PMID- 3871837
OWN - NLM
STAT- MEDLINE
DA  - 19850404
DCOM- 19850404
LR  - 20130929
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 161
IP  - 3
DP  - 1985 Mar 1
TI  - Murine epidermal Langerhans cells mature into potent immunostimulatory dendritic 
      cells in vitro.
PG  - 526-46
AB  - Murine epidermal Langerhans cells (LC) have been studied in tissue culture and
      compared to spleen dendritic cells (DC). LC comprised 3% of the starting cell
      suspensions and were distinguished from keratinocytes by cytology and reactivity 
      with anti-Ia and anti-Mac-1 monoclonal antibodies. The LC were nonadherent, had a
      low buoyant density, did not proliferate, and could be enriched to 10-50% purity.
      LC continued to exhibit Ia and Mac-1 antigens for 4 d in culture. However, LC
      rapidly lost Birbeck granules, Fc receptors, F4/80 antigen, and cytochemical
      reactivity for nonspecific esterase and membrane ATPase. As a result, the
      ultrastructure and phenotype of cultured LC became remarkably similar to lymphoid
      DC. Stimulatory capacity for T cell proliferative responses (oxidative
      mitogenesis and the mixed leukocyte reaction) was monitored daily. Initially,
      stimulatory capacity was very weak, even though LC expressed substantial levels
      of Ia antigens. After 2-3 d in culture, LC had become 3-10 times more potent than
      spleen DC. 30 LC could induce significant responses in cultures of 3 X 10(5)
      responding T cells. Removal of Ia+ LC at the start of culture ablated the
      development of stimulatory activity, but exposure to 1,500 rad of ionizing
      irradiation did not. Mixing experiments showed that contaminating Ia- epidermal
      cells did not alter the function of Ia+ stimulators. Therefore, LC seem to be
      immunologically immature, but acquire many of the features of spleen DC during
      culture. We suggest that functioning lymphoid DC may, in general, be derived from
      less mature precursors located in nonlymphoid tissues.
FAU - Schuler, G
AU  - Schuler G
FAU - Steinman, R M
AU  - Steinman RM
LA  - eng
GR  - AI 13013/AI/NIAID NIH HHS/United States
GR  - CA 30198/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - UNITED STATES
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Histocompatibility Antigens Class II)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/diagnostic use
MH  - Antigen-Antibody Reactions
MH  - Antigen-Presenting Cells/*cytology/immunology/ultrastructure
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Epidermis/ultrastructure
MH  - Female
MH  - Histocompatibility Antigens Class II/analysis
MH  - Histocytochemistry
MH  - Langerhans Cells/*cytology/immunology/ultrastructure
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred ICR
MH  - Phenotype
MH  - Spleen/cytology
MH  - T-Lymphocytes/immunology
PMC - PMC2187584
OID - NLM: PMC2187584
EDAT- 1985/03/01
MHDA- 1985/03/01 00:01
CRDT- 1985/03/01 00:00
PST - ppublish
SO  - J Exp Med. 1985 Mar 1;161(3):526-46.