[M::bwa_idx_load_from_disk] read 0 ALT contigs [M::process] read 1620666 sequences (120000036 bp)... [M::process] read 1622138 sequences (120000032 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 32224, 12, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 512, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3341) [M::mem_pestat] mean and std.dev: (651.07, 543.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367) [M::mem_pestat] mean and std.dev: (186.41, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 297, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4706) [M::mem_pestat] mean and std.dev: (565.00, 625.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1620666 reads in 241.344 CPU sec, 19.930 real sec [M::process] read 1621560 sequences (120000136 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 31756, 14, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367) [M::mem_pestat] mean and std.dev: (186.23, 52.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (124, 585, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5011) [M::mem_pestat] mean and std.dev: (451.64, 492.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6640) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1622138 reads in 232.055 CPU sec, 19.021 real sec [M::process] read 1622932 sequences (120000109 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 31955, 18, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (186.61, 53.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 268, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256) [M::mem_pestat] mean and std.dev: (378.35, 335.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1621560 reads in 232.230 CPU sec, 19.104 real sec [M::process] read 1623234 sequences (120000053 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 32278, 13, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (476, 1207, 2255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5813) [M::mem_pestat] mean and std.dev: (1401.90, 1318.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.54, 52.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 1634, 7457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21895) [M::mem_pestat] mean and std.dev: (3558.23, 3451.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29114) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1622932 reads in 232.289 CPU sec, 19.076 real sec [M::process] read 1623366 sequences (120000033 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 31929, 17, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1096, 2784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7636) [M::mem_pestat] mean and std.dev: (1500.09, 1258.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.92, 53.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 562, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3863) [M::mem_pestat] mean and std.dev: (690.69, 602.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5084) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1623234 reads in 231.164 CPU sec, 18.975 real sec [M::process] read 1622994 sequences (120000039 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 32100, 18, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (515, 2905, 3976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10898) [M::mem_pestat] mean and std.dev: (2315.53, 1970.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (186.64, 53.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 886, 1636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4362) [M::mem_pestat] mean and std.dev: (653.79, 569.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5725) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1623366 reads in 231.019 CPU sec, 19.035 real sec [M::process] read 1624146 sequences (120000068 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 32202, 23, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.11, 53.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 193, 5993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17827) [M::mem_pestat] mean and std.dev: (2339.26, 3560.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23744) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1622994 reads in 230.112 CPU sec, 18.811 real sec [M::process] read 1623154 sequences (120000042 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 32180, 19, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 528, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5229) [M::mem_pestat] mean and std.dev: (1131.46, 1533.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.68, 53.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 260, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7690) [M::mem_pestat] mean and std.dev: (1679.05, 2492.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 2070, 2929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8413) [M::mem_pestat] mean and std.dev: (1575.20, 1391.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1624146 reads in 229.604 CPU sec, 19.042 real sec [M::process] read 1623222 sequences (120000026 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 32040, 9, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.24, 53.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 1623154 reads in 229.558 CPU sec, 18.811 real sec [M::process] read 1622724 sequences (120000078 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 31600, 12, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.72, 53.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 421, 1644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4742) [M::mem_pestat] mean and std.dev: (635.82, 808.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6291) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1623222 reads in 229.429 CPU sec, 18.905 real sec [M::process] read 1624184 sequences (120000036 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 32123, 17, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.78, 53.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 909, 6192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18448) [M::mem_pestat] mean and std.dev: (2599.88, 2898.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24576) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1622724 reads in 232.027 CPU sec, 18.921 real sec [M::process] read 1623932 sequences (120000024 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 32279, 15, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (187.13, 53.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 307, 6000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17730) [M::mem_pestat] mean and std.dev: (2260.07, 2925.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1177, 1415, 2794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6028) [M::mem_pestat] mean and std.dev: (1786.91, 894.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7645) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1624184 reads in 229.807 CPU sec, 18.896 real sec [M::process] read 1623636 sequences (120000025 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 31948, 16, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367) [M::mem_pestat] mean and std.dev: (186.31, 53.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 758, 6720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19638) [M::mem_pestat] mean and std.dev: (2279.44, 2936.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26097) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1623932 reads in 229.126 CPU sec, 18.768 real sec [M::process] read 1624710 sequences (120000088 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 31736, 16, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 365) [M::mem_pestat] mean and std.dev: (186.33, 52.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1421, 7457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21821) [M::mem_pestat] mean and std.dev: (2808.19, 3124.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29003) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1623636 reads in 229.194 CPU sec, 18.712 real sec [M::process] read 1624504 sequences (120000039 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 32108, 13, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (2, 367) [M::mem_pestat] mean and std.dev: (186.54, 53.