REM -------------------------------------------------------------------- 3OES REM 3OES REM STRIDE: Knowledge-based secondary structure assignment 3OES REM Please cite: D.Frishman & P.Argos, Proteins XX, XXX-XXX, 1995 3OES REM 3OES REM Residue accessible surface area calculation 3OES REM Please cite: F.Eisenhaber & P.Argos, J.Comp.Chem. 14, 1272-1280, 1993 3OES REM F.Eisenhaber et al., J.Comp.Chem., 1994, submitted 3OES REM 3OES REM ------------------------ General information ----------------------- 3OES REM 3OES HDR HYDROLASE 13-AUG-10 3OES 3OES CMP MOL_ID: 1; 3OES CMP MOLECULE: GTPASE RHEBL1; 3OES CMP CHAIN: A; 3OES CMP SYNONYM: RAS HOMOLOG ENRICHED IN BRAIN-LIKE PROTEIN 1, RHEB- 3OES CMP PROTEIN 1, RHEB2, RAS HOMOLOG ENRICHED IN BRAIN LIKE-1 C, RH 3OES CMP ENGINEERED: YES 3OES SRC MOL_ID: 1; 3OES SRC ORGANISM_SCIENTIFIC: HOMO SAPIENS; 3OES SRC ORGANISM_COMMON: HUMAN; 3OES SRC ORGANISM_TAXID: 9606; 3OES SRC GENE: RHEBL1; 3OES SRC EXPRESSION_SYSTEM: ESCHERICHIA COLI; 3OES SRC EXPRESSION_SYSTEM_TAXID: 562; 3OES SRC EXPRESSION_SYSTEM_STRAIN: BL21-V2R-PRARE2; 3OES SRC EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID; 3OES SRC EXPRESSION_SYSTEM_PLASMID: PET28-MHL 3OES AUT L.NEDYALKOVA,N.ZHONG,W.TEMPEL,Y.TONG,L.SHEN,P.LOPPNAU,C.H.ARR 3OES AUT A.M.EDWARDS,C.BOUNTRA,J.WEIGELT,A.BOCHKAREV,H.PARK,STRUCTURA 3OES AUT GENOMICS CONSORTIUM (SGC) 3OES REM 3OES REM -------------------- Secondary structure summary ------------------- 3OES REM 3OES CHN /home/proj/stride/tmp/tmpZe7b0_pdb A 3OES REM 3OES REM . . . . . 3OES SEQ 1 RYRKVVILGYRCVGKTSLAHQFVEGEFSEGYDPTVENTYSKIVTEFHLHL 50 3OES STR EEEEEEE TTTT HHHHHHHHHH TTEEEEEEEETTEEEEE 3OES REM 3OES REM . . . . . 3OES SEQ 51 VDTAGQDEYSILPYSFIIGVHGYVLVYSVTSLHSFQVIESLYQKLHERVP 100 3OES STR EEE TTTT GGGTTTT EEEEEEETTTHHHHHHHHHHHHHHHH 3OES REM 3OES REM . . . . . 3OES SEQ 101 VVLVGNKADLSPEREVQAVEGKKLAESWGATFMESSARENQLTQGIFTKV 150 3OES STR EEEEEETTTTGGG HHHHHHHHHHH EEEE TTTTHHHHHHHHHHH 3OES REM 3OES REM 3OES SEQ 151 IQEIARV 157 3OES STR HHHHHH 3OES REM 3OES REM 3OES REM 3OES LOC AlphaHelix LYS 19 A GLU 28 A 3OES LOC AlphaHelix LEU 90 A GLU 105 A 3OES LOC AlphaHelix ALA 131 A TRP 141 A 3OES LOC AlphaHelix ASN 153 A ARG 169 A 3OES LOC 310Helix TYR 72 A PHE 74 A 3OES LOC 310Helix SER 124 A GLU 126 A 3OES LOC Strand TYR 6 A LEU 12 A 3OES LOC Strand GLU 40 A VAL 47 A 3OES LOC Strand PHE 54 A THR 61 A 3OES LOC Strand GLY 80 A SER 86 A 3OES LOC Strand VAL 114 A ASN 119 A 3OES LOC Strand THR 144 A GLU 147 A 3OES LOC TurnII TYR 14 A VAL 17 A 3OES LOC TurnIV THR 38 A ASN 41 A 3OES LOC TurnIV ILE 46 A GLU 53 A 3OES LOC TurnIV VAL 47 A PHE 54 A 3OES LOC TurnI ASP 65 A SER 68 A 3OES LOC TurnII ILE 75 A VAL 78 A 3OES LOC TurnI SER 86 A SER 89 A 3OES LOC TurnVIII VAL 87 A LEU 90 A 