FastQCFastQC Report
Вт 4 дек 2018
reads_better.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamereads_better.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163033
Sequences flagged as poor quality0
Sequence length30-36
%GC28

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA185390.4453243584665315No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG1807.943472E-710.8275199
AGAGCTC2854.4507033E-88.9434358
CGGAAGA3603.3287506E-108.7601854
TCGGAAG4251.2187229E-108.1273923
AGATCGG7150.07.01866229
GCTCGTA2802.1071908E-46.95303911
GAGATCG3801.3280036E-56.690115530
GATCGGA7950.06.6270061
GGAAGAG5251.1768861E-86.5368095
ACAGATC4702.899433E-45.4090330
ATCGGAA9758.0035534E-115.23399542
Produced by FastQC (version 0.11.2)