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 440) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (165, 529, 6756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19938) [M::mem_pestat] mean and std.dev: (2336.31, 2924.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26529) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1624710 reads in 229.152 CPU sec, 18.763 real sec [M::process] read 1624254 sequences (120000044 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 31868, 18, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (494, 743, 3019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8069) [M::mem_pestat] mean and std.dev: (1575.00, 1430.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (187.26, 54.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 800, 5591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16537) [M::mem_pestat] mean and std.dev: (2323.83, 2910.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (595, 1087, 2393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5989) [M::mem_pestat] mean and std.dev: (1756.07, 1704.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1624504 reads in 229.529 CPU sec, 18.817 real sec [M::process] read 1625416 sequences (120000037 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 31882, 18, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1102, 1274, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5677) [M::mem_pestat] mean and std.dev: (1617.73, 1183.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 365) [M::mem_pestat] mean and std.dev: (186.45, 52.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (116, 558, 2939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8585) [M::mem_pestat] mean and std.dev: (1826.06, 2420.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1045, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (838.60, 358.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1624254 reads in 231.171 CPU sec, 18.973 real sec [M::process] read 1625072 sequences (120000020 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 32060, 15, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.76, 53.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 132, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514) [M::mem_pestat] mean and std.dev: (458.73, 486.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1625416 reads in 229.041 CPU sec, 18.840 real sec [M::process] read 1625476 sequences (120000133 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 31958, 21, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 1592, 3652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10432) [M::mem_pestat] mean and std.dev: (2229.90, 2094.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.32, 53.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 257, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4127) [M::mem_pestat] mean and std.dev: (513.63, 735.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1625072 reads in 228.815 CPU sec, 18.782 real sec [M::process] read 1627150 sequences (120000094 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 31930, 14, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.89, 52.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 1441, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4515) [M::mem_pestat] mean and std.dev: (841.91, 674.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5942) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1625476 reads in 232.873 CPU sec, 19.174 real sec [M::process] read 1625950 sequences (120000064 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 31926, 10, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1148, 1252, 3400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7904) [M::mem_pestat] mean and std.dev: (1803.07, 1609.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.72, 53.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 158, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (384.89, 492.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (609, 881, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2673) [M::mem_pestat] mean and std.dev: (922.62, 440.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1627150 reads in 238.604 CPU sec, 19.674 real sec [M::process] read 1628062 sequences (120000030 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 31993, 5, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.49, 53.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 1625950 reads in 237.107 CPU sec, 19.440 real sec [M::process] read 1626910 sequences (120000020 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 31984, 16, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 606, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3020) [M::mem_pestat] mean and std.dev: (648.38, 490.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (187.15, 53.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 1419, 2843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8101) [M::mem_pestat] mean and std.dev: (1857.25, 2213.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (671, 1581, 2333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5657) [M::mem_pestat] mean and std.dev: (1855.90, 1569.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1628062 reads in 238.282 CPU sec, 19.515 real sec [M::process] read 1625904 sequences (120000114 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 31652, 16, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 181, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (188.40, 54.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 553, 5988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17846) [M::mem_pestat] mean and std.dev: (1937.50, 2577.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1626910 reads in 236.321 CPU sec, 19.373 real sec [M::process] read 1630146 sequences (120000050 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 31715, 17, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (718, 1255, 2283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5413) [M::mem_pestat] mean and std.dev: (1373.70, 1019.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (188.02, 54.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 208, 1663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4845) [M::mem_pestat] mean and std.dev: (448.13, 678.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6436) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1625904 reads in 236.221 CPU sec, 19.347 real sec [M::process] read 1637394 sequences (120000090 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 32156, 20, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (114, 663, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3408) [M::mem_pestat] mean and std.dev: (457.38, 464.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (150, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 369) [M::mem_pestat] mean and std.dev: (187.54, 53.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 588, 6051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17767) [M::mem_pestat] mean and std.dev: (2214.70, 2808.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (501, 2124, 5677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16029) [M::mem_pestat] mean and std.dev: (2685.08, 2309.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1630146 reads in 230.168 CPU sec, 19.022 real sec [M::process] read 1638698 sequences (120000125 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 31983, 21, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1064, 2280, 3390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8042) [M::mem_pestat] mean and std.dev: (2194.33, 1476.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (188.03, 53.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 886, 6003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17707) [M::mem_pestat] mean and std.dev: (2243.29, 2677.