3OES LOC TurnI LYS 120 A LEU 123 A 3OES LOC TurnIV ALA 121 A SER 124 A 3OES LOC TurnI SER 149 A GLU 152 A 3OES LOC TurnVIII ALA 150 A ASN 153 A 3OES REM 3OES REM --------------- Detailed secondary structure assignment------------- 3OES REM 3OES REM |---Residue---| |--Structure--| |-Phi-| |-Psi-| |-Area-| 3OES ASG ARG A 5 1 C Coil 360.00 112.08 86.0 3OES ASG TYR A 6 2 E Strand -77.96 122.95 111.8 3OES ASG ARG A 7 3 E Strand -136.44 135.90 68.9 3OES ASG LYS A 8 4 E Strand -103.36 122.27 51.1 3OES ASG VAL A 9 5 E Strand -120.05 132.70 0.0 3OES ASG VAL A 10 6 E Strand -105.04 126.53 0.0 3OES ASG ILE A 11 7 E Strand -96.38 122.17 0.6 3OES ASG LEU A 12 8 E Strand -122.15 148.82 0.0 3OES ASG GLY A 13 9 C Coil 173.74 174.84 0.0 3OES ASG TYR A 14 10 T Turn -56.71 162.10 38.1 3OES ASG ARG A 15 11 T Turn -72.85 149.50 111.8 3OES ASG CYS A 16 12 T Turn 72.57 10.04 68.0 3OES ASG VAL A 17 13 T Turn -78.84 -13.72 2.0 3OES ASG GLY A 18 14 C Coil 106.77 25.14 11.9 3OES ASG LYS A 19 15 H AlphaHelix -58.21 -59.57 11.4 3OES ASG THR A 20 16 H AlphaHelix -57.25 -42.93 37.7 3OES ASG SER A 21 17 H AlphaHelix -63.80 -39.30 36.2 3OES ASG LEU A 22 18 H AlphaHelix -57.78 -56.79 0.6 3OES ASG ALA A 23 19 H AlphaHelix -55.89 -43.34 0.0 3OES ASG HIS A 24 20 H AlphaHelix -67.57 -38.65 69.4 3OES ASG GLN A 25 21 H AlphaHelix -60.22 -53.90 11.2 3OES ASG PHE A 26 22 H AlphaHelix -54.23 -46.11 10.2 3OES ASG VAL A 27 23 H AlphaHelix -89.88 -43.80 22.7 3OES ASG GLU A 28 24 H AlphaHelix -102.89 -8.13 123.1 3OES ASG GLY A 29 25 C Coil 66.50 30.64 48.3 3OES ASG GLU A 30 26 C Coil -137.41 151.67 47.0 3OES ASG PHE A 31 27 C Coil -125.01 131.76 79.6 3OES ASG SER A 32 28 C Coil -106.84 133.91 78.8 3OES ASG GLU A 33 29 C Coil -78.30 -3.97 176.9 3OES ASG GLY A 34 30 C Coil -73.90 -178.98 51.8 3OES ASG TYR A 35 31 C Coil -147.18 129.97 150.6 3OES ASG ASP A 36 32 C Coil -141.30 112.03 133.4 3OES ASG PRO A 37 33 C Coil -61.17 129.39 78.0 3OES ASG THR A 38 34 T Turn -82.22 138.71 23.6 3OES ASG VAL A 39 35 T Turn -95.08 -62.86 131.3 3OES ASG GLU A 40 36 E Strand -168.94 125.53 96.0 3OES ASG ASN A 41 37 E Strand -114.44 149.96 55.5 3OES ASG THR A 42 38 E Strand -123.91 135.97 80.1 3OES ASG TYR A 43 39 E Strand -120.40 122.44 33.0 3OES ASG SER A 44 40 E Strand -106.38 133.08 61.9 3OES ASG LYS A 45 41 E Strand -145.78 145.26 72.2 3OES ASG ILE A 46 42 E Strand -94.46 114.29 114.6 3OES ASG VAL A 47 43 E Strand -106.12 124.18 16.1 3OES ASG THR A 48 44 T Turn -88.11 360.00 131.9 3OES ASG GLU A 53 45 T Turn 360.00 103.69 113.8 3OES ASG PHE A 54 46 E Strand -113.60 134.66 44.3 3OES ASG HIS A 55 47 E Strand -96.49 106.27 76.9 3OES ASG LEU A 56 48 E Strand -93.91 123.59 0.6 3OES ASG HIS A 57 49 E Strand -106.91 108.67 35.3 3OES ASG LEU A 58 50 E Strand -92.91 