Running MakePangenome.. Information about input data: Number of genomes: 4 Number of sequences: 4 Number of sequences per genome: 1 Total length of input (bp): 6657863 Minimum genome length (bp): 1615199 Median genome length (bp): 1680863 Average genome length (bp): 1664465 Maximum genome length (bp): 1680937 ============================ [2021-Nov-15 22:10:15 UTC+03:00] BeginIteration 1, AnchorJoinerFast npge PostProcessing Length of all major blocks of at least 2 fragments: 111153 Stable blocks (represented in all genomes once, but not minor): fragments: 576 blocks: 144 Block identity: min: 0.8977 median: 0.9415 avg: 0.9382 max: 0.9763 Identity of joined blocks: 0.949734 Nucleotides in blocks: 352024 The percentage of input length: 5.28% ============================ [2021-Nov-15 22:11:01 UTC+03:00] BeginIteration 2, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 1399127 Stable blocks (represented in all genomes once, but not minor): fragments: 7364 blocks: 1841 Block identity: min: 0.8425 median: 0.9464 avg: 0.9436 max: 1 Identity of joined blocks: 0.951769 Nucleotides in blocks: 5272933 The percentage of input length: 79.19% Distance from previous pre-pangenome (bp): 4991060 The percentage of input length: 74.96% Distance is sufficient to carry on ============================ [2021-Nov-15 22:12:22 UTC+03:00] BeginIteration 3, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 1596351 Stable blocks (represented in all genomes once, but not minor): fragments: 7924 blocks: 1981 Block identity: min: 0.8425 median: 0.9462 avg: 0.9435 max: 1 Identity of joined blocks: 0.951753 Nucleotides in blocks: 5622045 The percentage of input length: 84.44% Distance from previous pre-pangenome (bp): 596549 The percentage of input length: 8.96% Distance is sufficient to carry on ============================ [2021-Nov-15 22:13:14 UTC+03:00] BeginIteration 4, AnchorJoinerFast qnpge Length of all major blocks of at least 2 fragments: 1605759 Stable blocks (represented in all genomes once, but not minor): fragments: 7924 blocks: 1981 Block identity: min: 0.8425 median: 0.9462 avg: 0.9435 max: 1 Identity of joined blocks: 0.951754 Nucleotides in blocks: 5622489 The percentage of input length: 84.44% Distance from previous pre-pangenome (bp): 29783 The percentage of input length: 0.45% Distance is sufficient to carry on ============================ [2021-Nov-15 22:14:03 UTC+03:00] BeginIteration 5, AnchorJoiner Length of all major blocks of at least 2 fragments: 1617329 Stable blocks (represented in all genomes once, but not minor): fragments: 7956 blocks: 1989 Block identity: min: 0.8425 median: 0.9462 avg: 0.9434 max: 1 Identity of joined blocks: 0.951726 Nucleotides in blocks: 5625490 The percentage of input length: 84.49% Distance from previous pre-pangenome (bp): 31920 The percentage of input length: 0.48% Distance is sufficient to carry on ============================ [2021-Nov-15 22:15:46 UTC+03:00] BeginIteration 6, AnchorJoiner Length of all major blocks of at least 2 fragments: 1617187 Stable blocks (represented in all genomes once, but not minor): fragments: 7944 blocks: 1986 Block identity: min: 0.8425 median: 0.9462 avg: 0.9434 max: 1 Identity of joined blocks: 0.951731 Nucleotides in blocks: 5624259 The percentage of input length: 84.47% Distance from previous pre-pangenome (bp): 1582 The percentage of input length: 0.02% Distance is too low => stopping this loop ============================ [2021-Nov-15 22:16:36 UTC+03:00] BeginIteration 7, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 1617598 Stable blocks (represented in all genomes once, but not minor): fragments: 7960 blocks: 1990 Block identity: min: 0.8468 median: 0.9461 avg: 0.9433 max: 1 Identity of joined blocks: 0.951707 Nucleotides in blocks: 5626561 