 5 3 1 3 5 3 0 7 5 7 9 5 1 1 0 1 2 3 2 7 3 1 9 3 3 2 6 2 2 2 - 3 4 1 6 2 2 5 9 5 9 5 3 * 3 * 4 5 7 * 5 2 2 7 2 4 2 2 5 3 3 6 5 2 0 0 5 3 4 + 6 2 3 6 - - - - - - - 0 0 0 2 2 2 2 4 - - 7 - - - - 0 0 1 0 0 0 0 - 0 0 0 3 - - 2 3 1 4 3 5 7 3 5 * - - - - - - - - - - - - - - 1 3 * 7 2 5 2 9 4 5 5 3 3 3 3 0 2 7 6 9 7 6 4 9 4 2 1 - - - - 7 3 5 5 4 3 6 4 2 3 1 8 1 2 5 7 3 2 8 3 4 - - - - - - - - - - - 0 1 3 3 4 3 5 9 7 5 5 * 3 1 6 - 2 5 1 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 4 7 5 2 2 5 1 1 6 5 2 3 2 1 0 0 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M R S R N W S R T L T E R S G G S G A V L V F M A C Y D C F F V Q S M P R A S K Q Q A R Y A V G R C L M L W S S N D V T Q Q G S R P K T K L C R T H M E L P N M M K G W S K L L L A L A A S L M A + A S P L A + S A S F W G E Y P T V N S + R L G + D L K L Y Q L A D G Y V + V L E G + H T S F Q + V E G N G V V P S N G L V V R D G K E V L L I D T A W T A D + T A + L L K W I K R V I G + P V L E + I S T H F H T D R + G G N D Y L K S A G I + T Y A S + L T R D L A K S D W A E I V A F G R V G A + H S P D L P L V L P G V + H W G D F T L R P N F G K V E V F Y P G V L + + G + H + L R L L + L P A H T P D N I V V Y F P + + K V L F G G C + V K E K + A T S L G N L S D A D L E A W P Q S I E R L Q A L K D T T K V Y C A H M Y P P A K + V V P G H G S P L G G P A + P F A Q M R D Y L G W L + Q L + + D G A + R G D D M A E + I R + P I P + R F A + I S L + R Y E L L D H T E E L A K A A P N S S + K E S + P S N + + K T L N P F L R C S D T G L K N A L Q N K L C D E I H D A L H C F T K L R Q Y K D V F I C
В выравнивании присутствуют достаточно длинные невыровненные участки (длины 80 на N-конце и длины 50 на C-конце).
Задание 3. Глобальное и локальное парные выравнивания
Были построены четыре парных выравнивания последовательностей WP_063860580.1 и Metallo-beta-lactamase type 2:
Глобальное, полученное из множественного путем удаления лишних последовательностей (см.
fasta-файл )
Launch in Jalview {"seqs":[{"name":"sp|C7C422|BLAN1_KLEPN/1-270","start":1,"end":270,"id":"2023867740","seq":"--------------------------------------------------------------------------MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQRFG-DLVFRQLAPN-VWQ----HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQE-------GMVAAQHSLTF-----AANGWVEPATAPNFGPLKVFYPG--------------PGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGA-----------AFPKASMIVMSHSAP-DSR------------------------------------------------AAITHTARMADKLR----------","order":1},{"name":"gi|1028105449|ref|WP_063860580.1|/1-245","start":1,"end":245,"id":"2079854419","seq":"--------------------------------------------------------------------------------MKKLFVLCIFLFCSIT-----------AAGA-------SLP-DLKIEKLEEG-VYV----HTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWFVER-GYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKD-------GKVQAKNS--F----SGVSYWL----V--KNKIEVFYPG--------------PGHTQDNVVVWLPKNKILFGGCFVKPYG---LGNLDDANVEAWPHSAEKLIS-----------KYGNAKLVVPSHSDI-GDA------------------------------------------------SLLKLTWEQAVKGLNESKKSNTVH","order":2}],"appSettings":{"globalColorScheme":"Blosum62","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.9.0b2"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