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23559) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1637394 reads in 230.491 CPU sec, 19.118 real sec [M::process] read 1634848 sequences (120000099 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 32009, 14, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 532, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3149) [M::mem_pestat] mean and std.dev: (696.33, 792.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.44, 53.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 106, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (183.08, 240.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1092, 2966, 3396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8004) [M::mem_pestat] mean and std.dev: (2606.60, 1847.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1638698 reads in 230.875 CPU sec, 18.806 real sec [M::process] read 1637040 sequences (120000142 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 32062, 17, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.80, 53.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 808, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4137) [M::mem_pestat] mean and std.dev: (616.93, 566.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (568, 1152, 2304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5776) [M::mem_pestat] mean and std.dev: (1457.15, 1034.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7512) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1634848 reads in 229.760 CPU sec, 18.939 real sec [M::process] read 1634640 sequences (120000127 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 31934, 17, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.48, 53.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 800, 7457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21985) [M::mem_pestat] mean and std.dev: (2800.53, 3344.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1637040 reads in 229.921 CPU sec, 18.875 real sec [M::process] read 1636374 sequences (120000108 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 32023, 20, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (804, 1171, 2412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5628) [M::mem_pestat] mean and std.dev: (1619.24, 1210.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (186.96, 53.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (523, 886, 2675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6979) [M::mem_pestat] mean and std.dev: (1871.25, 2191.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10637) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1634640 reads in 242.531 CPU sec, 19.973 real sec [M::process] read 1633804 sequences (120000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 31983, 12, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (187.09, 53.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (90, 873, 2055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5985) [M::mem_pestat] mean and std.dev: (652.30, 703.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (836, 1294, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4208) [M::mem_pestat] mean and std.dev: (1283.20, 737.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5332) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1636374 reads in 244.086 CPU sec, 20.026 real sec [M::process] read 1635154 sequences (120000110 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 31750, 13, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.46, 53.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 396, 3873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11579) [M::mem_pestat] mean and std.dev: (1983.00, 2751.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1633804 reads in 244.489 CPU sec, 20.059 real sec [M::process] read 1632612 sequences (120000092 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 31942, 18, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (476, 1354, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7730) [M::mem_pestat] mean and std.dev: (1623.17, 1652.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.26, 53.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (124, 542, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4000) [M::mem_pestat] mean and std.dev: (611.25, 642.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1635154 reads in 246.851 CPU sec, 20.157 real sec [M::process] read 1632114 sequences (120000061 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 32121, 23, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (187.27, 53.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 644, 1577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4157) [M::mem_pestat] mean and std.dev: (802.85, 801.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (525, 1192, 3022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8016) [M::mem_pestat] mean and std.dev: (2063.00, 1944.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10513) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1632612 reads in 242.905 CPU sec, 20.118 real sec [M::process] read 1634138 sequences (120000130 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 32163, 13, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1194, 1714, 5643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14541) [M::mem_pestat] mean and std.dev: (2676.00, 2239.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.95, 53.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 314, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (339.73, 332.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1632114 reads in 258.471 CPU sec, 21.229 real sec [M::process] read 1632676 sequences (120000061 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 32395, 16, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (577, 1306, 3603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9655) [M::mem_pestat] mean and std.dev: (1700.33, 1387.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (187.05, 52.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 323, 4354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12956) [M::mem_pestat] mean and std.dev: (1970.12, 3167.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1099, 1289, 2666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5800) [M::mem_pestat] mean and std.dev: (1746.06, 1301.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1634138 reads in 255.737 CPU sec, 21.062 real sec [M::process] read 1635438 sequences (120000087 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 32121, 12, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.99, 53.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 289, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2258) [M::mem_pestat] mean and std.dev: (457.42, 489.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 1191, 2287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6329) [M::mem_pestat] mean and std.dev: (1281.64, 1201.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8350) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1632676 reads in 250.274 CPU sec, 20.566 real sec [M::process] read 1631314 sequences (120000032 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 32044, 18, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (526, 1110, 3026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8026) [M::mem_pestat] mean and std.dev: (1538.60, 1410.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.59, 52.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 153, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569) [M::mem_pestat] mean and std.dev: (262.80, 364.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3406) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1635438 reads in 243.394 CPU sec, 19.978 real sec [M::process] read 1633512 sequences (120000062 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 32206, 12, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.84, 52.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 264, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (222.00, 209.