116.12 0.6 3OES ASG VAL A 59 51 E Strand -92.73 106.46 11.2 3OES ASG ASP A 60 52 E Strand -92.68 109.06 0.0 3OES ASG THR A 61 53 E Strand -93.53 159.91 0.0 3OES ASG ALA A 62 54 C Coil -60.12 135.65 32.4 3OES ASG GLY A 63 55 C Coil -53.27 146.18 25.9 3OES ASG GLN A 64 56 C Coil -135.25 158.04 24.6 3OES ASG ASP A 65 57 T Turn -124.92 -174.48 43.8 3OES ASG GLU A 66 58 T Turn -63.08 -20.63 62.3 3OES ASG TYR A 67 59 T Turn -95.87 -12.68 181.3 3OES ASG SER A 68 60 T Turn -66.87 151.89 22.8 3OES ASG ILE A 69 61 C Coil -112.73 116.88 153.5 3OES ASG LEU A 70 62 C Coil -74.16 128.60 36.0 3OES ASG PRO A 71 63 C Coil -76.00 161.79 58.4 3OES ASG TYR A 72 64 G 310Helix -51.68 -34.71 75.9 3OES ASG SER A 73 65 G 310Helix -53.15 -28.36 62.9 3OES ASG PHE A 74 66 G 310Helix -82.33 -21.97 11.0 3OES ASG ILE A 75 67 T Turn -101.35 -48.84 68.2 3OES ASG ILE A 76 68 T Turn -74.57 136.53 63.1 3OES ASG GLY A 77 69 T Turn 70.52 5.46 53.8 3OES ASG VAL A 78 70 T Turn -87.87 116.23 18.8 3OES ASG HIS A 79 71 C Coil -81.05 -18.46 66.0 3OES ASG GLY A 80 72 E Strand -172.13 158.25 0.0 3OES ASG TYR A 81 73 E Strand -125.61 142.62 20.0 3OES ASG VAL A 82 74 E Strand -113.57 112.13 0.4 3OES ASG LEU A 83 75 E Strand -97.34 118.72 0.0 3OES ASG VAL A 84 76 E Strand -110.93 132.58 0.0 3OES ASG TYR A 85 77 E Strand -124.33 176.00 0.0 3OES ASG SER A 86 78 E Strand -111.17 132.96 3.2 3OES ASG VAL A 87 79 T Turn -67.20 -22.96 0.0 3OES ASG THR A 88 80 T Turn -87.12 5.70 23.6 3OES ASG SER A 89 81 T Turn -141.77 101.23 31.1 3OES ASG LEU A 90 82 H AlphaHelix -59.50 -39.34 63.3 3OES ASG HIS A 91 83 H AlphaHelix -53.05 -44.06 74.4 3OES ASG SER A 92 84 H AlphaHelix -65.02 -35.23 0.4 3OES ASG PHE A 93 85 H AlphaHelix -67.82 -35.79 7.6 3OES ASG GLN A 94 86 H AlphaHelix -67.21 -44.10 99.8 3OES ASG VAL A 95 87 H AlphaHelix -61.45 -38.60 1.6 3OES ASG ILE A 96 88 H AlphaHelix -61.10 -32.72 0.0 3OES ASG GLU A 97 89 H AlphaHelix -61.39 -49.22 80.8 3OES ASG SER A 98 90 H AlphaHelix -53.78 -51.45 40.5 3OES ASG LEU A 99 91 H AlphaHelix -58.05 -38.48 0.0 3OES ASG TYR A 100 92 H AlphaHelix -63.05 -51.21 43.3 3OES ASG GLN A 101 93 H AlphaHelix -52.14 -50.33 38.7 3OES ASG LYS A 102 94 H AlphaHelix -51.92 -37.22 55.4 3OES ASG LEU A 103 95 H AlphaHelix -66.14 -45.24 38.9 3OES ASG HIS A 104 96 H AlphaHelix -73.48 -29.73 96.5 3OES ASG GLU A 105 97 H AlphaHelix -72.21 360.00 181.6 3OES ASG ARG A 111 98 C Coil 360.00 137.41 180.6 3OES ASG VAL A 112 99 C Coil -121.95 155.88 31.0 3OES ASG PRO A 113 100 C Coil -57.02 135.16 18.5 3OES ASG VAL A 114 101 E Strand -129.40 127.85 11.0 3OES ASG VAL A 115 102 E Strand -108.74 124.77 0.0 3OES ASG LEU A 116 103 E Strand -99.65 125.46 0.0 3OES ASG VAL A 117 104 E Strand -123.28 117.03 0.0 3OES ASG GLY A 118 105 E Strand -90.22 111.02 0.0 