FastQCFastQC Report
Чт 19 фев 2015
Ath_tae_CTTGTA_L003_R2_001_clean.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAth_tae_CTTGTA_L003_R2_001_clean.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3892659
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC6251.7389539E-912.0012419
CGCCGTA6000.011.7393446-47
GCGGCCG2859.342184E-710.69237626-27
GGCCGCG2351.6879229E-49.97494828-29
TGGTCGC6450.09.44925942-43
GTCGCCG7500.09.38012644-45
AGCGTCG6850.09.23909510-11
CGGCCGC3052.4196856E-59.22161324-25
GTACTGG6758.044553E-69.0332371
CGTCGTG7700.08.82802312-13
CGCGGCC3002.0373301E-48.59403224-25
GGTCGCC4851.5312398E-67.733255442-43
CCGTATC8801.8189894E-127.480210348-49
TCGGTGG9850.07.1399838-39
AGAGCGT11101.1453943E-56.75745538
GTATGGC8600.00127842986.5446532
TATCGCG4450.00189546686.32039122-23
TCGCGGC4150.0063871036.21255324-25
CGCGTCT4650.00309872816.04940142-43
CTCGGTG9156.0982165E-75.63655338-39
Produced by FastQC (version 0.11.2)