ID   ENGB_BACSU              Reviewed;         195 AA.
AC   P38424;
DT   01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1994, sequence version 1.
DT   25-JAN-2012, entry version 91.
DE   RecName: Full=Probable GTP-binding protein EngB;
GN   Name=engB; Synonyms=ysxC; OrderedLocusNames=BSU28190;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168 / IS58;
RX   MEDLINE=95050209; PubMed=7961402;
RA   Riethdorf S., Voelker U., Gerth U., Winkler A., Engelmann S.,
RA   Hecker M.;
RT   "Cloning, nucleotide sequence, and expression of the Bacillus subtilis
RT   lon gene.";
RL   J. Bacteriol. 176:6518-6527(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=97124191; PubMed=8969504;
RA   Wipat A., Carter N., Brignell C.S., Guy J.B., Piper K., Sanders J.,
RA   Emmerson P.T., Harwood C.R.;
RT   "The dnaB-pheA (256 degrees-240 degrees) region of the Bacillus
RT   subtilis chromosome containing genes responsible for stress responses,
RT   the utilization of plant cell walls and primary metabolism.";
RL   Microbiology 142:3067-3078(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   MEDLINE=98044033; PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G.,
RA   Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S.,
RA   Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S.,
RA   Brouillet S., Bruschi C.V., Caldwell B., Capuano V., Carter N.M.,
RA   Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A.,
RA   Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T.,
RA   Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D.,
RA   Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N.,
RA   Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G.,
RA   Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A.,
RA   Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M.,
RA   Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M.,
RA   Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S.,
RA   Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G.,
RA   Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B.,
RA   Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R.,
RA   Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P.,
RA   Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H.,
RA   Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P.,
RA   Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F.,
RA   Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H.,
RA   Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [4]
RP   GTP- AND GDP-BINDING, PROTEIN LEVELS, AND DISRUPTION PHENOTYPE.
RC   STRAIN=CRK6000;
RX   PubMed=12427945;
RA   Morimoto T., Loh P.C., Hirai T., Asai K., Kobayashi K., Moriya S.,
RA   Ogasawara N.;
RT   "Six GTP-binding proteins of the Era/Obg family are essential for cell
RT   growth in Bacillus subtilis.";
RL   Microbiology 148:3539-3552(2002).
CC   -!- FUNCTION: Necessary for normal cell division and for the
CC       maintenance of normal septation (By similarity).
CC   -!- FUNCTION: Binds GTP and GDP.
CC   -!- DISRUPTION PHENOTYPE: Essential for growth, it cannot be
CC       disrupted. In depletion experiments cells become over 3-fold
CC       longer, are abnormally curved and nucleoids condense.
CC   -!- MISCELLANEOUS: Estimated to be present at 1000 copies per cell.
CC   -!- SIMILARITY: Belongs to the EngB family.
CC   -!- SIMILARITY: Contains 1 G (guanine nucleotide-binding) domain.
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DR   EMBL; X76424; CAA53985.1; -; Genomic_DNA.
DR   EMBL; Z75208; CAA99541.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB14779.1; -; Genomic_DNA.
DR   PIR; I40422; I40422.
DR   RefSeq; NP_390697.1; NC_000964.3.
DR   PDB; 1SUL; X-ray; 2.00 A; A/B=1-195.
DR   PDB; 1SVI; X-ray; 1.95 A; A=1-195.
DR   PDB; 1SVW; X-ray; 2.80 A; A/B=1-195.
DR   PDBsum; 1SUL; -.
DR   PDBsum; 1SVI; -.
DR   PDBsum; 1SVW; -.
DR   ProteinModelPortal; P38424; -.
DR   SMR; P38424; 1-195.
DR   EnsemblBacteria; EBBACT00000000758; EBBACP00000000758; EBBACG00000000756.
DR   GeneID; 938009; -.
DR   GenomeReviews; AL009126_GR; BSU28190.
DR   KEGG; bsu:BSU28190; -.
DR   NMPDR; fig|224308.1.peg.2822; -.
DR   PATRIC; 18977514; VBIBacSub10457_2946.
DR   GenoList; BSU28190; -.
DR   GeneTree; EBGT00050000000579; -.
DR   HOGENOM; HBG447097; -.
DR   OMA; DLEFMEW; -.
DR   PhylomeDB; P38424; -.
DR   ProtClustDB; PRK00454; -.
DR   BioCyc; BSUB:BSU28190-MONOMER; -.
DR   GO; GO:0005622; C:intracellular; IEA:InterPro.
DR   GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR   GO; GO:0000917; P:barrier septum formation; IEA:UniProtKB-KW.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   HAMAP; MF_00321; GTPase_EngB; 1; -.
DR   InterPro; IPR019987; GTP-bd_ribosome_bio_YsxC.
DR   InterPro; IPR006073; GTP_binding_domain.
DR   InterPro; IPR005225; Small_GTP-bd_dom.
DR   KO; K03978; -.
DR   Pfam; PF01926; MMR_HSR1; 1.
DR   TIGRFAMs; TIGR03598; GTPase_YsxC; 1.
DR   TIGRFAMs; TIGR00231; Small_GTP; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cell cycle; Cell division; Complete proteome;
KW   GTP-binding; Nucleotide-binding; Reference proteome; Septation.
FT   CHAIN         1    195       Probable GTP-binding protein EngB.
FT                                /FTId=PRO_0000157736.
FT   DOMAIN       35    143       G.
FT   NP_BIND      30     37       GTP (Potential).
FT   NP_BIND      75     79       GTP (Potential).
FT   NP_BIND     142    145       GTP (Potential).
FT   STRAND        6     14
FT   HELIX        15     17
FT   STRAND       25     31
FT   HELIX        33     43
FT   STRAND       62     67
FT   TURN         68     70
FT   STRAND       71     75
FT   HELIX        86    102
FT   STRAND      106    114
FT   HELIX       121    132
FT   STRAND      137    142
FT   HELIX       144    146
FT   HELIX       149    151
FT   HELIX       152    163
FT   STRAND      169    173
FT   TURN        176    178
FT   HELIX       182    193
SQ   SEQUENCE   195 AA;  22026 MW;  12EC561C1806FFE3 CRC64;
     MKVTKSEIVI SAVKPEQYPE GGLPEIALAG RSNVGKSSFI NSLINRKNLA RTSSKPGKTQ
     TLNFYIINDE LHFVDVPGYG FAKVSKSERE AWGRMIETYI TTREELKAVV QIVDLRHAPS
     NDDVQMYEFL KYYGIPVIVI ATKADKIPKG KWDKHAKVVR QTLNIDPEDE LILFSSETKK
     GKDEAWGAIK KMINR
//