3OES ASG ASN A 119 106 E Strand -96.39 174.42 3.8 3OES ASG LYS A 120 107 T Turn 62.05 31.08 61.4 3OES ASG ALA A 121 108 T Turn -61.51 -16.06 24.8 3OES ASG ASP A 122 109 T Turn -63.91 -27.20 53.7 3OES ASG LEU A 123 110 T Turn -88.91 60.84 54.4 3OES ASG SER A 124 111 G 310Helix -58.91 -46.21 78.2 3OES ASG PRO A 125 112 G 310Helix -61.02 -15.38 121.8 3OES ASG GLU A 126 113 G 310Helix -108.66 5.18 108.6 3OES ASG ARG A 127 114 C Coil -58.10 134.66 77.9 3OES ASG GLU A 128 115 C Coil -118.88 -20.31 118.1 3OES ASG VAL A 129 116 C Coil -119.70 119.75 0.0 3OES ASG GLN A 130 117 C Coil -79.37 146.55 129.1 3OES ASG ALA A 131 118 H AlphaHelix -49.04 -46.77 41.1 3OES ASG VAL A 132 119 H AlphaHelix -58.09 -27.18 108.8 3OES ASG GLU A 133 120 H AlphaHelix -73.91 -48.50 82.8 3OES ASG GLY A 134 121 H AlphaHelix -68.78 -36.51 0.0 3OES ASG LYS A 135 122 H AlphaHelix -57.79 -44.99 40.4 3OES ASG LYS A 136 123 H AlphaHelix -64.95 -42.83 123.2 3OES ASG LEU A 137 124 H AlphaHelix -55.57 -54.70 21.3 3OES ASG ALA A 138 125 H AlphaHelix -53.90 -46.23 1.2 3OES ASG GLU A 139 126 H AlphaHelix -56.40 -43.79 106.4 3OES ASG SER A 140 127 H AlphaHelix -59.49 -22.82 86.3 3OES ASG TRP A 141 128 H AlphaHelix -97.36 -14.87 42.8 3OES ASG GLY A 142 129 C Coil 77.33 29.35 68.4 3OES ASG ALA A 143 130 C Coil -106.20 159.51 15.5 3OES ASG THR A 144 131 E Strand -88.04 144.84 45.1 3OES ASG PHE A 145 132 E Strand -121.63 141.47 44.5 3OES ASG MET A 146 133 E Strand -156.53 166.50 41.9 3OES ASG GLU A 147 134 E Strand -113.60 157.94 49.7 3OES ASG SER A 148 135 C Coil -154.09 174.63 0.2 3OES ASG SER A 149 136 T Turn -146.34 128.63 0.4 3OES ASG ALA A 150 137 T Turn -70.89 -10.27 4.0 3OES ASG ARG A 151 138 T Turn -79.88 -11.35 136.9 3OES ASG GLU A 152 139 T Turn -119.69 116.33 80.2 3OES ASG ASN A 153 140 H AlphaHelix -60.23 -37.14 59.9 3OES ASG GLN A 154 141 H AlphaHelix -58.34 -54.91 164.5 3OES ASG LEU A 155 142 H AlphaHelix -61.85 -34.02 82.3 3OES ASG THR A 156 143 H AlphaHelix -77.23 -43.04 0.0 3OES ASG GLN A 157 144 H AlphaHelix -49.46 -50.11 91.2 3OES ASG GLY A 158 145 H AlphaHelix -51.78 -34.76 28.0 3OES ASG ILE A 159 146 H AlphaHelix -58.84 -45.37 0.2 3OES ASG PHE A 160 147 H AlphaHelix -90.92 -35.51 0.4 3OES ASG THR A 161 148 H AlphaHelix -64.25 -37.25 61.1 3OES ASG LYS A 162 149 H AlphaHelix -60.18 -52.06 25.6 3OES ASG VAL A 163 150 H AlphaHelix -60.01 -29.84 0.0 3OES ASG ILE A 164 151 H AlphaHelix -75.46 -47.57 11.8 3OES ASG GLN A 165 152 H AlphaHelix -56.12 -38.03 84.1 3OES ASG GLU A 166 153 H AlphaHelix -71.36 -29.19 40.6 3OES ASG ILE A 167 154 H AlphaHelix -74.42 -45.40 19.6 3OES ASG ALA A 168 155 H AlphaHelix -58.81 -30.22 63.7 3OES ASG ARG A 169 156 H AlphaHelix -66.42 -67.35 118.8 3OES ASG VAL A 170 157 C Coil -77.52 360.00 106.5 3OES