sp|C7C422|BLAN1_KLEPN/1-270 gi|1028105449|ref|WP_063860580.1|/1-245 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M E L P N I M H P V A K L S T A L A A A L M L S G C M P G E I R P T I G Q Q M E T G D Q R F G - D L V F R Q L A P N - V W Q - - - - H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E - - - - - - - G M V A A Q H S L T F - - - - - A A N G W V E P A T A P N F G P L K V F Y P G - - - - - - - - - - - - - - P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A - - - - - - - - - - - A F P K A S M I V M S H S A P - D S R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - A A I T H T A R M A D K L R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M K K L F V L C I F L F C S I T - - - - - - - - - - - A A G A - - - - - - - S L P - D L K I E K L E E G - V Y V - - - - H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F V E R - G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D - - - - - - - G K V Q A K N S - - F - - - - S G V S Y W L - - - - V - - K N K I E V F Y P G - - - - - - - - - - - - - - P G H T Q D N V V V W L P K N K I L F G G C F V K P Y G - - - L G N L D D A N V E A W P H S A E K L I S - - - - - - - - - - - K Y G N A K L V V P S H S D I - G D A - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - S L L K L T W E Q A V K G L N E S K K S N T V H - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - * 9 4 9 7 5 * 7 6 7 * 7 9 8 + 7 - - - - - - - - - - - 7 7 * 6 - - - - - - - 6 8 7 - * * 5 8 8 7 * 4 5 7 - * + 6 - - - - * * * 9 5 9 8 8 * 9 * 8 8 8 5 5 * * 9 * 5 5 7 7 7 8 7 9 9 * * 7 9 * 5 6 7 * 4 7 + 9 * * 8 5 8 8 - 7 7 4 9 5 7 8 9 6 9 * 7 * 8 * 5 7 * * 9 9 6 * 5 8 6 8 * 7 * * * 9 8 * 9 * 8 * 7 4 7 9 - - - - - - - * 7 * 6 * 7 5 * - - * - - - - - + 8 9 6 * 9 - - - - 8 - - 6 7 4 + 7 * * * * * - - - - - - - - - - - - - - * * * * 8 * * 9 7 * 6 + 6 5 + 6 * 7 * * * * 8 9 * 6 6 5 - - - * * * * 5 * * 8 7 * 4 + 7 4 * * 8 5 8 7 8 - - - - - - - - - - - 5 9 7 6 * 5 9 9 * 7 * * * 5 6 - 5 7 4 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 8 7 + 6 5 * 6 8 8 * 5 * 7 5 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M E L P N I M + + + + + L + + + L + + + + + L S G C M P G E I R P + + G + Q M E T G D Q + + + - D L + + + + L + + + - V + + - - - - H T S + + + + + G + G + + + + + G L + V + + + + + + + + + D T + + T + + + T + + + + N W + + + + I + + + + + + + + + + H + H + D + + G G + + + L + + + + I + T Y A + + L + N + L + + + + - - - - - - - G + V + A + + S L T F - - - - S + + + + W + E P A T + P N + + + + + V F Y P G - - - - - - - - - - - - - - P G H T + D N + + V + + + + + + I + F G G C + + K + + + A K S L G N L + D A + + E + + + + S A + + + + + - - - - - - - - - - - + + + + A + + + V + S H S + + - + + + - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - + + + + + T + + + A + K + + N E S K K S N T V H
Глобальное, полученное в программе NEEDLE (см. fasta-файл )
Launch in Jalview
{"seqs":[{"name":"sp|C7C422|BLAN1_KLEPN/1-270","start":1,"end":270,"id":"321774541","seq":"MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQRFGDLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHSLTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAFPKASMIVMSHSAPDSRAAITHTARMADKLR----------","order":1},{"name":"gi|1028105449|ref|WP_063860580.1|/1-245","start":1,"end":245,"id":"17586976","seq":"----------MKKLFVLCIFLFCSITAAGASLP--------------DLKIEKLEEGVYVHTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWF-VERGYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKDGKVQAKNSFS-GVSYWLVK------NKIEVFYPGPGHTQDNVVVWLPKNKILFGGCFV---KPYGLGNLDDANVEAWPHSAEKLISKYGNAKLVVPSHSDIGDASLLKLTWEQAVKGLNESKKSNTVH","order":2}],"appSettings":{"globalColorScheme":"Blosum62","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.9.0b2"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