The percentage of input length: 84.51% Distance from previous pre-pangenome (bp): 3356 The percentage of input length: 0.05% Distance is too low => stopping this loop ============================ [2021-Nov-15 22:19:39 UTC+03:00] StopInfo The pangenome has been constructed. Blocks' lengths: 1781396 major: 1772231 minor: 9165 ============================ All major blocks of at least 2 fragments: fragments: 3507 blocks: 879 fragments / blocks: 3.9897 Block identity: min: 0.8714 median: 0.9548 avg: 0.963 max: 1 Identity of joined blocks: 0.95187 Block sizes: min: 2 median: 4 avg: 3.99 max: 31 Fragment lengths: min: 83 median: 313 avg: 1855.71 max: 40596 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0037 max: 0.2441 GC content: min: 0.1726 median: 0.3804 avg: 0.3693 max: 0.5335 Nucleotides in blocks: 6507968 The percentage of input length: 97.74% Total length of blocks: 1646540 The percentage of total blocks' length: 92.42% ============================ Stable blocks (represented in all genomes once, but not minor): fragments: 1624 blocks: 406 fragments / blocks: 4 Block identity: min: 0.8793 median: 0.9424 avg: 0.9388 max: 0.9911 Identity of joined blocks: 0.946011 Block sizes: 4 Fragment lengths: min: 98 median: 1858 avg: 3530.41 max: 40596 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0.001 avg: 0.0044 max: 0.1359 GC content: min: 0.1902 median: 0.3911 avg: 0.3841 max: 0.5291 Nucleotides in blocks: 5733387 The percentage of input length: 86.11% Total length of blocks: 1437621 The percentage of total blocks' length: 80.7% ============================ Partial blocks (represented once in subset of genomes): fragments: 1044 blocks: 348 fragments / blocks: 3 Block identity: min: 0.9797 median: 1 avg: 0.9997 max: 1 Identity of joined blocks: 0.999908 Block sizes: 3 Fragment lengths: min: 100 median: 185 avg: 492.77 max: 10923 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0003 max: 0.0298 GC content: min: 0.1726 median: 0.3504 avg: 0.3494 max: 0.52 Nucleotides in blocks: 514450 The percentage of input length: 7.72% Total length of blocks: 171495 The percentage of total blocks' length: 9.62% ============================ Blocks with repeats (at least two copies in at least one genome): fragments: 839 blocks: 125 fragments / blocks: 6.712 Block identity: min: 0.8714 median: 0.9295 avg: 0.9391 max: 1 Identity of joined blocks: 0.956793 Block sizes: min: 2 median: 6 avg: 6.71 max: 31 Fragment lengths: min: 83 median: 139 avg: 310.05 max: 3634 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0108 max: 0.2441 GC content: min: 0.2008 median: 0.3761 avg: 0.3764 max: 0.5335 Nucleotides in blocks: 260131 The percentage of input length: 3.9% Total length of blocks: 37424 The percentage of total blocks' length: 2.1% ============================ Unique (blocks of 1 fragment but not minor): fragments: 347 blocks: 347 Fragment lengths: min: 100 median: 201 avg: 362.22 max: 4284 GC content: min: 0.1965 median: 0.3571 avg: 0.3579 max: 0.5774 Nucleotides in blocks: 125691 The percentage of input length: 1.88% Total length of blocks: 125691 The percentage of total blocks' length: 7.05% ============================ Minor blocks (too short to say smth about): fragments: 662 blocks: 222 fragments / blocks: 2.9819 Block identity: min: 0 median: 1 avg: 0.8424 max: 1 Identity of joined blocks: 0.942707 Block sizes: min: 1 median: 3 avg: 2.98 max: 22 Fragment lengths: min: 1 median: 26 avg: 36.56 max: 99 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0417 max: 3 GC content: min: 0 median: 0.3629 avg: 0.3821 max: 1 Nucleotides in blocks: 24204 The percentage of input length: 0.36% Total length of blocks: 9165 The percentage of total blocks' length: 0.51% The pangenome has been prepared for the next step: PostProcessing .. done