PMID- 9851930
OWN - NLM
STAT- MEDLINE
DA  - 19981224
DCOM- 19981224
LR  - 20070319
IS  - 0036-8075 (Print)
IS  - 0036-8075 (Linking)
VI  - 282
IP  - 5396
DP  - 1998 Dec 11
TI  - Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene.
PG  - 2085-8
AB  - Mutations of the gene Lps selectively impede lipopolysaccharide (LPS) signal
      transduction in C3H/HeJ and C57BL/10ScCr mice, rendering them resistant to
      endotoxin yet highly susceptible to Gram-negative infection. The codominant Lpsd 
      allele of C3H/HeJ mice was shown to correspond to a missense mutation in the
      third exon of the Toll-like receptor-4 gene (Tlr4), predicted to replace proline 
      with histidine at position 712 of the polypeptide chain. C57BL/10ScCr mice are
      homozygous for a null mutation of Tlr4. Thus, the mammalian Tlr4 protein has been
      adapted primarily to subserve the recognition of LPS and presumably transduces
      the LPS signal across the plasma membrane. Destructive mutations of Tlr4
      predispose to the development of Gram-negative sepsis, leaving most aspects of
      immune function intact.
FAU - Poltorak, A
AU  - Poltorak A
AD  - Howard Hughes Medical Institute and the Department of Internal Medicine,
      University of Texas Southwestern Medical Center, Dallas, TX 75235-9050, USA.
FAU - He, X
AU  - He X
FAU - Smirnova, I
AU  - Smirnova I
FAU - Liu, M Y
AU  - Liu MY
FAU - Van Huffel, C
AU  - Van Huffel C
FAU - Du, X
AU  - Du X
FAU - Birdwell, D
AU  - Birdwell D
FAU - Alejos, E
AU  - Alejos E
FAU - Silva, M
AU  - Silva M
FAU - Galanos, C
AU  - Galanos C
FAU - Freudenberg, M
AU  - Freudenberg M
FAU - Ricciardi-Castagnoli, P
AU  - Ricciardi-Castagnoli P
FAU - Layton, B
AU  - Layton B
FAU - Beutler, B
AU  - Beutler B
LA  - eng
SI  - GENBANK/AF095353
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - UNITED STATES
TA  - Science
JT  - Science (New York, N.Y.)
JID - 0404511
RN  - 0 (Drosophila Proteins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Chromosome Mapping
MH  - Cloning, Molecular
MH  - *Drosophila Proteins
MH  - Genes, Dominant
MH  - Gram-Negative Bacterial Infections/immunology
MH  - Homozygote
MH  - Lipopolysaccharides/*metabolism/pharmacology
MH  - Macrophages/metabolism
MH  - Membrane Glycoproteins/chemistry/*genetics/metabolism
MH  - Mice
MH  - Mice, Inbred C3H
MH  - Mice, Inbred C57BL
MH  - Molecular Sequence Data
MH  - Mutation, Missense
MH  - Point Mutation
MH  - RNA, Messenger/genetics/metabolism
MH  - Receptors, Cell Surface/chemistry/*genetics/metabolism
MH  - *Signal Transduction
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 1998/12/16
MHDA- 1998/12/16 00:01
CRDT- 1998/12/16 00:00
PST - ppublish
SO  - Science. 1998 Dec 11;282(5396):2085-8.