sp|C7C422|BLAN1_KLEPN/1-270 gi|1028105449|ref|WP_063860580.1|/1-245 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 M E L P N I M H P V A K L S T A L A A A L M L S G C M P G E I R P T I G Q Q M E T G D Q R F G D L V F R Q L A P N V W Q H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E G M V A A Q H S L T F A A N G W V E P A T A P N F G P L K V F Y P G P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A A F P K A S M I V M S H S A P D S R A A I T H T A R M A D K L R - - - - - - - - - - - - - - - - - - - - M K K L F V L C I F L F C S I T A A G A S L P - - - - - - - - - - - - - - D L K I E K L E E G V Y V H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F - V E R G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D G K V Q A K N S F S - G V S Y W L V K - - - - - - N K I E V F Y P G P G H T Q D N V V V W L P K N K I L F G G C F V - - - K P Y G L G N L D D A N V E A W P H S A E K L I S K Y G N A K L V V P S H S D I G D A S L L K L T W E Q A V K G L N E S K K S N T V H - - - - - - - - - - 8 * 6 5 6 8 * 9 7 7 * 9 8 * 7 8 8 7 * 4 5 5 * - - - - - - - - - - - - - - * * 5 8 8 7 * 4 5 7 * + 6 * * * 9 5 9 8 8 * 9 * 8 8 8 5 5 * * 9 * 5 5 7 7 7 8 7 9 9 * * 7 9 * 5 6 7 * 4 7 + 9 * * 8 - 6 * 5 7 7 4 9 5 7 8 9 6 9 * 7 * 8 * 5 7 * * 9 9 6 * 5 8 6 8 * 7 * * * 9 8 * 9 * 8 * 7 4 7 9 * 7 * 6 * 7 5 * 8 9 - + 8 9 6 * 9 4 4 - - - - - - 7 4 + 7 * * * * * * * * * 8 * * 9 7 * 6 + 6 5 + 6 * 7 * * * * 8 9 - - - * 7 7 8 * * * * 5 * * 8 7 * 4 + 7 4 * * 8 5 8 7 8 5 9 7 6 * 5 9 9 * 7 * * * 5 6 5 7 4 8 7 + 6 5 * 6 8 8 * 5 * 7 5 - - - - - - - - - - M E L P N I M H P V + K + + + + L + + + L + + S + + + + G + + + P T I G Q Q M E T G D Q R F G D L + + + + L + + + V + + H T S + + + + + G + G + + + + + G L + V + + + + + + + + + D T + + T + + + T + + + + N W + K + E + + + + + + + + + + + H + H + D + + G G + + + L + + + + I + T Y A + + L + N + L + + + + G + V + A + + S + + F + + + + W + + + A T A P N F + + + + V F Y P G P G H T + D N + + V + + + + + + I + F G G C + + K D S K + + + L G N L + D A + + E + + + + S A + + + + + + + + + A + + + V + S H S + + + + + + + + + + T + + + A + K + + N E S K K S N T V H
Локальное, полученное в программе WATER (см. fasta-файл )
Launch in Jalview {"seqs":[{"name":"sp|C7C422|BLAN1_KLEPN/1-204","start":1,"end":204,"id":"1860196218","seq":"DLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHSLTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAFPKASMIVMSHS","order":1},{"name":"gi|1028105449|ref|WP_063860580.1|/1-193","start":1,"end":193,"id":"1925204873","seq":"DLKIEKLEEGVYVHTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWF-VERGYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKDGKVQAKNSFS-GVSYWLVK------NKIEVFYPGPGHTQDNVVVWLPKNKILFGGCFV---KPYGLGNLDDANVEAWPHSAEKLISKYGNAKLVVPSHS","order":2}],"appSettings":{"globalColorScheme":"Blosum62","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.9.0b2"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
