{
 "cells": [
  {
   "cell_type": "code",
   "execution_count": 2,
   "id": "1a11fe6b-cbcb-4dcb-acaa-a67e2bfa82ad",
   "metadata": {},
   "outputs": [
    {
     "name": "stdout",
     "output_type": "stream",
     "text": [
      "Последовательность:  GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAGAUCUGGAGGUCUGUGGgUUCGAUCCCCGUAAUCUCCA\n",
      "Структура MFE:      ..((((((...((((((.((......)).))))))))))))((((((.((.(((......)))))..)))))). (-23.90 kcal/mol)\n",
      "Структура MEA:      ..((((((...((((((.((......)).))))))))))))((((((..(.(((......))))...)))))). (-22.90 kcal/mol)\n"
     ]
    }
   ],
   "source": [
    "import RNA\n",
    "\n",
    "# Ваша последовательность тРНК 1EHZ\n",
    "seq = \"GCGGAUUUAGCUCAGUUGGGAGAGCGCCAGACUGAAGAUCUGGAGGUCUGUGGgUUCGAUCCCCGUAAUCUCCA\"\n",
    "\n",
    "# Создание объекта для расчетов\n",
    "fc = RNA.fold_compound(seq)\n",
    "\n",
    "# Подсчет структуры с минимальной свободной энергией (MFE)\n",
    "(mfe_struct, mfe) = fc.mfe()\n",
    "\n",
    "# Считаем альтернативную структуру (MEA)\n",
    "fc.exp_params_rescale(mfe)\n",
    "(pp, pf) = fc.pf()\n",
    "(MEA_struct, MEA) = fc.MEA()\n",
    "MEA_en = fc.eval_structure(MEA_struct)\n",
    "\n",
    "# Выводим результаты на экран\n",
    "print(\"Последовательность: \", seq)\n",
    "print(\"Структура MFE:     \", mfe_struct, f\"({mfe:6.2f} kcal/mol)\")\n",
    "print(\"Структура MEA:     \", MEA_struct, f\"({MEA_en:6.2f} kcal/mol)\")\n"
   ]
  },
  {
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   "source": []
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