1: Proteomics. 2007 Jan;7(1):130-42.

An investigation of the effects of endometriosis on the proteome of human
eutopic endometrium: a heterogeneous tissue with a complex disease.

Fowler PA, Tattum J, Bhattacharya S, Klonisch T, Hombach-Klonisch S, Gazvani R,
Lea RG, Miller I, Simpson WG, Cash P.

Department of Obstetrics & Gynaecology, University of Aberdeen, Aberdeen, UK.
p.a.fowler@abdn.ac.uk

The pathogenesis of endometriosis includes the proliferation of heterogeneous
endometrial cells and their invasion into ectopic sites within the peritoneal
cavity. This may be due to abnormalities of the eutopic endometrium itself,
predisposing the cells to survive and implant ectopically. We investigated the
applicability of 2-DE gels and peptide mass mapping to identify candidate
endometrial proteins with a role in endometriosis. Despite the heterogeneous
nature of endometrium, our results show that combining the analysis of 2-DE gels
and peptide mass mapping yields consistent data. We identified dysregulated
proteins in women with endometriosis which included: (i) molecular chaperones
including heat shock protein 90 and annexin A2, (ii) proteins involved in
cellular redox state, such as peroxiredoxin 2, (iii) proteins involved in
protein and DNA formation/breakdown, including ribonucleoside-diphosphate
reductase, prohibitin and prolyl 4-hydroxylase, and (iv) secreted proteins, such
as apolipoprotein A1. These proteins have functions which suggest that they
could play a role in the pathogenesis of endometriosis. This study demonstrated
that 2-DE gel analysis and mass spectroscopic protein identification are
suitable for the identification of proteins with candidate associations with
endometriosis. These techniques should be used on a larger scale to identify
endometriosis-related proteins, thus improving the understanding of this complex
disease.

PMID: 17124717 [PubMed - in process]

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