sp|C7C422|BLAN1_KLEPN/1-204 gi|1028105449|ref|WP_063860580.1|/1-193 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 D L V F R Q L A P N V W Q H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E G M V A A Q H S L T F A A N G W V E P A T A P N F G P L K V F Y P G P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A A F P K A S M I V M S H S D L K I E K L E E G V Y V H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F - V E R G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D G K V Q A K N S F S - G V S Y W L V K - - - - - - N K I E V F Y P G P G H T Q D N V V V W L P K N K I L F G G C F V - - - K P Y G L G N L D D A N V E A W P H S A E K L I S K Y G N A K L V V P S H S * * 5 8 8 7 * 4 5 7 * + 6 * * * 9 5 9 8 8 * 9 * 8 8 8 5 5 * * 9 * 5 5 7 7 7 8 7 9 9 * * 7 9 * 5 6 7 * 4 7 + 9 * * 8 - 6 * 5 7 7 4 9 5 7 8 9 6 9 * 7 * 8 * 5 7 * * 9 9 6 * 5 8 6 8 * 7 * * * 9 8 * 9 * 8 * 7 4 7 9 * 7 * 6 * 7 5 * 8 9 - + 8 9 6 * 9 4 4 - - - - - - 7 4 + 7 * * * * * * * * * 8 * * 9 7 * 6 + 6 5 + 6 * 7 * * * * 8 9 - - - * 7 7 8 * * * * 5 * * 8 7 * 4 + 7 4 * * 8 5 8 7 8 5 9 7 6 * 5 9 9 * 7 * * * D L + + + + L + + + V + + H T S + + + + + G + G + + + + + G L + V + + + + + + + + + D T + + T + + + T + + + + N W + K + E + + + + + + + + + + + H + H + D + + G G + + + L + + + + I + T Y A + + L + N + L + + + + G + V + A + + S + + F + + + + W + + + A T A P N F + + + + V F Y P G P G H T + D N + + V + + + + + + I + F G G C + + K D S K + + + L G N L + D A + + E + + + + S A + + + + + + + + + A + + + V + S H S
Локальное, полученное в результате работы BLAST (см. mfa-файл )
Launch in Jalview
{"seqs":[{"name":"sp|C7C422|BLAN1_KLEPN/1-204","start":1,"end":204,"id":"1506959950","seq":"DLVFRQLAPNVWQHTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQEGMVAAQHSLTFAANGWVEPATAPNFGPLKVFYPGPGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGAAFPKASMIVMSHS","order":1},{"name":"gi|1028105449|ref|WP_063860580.1|/1-193","start":1,"end":193,"id":"1801704649","seq":"DLKIEKLEEGVYVHTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWF-VERGYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKDGKVQAKNSFSGVSYWLVK-------NKIEVFYPGPGHTQDNVVVWLPKNKILFGGCFV---KPYGLGNLDDANVEAWPHSAEKLISKYGNAKLVVPSHS","order":2}],"appSettings":{"globalColorScheme":"Blosum62","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.9.0b2"},"seqGroups":[],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
sp|C7C422|BLAN1_KLEPN/1-204 gi|1028105449|ref|WP_063860580.1|/1-193 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 D L V F R Q L A P N V W Q H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E G M V A A Q H S L T F A A N G W V E P A T A P N F G P L K V F Y P G P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A A F P K A S M I V M S H S D L K I E K L E E G V Y V H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F - V E R G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D G K V Q A K N S F S G V S Y W L V K - - - - - - - N K I E V F Y P G P G H T Q D N V V V W L P K N K I L F G G C F V - - - K P Y G L G N L D D A N V E A W P H S A E K L I S K Y G N A K L V V P S H S * * 5 8 8 7 * 4 5 7 * + 6 * * * 9 5 9 8 8 * 9 * 8 8 8 5 5 * * 9 * 5 5 7 7 7 8 7 9 9 * * 7 9 * 5 6 7 * 4 7 + 9 * * 8 - 6 * 5 7 7 4 9 5 7 8 9 6 9 * 7 * 8 * 5 7 * * 9 9 6 * 5 8 6 8 * 7 * * * 9 8 * 9 * 8 * 7 4 7 9 * 7 * 6 * 7 5 * 8 9 7 8 8 7 6 7 * 7 - - - - - - - 7 4 + 7 * * * * * * * * * 8 * * 9 7 * 6 + 6 5 + 6 * 7 * * * * 8 9 - - - * 7 7 8 * * * * 5 * * 8 7 * 4 + 7 4 * * 8 5 8 7 8 5 9 7 6 * 5 9 9 * 7 * * * D L + + + + L + + + V + + H T S + + + + + G + G + + + + + G L + V + + + + + + + + + D T + + T + + + T + + + + N W + K + E + + + + + + + + + + + H + H + D + + G G + + + L + + + + I + T Y A + + L + N + L + + + + G + V + A + + S + + + + + + + + V + P A T A P N F + + + + V F Y P G P G H T + D N + + V + + + + + + I + F G G C + + K D S K + + + L G N L + D A + + E + + + + S A + + + + + + + + + A + + + V + S H S
