FastQCFastQC Report
Вт 4 дек 2018
SRR4240378.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240378.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4420587
Sequences flagged as poor quality0
Sequence length36
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA276190.6247812790473302No Hit
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA140210.31717507199835676Illumina Single End Adapter 1 (100% over 33bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN113360.2564365320714195No Hit
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN111160.25145981744053447No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN105280.23815841651798733No Hit
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTAGAT100330.22696080859849607Illumina Single End Adapter 1 (96% over 32bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAT97970.2216221510853649Illumina Single End Adapter 1 (100% over 33bp)
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN53310.12059484407839953No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCTC78450.028.9655158
GAGCTCG78950.028.6733639
CGGAAGA82450.028.5420364
TCGGAAG85050.028.5174473
GAAGAGC81800.027.9865196
GGAAGAG83600.027.8647635
AAGAGCT82400.027.7556557
GATCGGA89050.027.5367681
AGCTCGT80350.027.5131210
GCTCGTA80950.027.30855611
GCCGTCT76900.027.24230219
TGCCGTC77200.027.17698118
CGTATGC81050.027.1199414
ATCGGAA90700.026.9209122
GTATGCC82300.026.70757315
TCGTATG83000.026.59875913
CTCGTAT84050.026.57949312
TATGCCG81500.026.4675116
ATGCCGT79950.026.4261717
CCGTCTT80050.026.38109620
Produced by FastQC (version 0.11.2)