1: Plant Mol Biol. 2001 Dec;47(6):739-48.

Differential expression of putative cell death regulator genes in near-isogenic, 
resistant and susceptible barley lines during interaction with the powdery mildew
fungus.

Hückelhoven R, Dechert C, Trujillo M, Kogel KH.

Institute of Phytopathology and Applied Zoology, Justus-Liebig-Universität
Giessen, Germany. Ralph.Hueckelhoven@agrar.uni-giessen.de

We analysed pathogenesis-related expression of genes, that are assumed to be
involved in ubiquitous plant defence mechanisms like the oxidative burst, the
hypersensitive cell death reaction (HR) and formation of localized cell wall
appositions (papillae). We carried out comparative northern blot and RT-PCR
studies with near-isogenic barley (Hordeum vulgareL. cv. Pallas) lines (NILs)
resistant or susceptible to the powdery mildew fungus race A6 (Blumeria graminis 
f.sp. hordei, BghA6). The NILs carrying one of the R-genes Mla12, Mlg or the mlo 
mutant allele mlo5 arrest fungal development by cell wall appositions (mlo5) or a
HR (Mla12) or both (Mlg). Expression of an aspartate protease gene, an ascorbate 
peroxidase gene and a newly identified cysteine protease gene was up-regulated
after inoculation with BghA6, whereas the constitutive expression-level of a BAS 
gene, that encodes an alkyl hydroperoxide reductase, was reduced. Expression of a
newly identified barley homologue of a mammalian cell death regulator, Bax
inhibitor 1, was enhanced after powdery mildew inoculation. An oxalate
oxidase-like protein was stronger expressed in NILS expressing penetration
resistance. A so far unknown gene that putatively encodes the large subunit of a 
superoxide generating NADPH oxidases was constitutively expressed in barley
leaves and its expression pattern did not change after inoculation. A newly
identified barley Rac1 homologue was expressed constitutively, such as the
functionally linked NADPH oxidase gene. Gene expression patterns are discussed
with regard to defence mechanisms and signal transduction.


PMID: 11785935 [PubMed - indexed for MEDLINE]

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