Проект JalView
Задание 4. Выравнивание различных выравниваний друг относительно друга
На картинке несовпадающие участки выравнивания.
Участки, найденные программами BLAST и WATER, совпадают.
Launch in Jalview
{"seqs":[{"name":"sp|C7C422|BLAN1_KLEPN/1-204","start":1,"end":204,"id":"718302750","seq":"--------------------------------------------------------------------------------------------------------------------------DLVFRQLAPN-VWQ----HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQE-------GMVAAQHSLTF-----AANGWVEPATAPNFGPLKVFYPG--------------PGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGA-----------AFPKASMIVMSHS------------------------------------------------------------------------------","order":1},{"name":"gi|1028105449|ref|WP_063860580.1|/1-193","start":1,"end":193,"id":"1054271325","seq":"--------------------------------------------------------------------------------------------------------------------------DLKIEKLEEG-VYV----HTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWF-VERGYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKD-------GKVQAKNSFSG-----VSYWLVK-------NKIEVFYPG--------------PGHTQDNVVVWLPKNKILFGGCFV---KPYGLGNLDDANVEAWPHSAEKLIS-----------KYGNAKLVVPSHS------------------------------------------------------------------------------","order":2},{"name":"sp|C7C422|BLAN1_KLEPN/1-204","start":1,"end":204,"id":"908595978","seq":"--------------------------------------------------------------------------------------------------------------------------DLVFRQLAPN-VWQ----HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQE-------GMVAAQHSLTF-----AANGWVEPATAPNFGPLKVFYPG--------------PGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGA-----------AFPKASMIVMSHS------------------------------------------------------------------------------","order":3},{"name":"gi|1028105449|ref|WP_063860580.1|/1-193","start":1,"end":193,"id":"1572524246","seq":"--------------------------------------------------------------------------------------------------------------------------DLKIEKLEEG-VYV----HTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWF-VERGYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKD-------GKVQAKNSFS------GVSYWLVK------NKIEVFYPG--------------PGHTQDNVVVWLPKNKILFGGCFV---KPYGLGNLDDANVEAWPHSAEKLIS-----------KYGNAKLVVPSHS------------------------------------------------------------------------------","order":4},{"name":"sp|C7C422|BLAN1_KLEPN/1-270","start":1,"end":270,"id":"1949562337","seq":"--------------------------------------------------------------------------MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQRFG-DLVFRQLAPN-VWQ----HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQE-------GMVAAQHSLTF-----AANGWVEPATAPNFGPLKVFYPG--------------PGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGA-----------AFPKASMIVMSHSAP-DSR------------------------------------------------AAITHTARMADKLR----------","order":5},{"name":"gi|1028105449|ref|WP_063860580.1|/1-245","start":1,"end":245,"id":"2111178805","seq":"--------------------------------------------------------------------------------MKKLFVLCIFLFCSIT-----------AAGA-------SLP-DLKIEKLEEG-VYV----HTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWFVER-GYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKD-------GKVQAKNS--F----SGVSYWL----V--KNKIEVFYPG--------------PGHTQDNVVVWLPKNKILFGGCFVKPYG---LGNLDDANVEAWPHSAEKLIS