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2067) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1631314 reads in 239.545 CPU sec, 19.631 real sec [M::process] read 1632380 sequences (120000005 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 32221, 15, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (562, 840, 2284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (1283.06, 1070.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.86, 53.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 306, 1659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4567) [M::mem_pestat] mean and std.dev: (849.23, 1122.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6021) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1633512 reads in 244.213 CPU sec, 20.067 real sec [M::process] read 1632542 sequences (120000095 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 32409, 20, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 508, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1261) [M::mem_pestat] mean and std.dev: (564.00, 354.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.83, 53.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 574, 3866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11212) [M::mem_pestat] mean and std.dev: (2078.45, 2916.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (442, 1985, 3041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8239) [M::mem_pestat] mean and std.dev: (1828.27, 1455.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1632380 reads in 241.355 CPU sec, 19.859 real sec [M::process] read 1635014 sequences (120000088 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 31991, 12, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (186.54, 53.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (508, 802, 7457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21355) [M::mem_pestat] mean and std.dev: (3113.33, 3283.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 1242, 3216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8254) [M::mem_pestat] mean and std.dev: (1913.54, 1507.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10773) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1632542 reads in 258.597 CPU sec, 21.151 real sec [M::process] read 1632390 sequences (120000098 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 32070, 18, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 570, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348) [M::mem_pestat] mean and std.dev: (779.71, 573.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.91, 52.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 115, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (182.31, 234.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1635014 reads in 270.201 CPU sec, 22.235 real sec [M::process] read 1634158 sequences (120000057 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 32340, 13, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (548, 1144, 2737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7115) [M::mem_pestat] mean and std.dev: (1502.09, 1310.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.82, 52.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 395, 6479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19051) [M::mem_pestat] mean and std.dev: (2221.46, 3257.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1632390 reads in 269.536 CPU sec, 22.135 real sec [M::process] read 1630588 sequences (120000030 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 32127, 18, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1229, 3614, 5649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14489) [M::mem_pestat] mean and std.dev: (3128.00, 2061.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (186.79, 53.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 1425, 5989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17747) [M::mem_pestat] mean and std.dev: (2528.11, 3175.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1634158 reads in 264.570 CPU sec, 21.573 real sec [M::process] read 1630988 sequences (120000055 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 32076, 22, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 628, 3026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8830) [M::mem_pestat] mean and std.dev: (1514.69, 1819.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11732) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 365) [M::mem_pestat] mean and std.dev: (186.71, 52.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 886, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3901) [M::mem_pestat] mean and std.dev: (712.47, 594.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (482, 1023, 3016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8084) [M::mem_pestat] mean and std.dev: (1507.65, 1303.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1630588 reads in 264.843 CPU sec, 21.892 real sec [M::process] read 1632046 sequences (120000073 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 32033, 18, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 365) [M::mem_pestat] mean and std.dev: (186.19, 52.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 249, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748) [M::mem_pestat] mean and std.dev: (300.25, 276.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2302) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1630988 reads in 254.494 CPU sec, 20.917 real sec [M::process] read 1630322 sequences (120000041 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 32111, 17, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (561, 1195, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4080) [M::mem_pestat] mean and std.dev: (1187.83, 911.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5253) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 180, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 368) [M::mem_pestat] mean and std.dev: (187.18, 53.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 7457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22247) [M::mem_pestat] mean and std.dev: (2472.00, 3533.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29642) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1217, 1790, 3625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8441) [M::mem_pestat] mean and std.dev: (2329.64, 1501.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1632046 reads in 253.343 CPU sec, 20.844 real sec [M::process] read 895714 sequences (65924653 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 32362, 15, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (149, 179, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 365) [M::mem_pestat] mean and std.dev: (186.43, 52.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 571, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490) [M::mem_pestat] mean and std.dev: (449.93, 373.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3292) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1079, 1398, 2940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6662) [M::mem_pestat] mean and std.dev: (1813.25, 1334.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8523) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1630322 reads in 255.713 CPU sec, 21.096 real sec [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 17736, 11, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (148, 178, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 370) [M::mem_pestat] mean and std.dev: (186.46, 53.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (523, 608, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205) [M::mem_pestat] mean and std.dev: (702.90, 539.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4099) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 895714 reads in 140.987 CPU sec, 11.859 real sec [main] Version: 0.7.17-r1188 [main] CMD: bwa mem -t 12 ../bwa/chr8.fna ../dna_seq/out_paired_SRR10720419_1.fastq.gz ../dna_seq/out_paired_SRR10720419_2.fastq.gz [main] Real time: 1014.771 sec; CPU: 12148.002 sec