-----------KYGNAKLVVPSHSDI-GDA------------------------------------------------SLLKLTWEQAVKGLNESKKSNTVH","order":6},{"name":"sp|C7C422|BLAN1_KLEPN/1-270","start":1,"end":270,"id":"194045126","seq":"--------------------------------------------------------------------------MELPNIMHPVAKLSTALAAALMLSGCMPGEIRPTIGQQMETGDQRFG-DLVFRQLAPN-VWQ----HTSYLDMPGFGAVASNGLIVRDGGRVLVVDTAWTDDQTAQILNWIKQEINLPVALAVVTHAHQDKMGGMDALHAAGIATYANALSNQLAPQE-------GMVAAQHSLTF-----AANGWVEPATAPNFGPLKVFYPG--------------PGHTSDNITVGIDGTDIAFGGCLIKDSKAKSLGNLGDADTEHYAASARAFGA-----------AFPKASMIVMSHSAP-DSR------------------------------------------------AAITHTARMADKLR----------","order":7},{"name":"gi|1028105449|ref|WP_063860580.1|/1-245","start":1,"end":245,"id":"10991874","seq":"------------------------------------------------------------------------------------MKKLFVLCIFLFCSITAAGASLP---------------DLKIEKLEEG-VYV----HTSFEEVNGWGVASKHGLVVLVNTDAYLIDTPFTAKDTEKLVNWF-VERGYKIKGSISSHFHSDSTGGIEWLNSQSIPTYASVLTNELLKKD-------GKVQAKNSFS------GVSYWLVK------NKIEVFYPG--------------PGHTQDNVVVWLPKNKILFGGCFV---KPYGLGNLDDANVEAWPHSAEKLIS-----------KYGNAKLVVPSHSDI-GDA------------------------------------------------SLLKLTWEQAVKGLNESKKSNTVH","order":8}],"appSettings":{"globalColorScheme":"Blosum62","webStartUrl":"http://www.jalview.org/services/launchApp","application":"Jalview","showSeqFeatures":"false","version":"2.9.0b2"},"seqGroups":[{"displayText":true,"groupName":"JGroup:526728933","sequenceRefs":["1949562337","2111178805"],"startRes":422,"endRes":445,"colourText":false,"showNonconserved":false,"colourScheme":"Blosum62","displayBoxes":true},{"displayText":true,"groupName":"JGroup:1029751795","sequenceRefs":["194045126","10991874"],"startRes":0,"endRes":445,"colourText":false,"showNonconserved":false,"colourScheme":"Blosum62","displayBoxes":true},{"displayText":true,"groupName":"JGroup:1094620197","sequenceRefs":["1949562337","2111178805"],"startRes":0,"endRes":445,"colourText":false,"showNonconserved":false,"colourScheme":"Blosum62","displayBoxes":true},{"displayText":true,"groupName":"JGroup:820914219","sequenceRefs":["908595978","1572524246"],"startRes":0,"endRes":445,"colourText":false,"showNonconserved":false,"colourScheme":"Blosum62","displayBoxes":true},{"displayText":true,"groupName":"JGroup:1616479117","sequenceRefs":["718302750","1054271325"],"startRes":0,"endRes":445,"colourText":false,"showNonconserved":false,"colourScheme":"Blosum62","displayBoxes":true}],"alignAnnotation":[],"svid":"1.0","seqFeatures":[]}
sp|C7C422|BLAN1_KLEPN/1-204 gi|1028105449|ref|WP_063860580.1|/1-193 sp|C7C422|BLAN1_KLEPN/1-204 gi|1028105449|ref|WP_063860580.1|/1-193 sp|C7C422|BLAN1_KLEPN/1-270 gi|1028105449|ref|WP_063860580.1|/1-245 sp|C7C422|BLAN1_KLEPN/1-270 gi|1028105449|ref|WP_063860580.1|/1-245 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L V F R Q L A P N - V W Q - - - - H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E - - - - - - - G M V A A Q H S L T F - - - - - A A N G W V E P A T A P N F G P L K V F Y P G - - - - - - - - - - - - - - P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A - - - - - - - - - - - A F P K A S M I V M S H S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L K I E K L E E G - V Y V - - - - H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F - V E R G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D - - - - - - - G K V Q A K N S F S G - - - - - V S Y W L V K - - - - - - - N K I E V F Y P G - - - - - - - - - - - - - - P G H T Q D N V V V W L P K N K I L F G G C F V - - - K P Y G L G N L D D A N V E A W P H S A E K L I S - - - - - - - - - - - K Y G N A K L V V P S H S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L V F R Q L A P N - V W Q - - - - H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E - - - - - - - G M V A A Q H S L T F - - - - - A A N G W V E P A T A P N F G P L K V F Y P G - - - - - - - - - - - - - - P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A - - - - - - - - - - - A F P K A S M I V M S H S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - D L K I E K L E E G - V Y V - - - - H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F - V E R G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D - - - - - - - G K V Q A K N S F S - - - - - - G V S Y W L V K - - - - - - N K I E V F Y P G - - - - - - - - - - - - - - P G H T Q D N V V V W L P K N K I L F G G C F V - - - K P Y G L G N L D D A N V E A W P H S A E K L I S - - - - - - - - - - - K Y G N A K L V V P S H S - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M E L P N I M H P V A K L S T A L A A A L M L S G C M P G E I R P T I G Q Q M E T G D Q R F G - D L V F R Q L A P N - V W Q - - - - H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E - - - - - - - G M V A A Q H S L T F - - - - - A A N G W V E P A T A P N F G P L K V F Y P G - - - - - - - - - - - - - - P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A - - - - - - - - - - - A F P K A S M I V M S H S A P - D S R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - A A I T H T A R M A D K L R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M K K L F V L C I F L F C S I T - - - - - - - - - - - A A G A - - - - - - - S L P - D L K I E K L E E G - V Y V - - - - H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F V E R - G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D - - - - - - - G K V Q A K N S - - F - - - - S G V S Y W L - - - - V - - K N K I E V F Y P G - - - - - - - - - - - - - - P G H T Q D N V V V W L P K N K I L F G G C F V K P Y G - - - L G N L D D A N V E A W P H S A E K L I S - - - - - - - - - - - K Y G N A K L V V P S H S D I - G D A - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - S L L K L T W E Q A V K G L N E S K K S N T V H - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M E L P N I M H P V A K L S T A L A A A L M L S G C M P G E I R P T I G Q Q M E T G D Q R F G - D L V F R Q L A P N - V W Q - - - - H T S Y L D M P G F G A V A S N G L I V R D G G R V L V V D T A W T D D Q T A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E - - - - - - - G M V A A Q H S L T F - - - - - A A N G W V E P A T A P N F G P L K V F Y P G - - - - - - - - - - - - - - P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A - - - - - - - - - - - A F P K A S M I V M S H S A P - D S R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - A A I T H T A R M A D K L R - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M K K L F V L C I F L F C S I T A A G A S L P - - - - - - - - - - - - - - - D L K I E K L E E G - V Y V - - - - H T S F E E V N G W G V A S K H G L V V L V N T D A Y L I D T P F T A K D T E K L V N W F - V E R G Y K I K G S I S S H F H S D S T G G I E W L N S Q S I P T Y A S V L T N E L L K K D - - - - - - - G K V Q A K N S F S - - - - - - G V S Y W L V K - - - - - - N K I E V F Y P G - - - - - - - - - - - - - - P G H T Q D N V V V W L P K N K I L F G G C F V - - - K P Y G L G N L D D A N V E A W P H S A E K L I S - - - - - - - - - - - K Y G N A K L V V P S H S D I - G D A - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - S L L K L T W E Q A V K G L N E S K K S N T V H - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - * * 5 8 8 7 * 4 5 7 - * + 6 - - - - * * * 9 5 9 8 8 * 9 * 8 8 8 5 5 * * 9 * 5 5 7 7 7 8 7 9 9 * * 7 9 * 5 6 7 * 4 7 + 9 * * 8 - 4 8 0 7 7 4 9 5 7 8 9 6 9 * 7 * 8 * 5 7 * * 9 9 6 * 5 8 6 8 * 7 * * * 9 8 * 9 * 8 * 7 4 7 9 - - - - - - - * 7 * 6 * 7 5 * 1 2 - - - - - - 8 6 6 6 7 9 0 - - - - - - - 7 4 + 7 * * * * * - - - - - - - - - - - - - - * * * * 8 * * 9 7 * 6 + 6 5 + 6 * 7 * * * * 8 9 - - - 5 1 2 2 * * * * 5 * * 8 7 * 4 + 7 4 * * 8 5 8 7 8 - - - - - - - - - - - 5 9 7 6 * 5 9 9 * 7 * * * - - - 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Проект JalView
Задание 5. Парные выравнивания поледовательностей негомологичных белков
Глобальное, полученное в программе NEEDLE (см. fasta-файл )
Локальное, полученное в программе WATER (см. fasta-файл )
Выравнивание друг отностительно друга
Launch in Jalview
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sp|C7C422|BLAN1_KLEPN/1-270 4R1L:A|PDBID|CHAIN|SEQUENCE/1-436 sp|C7C422|BLAN1_KLEPN/1-97 4R1L:A|PDBID|CHAIN|SEQUENCE/1-90 Conservation Quality Consensus
10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250 260 270 280 290 300 310 320 330 340 350 360 370 380 390 400 410 420 430 440 450 460 470 480 490 500 510 520 530 540 550 560 570 580 - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M E L P N I M H P V A K L S T A L A A A L M L S G C M P G E I - - - R P T I G Q Q M E T G D Q R F - - - G D L V F R Q L A P N V W Q H T S Y - - - - L D M P G - - F G A V A S N G L I V R D - - - G G R V L V V D T A W T D D Q T - - - - - - - - - - A Q I L N W I K Q E I N L P V A L A V V T H A H Q D K M G G M D A L H A A G I A T Y A N A L S N Q L A P Q E G M V A A Q H S L T F A A N G W V E P A T A P N F G P L K V F Y P G P G H T S D N I T V G I D G T D I A F G G C L I K D S K A K S L G N L G D A D T E H Y A A S A R A F G A A F P K A S M I V M S H S A P D S R A A I T H T A R M A D K L R G M S T Q Y W E E E I E I M S R E K L Q E L Q L Q R L K K T I N I A A N S P Y Y K E V F S K N G I T G D S I Q S L D D I R K I P F T T K S D M R A N Y P F G L V A G D M K R D G V R I H S S S G T T G N P T V I V H S Q H D L D S W A N L V A R C L Y M V G I R K T D V F Q N S S G Y G M F T G G L G F Q Y G A E R L G C L T V P A A A G N S K R Q I K F I S D F K T T A L H A I P S Y A I R L A E V F Q E E G I D P R E T T L K T L V I G A E P H T D E Q R R K I E R M L N V K A Y N S F G M T E M N G P G V A F E C Q E Q N G M H F W E D C Y L V E I I D P E T G E P V P E G E I G E L V L T T L D R E M M P L I R Y R T R D L T R I L P G K C P C G R T - - - H L R I D R I K G R S D D M F I I K G V N I F P M Q V E K I - - - - - - - - - - - - L V Q - - F P E L G S N Y L I T L E T V N N Q D E M I V E V E L S D L S T D N Y I E L E K I R R D I I R Q L K D E I L V T P K V K L V - - - - - - K K G S L P - - Q S E G K A V R V K D L R D N K - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - M H P V A K L S T A L A A A L M L S G C M P G E I - - - R P T I G Q Q M E T G D Q R F - - - G D L V F R Q L A P N V W Q H T S Y - - - - L D M P G - - F G A V A S N G L I V R D - - - G G R V L V V D T A W T D D Q T - - - - - - - - - - A Q I L N - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - 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