Палочковидный вирус гибискуса GD1
Текстовое описание:
Hibiscus bacilliform virus GD1.
Систематика: Viruses->Retro-transcribing viruses->Caulimoviridae->Badnavirus.
Иллюстрация к полному геному вируса из банка NCBI представлена на рис.1:
Носителем генома является двуцепочечная РНК. Вирус относится к ретровирусам, то есть после попадания генома вируса в цитоплазму клетки-хозяина с помощью фермента ревертазы(обратной транскриптазы) на этой РНК как на матрице синтезируется одна цепь ДНК, на ней синтезируется комплементарная ей цепь ДНК, и готовая двуцепочечная ДНК встраивается в ДНК клетки хозяина во время её деления, когда этому не препятствует ядерная оболочка.
Геном вируса содержит четыре гена, по два на каждой из цепей РНК, сводная информация по которым представлена в таблице ниже:
| Метка гена | № первой НК | № последней НК | направление | id и название белка |
| BU35_gp1 | 257 | 688 | прямое (на цепи 1) | YP_009002583.1, hypothetical protein |
| BU35_gp2 | 688 | 1095 | обратное (на цепи 2) | YP_009002584.1, hypothetical protein |
| BU35_gp3 | 1092 | 6770 | прямое (на цепи 1) | YP_009002585.1, polyprotein |
| BU35_gp4 | 6416 | 6847 | обратное (на цепи 2) | YP_009002586.1, hypothetical protein |
Интересным представляется участок, где первый и второй гены пересекаются всего на один-единственный нуклеотид №688 (рис. 2):
Но если мои представления об интересном участке не совпадают с заданием, привожу начало и конец пересечения 3-его и 4-ого гена (рис. 3):
Информация из записей банка:
Идентификатор записи для Hibiscus bacilliform virus GD1:NC_023485
В файле содержится последовательность полного генома вируса длиной 7435 нуклеотидных пар.
Ниже приводится полное содержимое файла с описанием генома вируса.
LOCUS NC_023485 7435 bp DNA circular VRL 21-FEB-2014
DEFINITION Hibiscus bacilliform virus GD1, complete genome.
ACCESSION NC_023485
VERSION NC_023485.1 GI:586929335
DBLINK BioProject: PRJNA238954
KEYWORDS RefSeq.
SOURCE Hibiscus bacilliform virus GD1
ORGANISM Hibiscus bacilliform virus GD1
Viruses; Retro-transcribing viruses; Caulimoviridae; Badnavirus.
REFERENCE 1 (bases 1 to 7435)
AUTHORS Tang,Y.F. and He,Z.F.
TITLE Genomic characterisation of Hibiscus bacilliform virus
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7435)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (20-FEB-2014) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 7435)
AUTHORS Tang,Y.F. and He,Z.F.
TITLE Direct Submission
JOURNAL Submitted (19-NOV-2013) Plant Protection Research Institute,
Guangdong Academy of Agricultural Sciences, Jinying Rd No. 7,
Tianhe District, Guangzhou, Guangdong 510640, China
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to KF875586.
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..7435
/organism="Hibiscus bacilliform virus GD1"
/mol_type="genomic DNA"
/isolate="GD1"
/host="Hibiscus rosa-sinensis"
/db_xref="taxon:1459800"
/country="China: Guangdong"
/collection_date="03-Sep-2013"
/collected_by="Z.F.He"
gene 257..688
/locus_tag="BU35_gp1"
/db_xref="GeneID:18388309"
CDS 257..688
/locus_tag="BU35_gp1"
/note="ORF1"
/codon_start=1
/product="hypothetical protein"
/protein_id="YP_009002583.1"
/db_xref="GI:586929336"
/db_xref="GeneID:18388309"
/translation="MSRRWEEEIQKWYEQSSTSSLEYLDLASTSKVSNQQLAHNLSVI
YNHSSLSSRVQIKNYKKIQESLDSIESRISKLESNLKNLTKTFIANRPLTQAEVTTLV
SEISQQPKLVEAQALQLTEDLNKKLTRVEKLLHKLEQWSQS"
misc_feature 302..646
/locus_tag="BU35_gp1"
/note="Protein of unknown function (DUF1319); Region:
DUF1319; pfam07028"
/db_xref="CDD:254028"
gene 688..1095
/locus_tag="BU35_gp2"
/db_xref="GeneID:18388306"
CDS 688..1095
/locus_tag="BU35_gp2"
/note="ORF2"
/codon_start=1
/product="hypothetical protein"
/protein_id="YP_009002584.1"
/db_xref="GI:586929337"
/db_xref="GeneID:18388306"
/translation="MSHSRTRATASYKEAVAATEDIQPPAVGFAKPSDNVGAQSSSSV
IIKQNNTIIQLLASLSEKVNTLETEVRHIKNVFDSREPSTSTLPTDLIEKLEGLTLTD
KQERPKERKGTLFAFKDPYVILQEEKEKLKKAT"
gene 1092..6770
/locus_tag="BU35_gp3"
/db_xref="GeneID:18388307"
CDS 1092..6770
/locus_tag="BU35_gp3"
/note="ORF3"
/codon_start=1
/product="polyprotein"
/protein_id="YP_009002585.1"
/db_xref="GI:586929338"
/db_xref="GeneID:18388307"
/translation="MSTTTTQTVPPVTRNATRRQQGERETPLFEDQIRDYRNSQRRAY
TTQRAVRRLRRRITGRQPYQETLEQEVDPQADLQMSMQERARLVPAEVLYRSRRDTIH
HRVYSHRSEEAILCTDGNQVDRTFIQPESMEALQRTGISFIHIGIMQVRLQILHRQNE
GTMALVVFRDTRWNGDQSIFATMEVDLTEGRQIIYVVPDTMMTIGDFARNIQISILTR
GYSNWQNGEANLLVTRGIVGRLSNTPNVAFAYEIGNVTDYLTSQGVQAIAGRRYDSRE
LQNQQWILRPPQVERLPQAPKNVDTRNLIDGSISLRFRDYATTSTPELPSYNEQDEEV
DPDEEQLMEHVVAAFVEEEPKEIWDTLGQPSGKFDFLVKYSAPASSKIRIEDIVPTGW
GDEWEQPEQQQSPQQQQLLSCSQEESDDEDDWVSRFQFLAQFDEEPATQPQSPQSPTW
YNPFAEGGGSDENDENPDPEESEEMGNEMGYETEVEEQEEKVAMIQEDPDLLPYPHLR
ELLKNEEKVFSTQQSEVSSYRPPQDTAMTPAGYAPARGEASSSTHQPMFEGYMPRRPQ
FKQDRSTEYWQFPTAQGQTGAMFVIPRQIGLFHDAFNRWESITKNYVASQGFTDSKDK
AEFMENLLGETEKLTWVQWRMNFPTEYQEMVNAADGREGTQNILSQMRRVFTLEDPTT
GSTAVQDEAYRDLERLSCTNIKDIVSFLNDYARLAAKSGRMFISPELSEKLWLKMPYD
LGNKIRTAYEAKHEGNTIGVFPRILFTYKYLEQECKEAAFRRSLKNLSFCSEMPLPGY
YGEKKRYGVRRSTTYKGKPHPTHARIEKQKHLVRNKRCKCYLCGEMGHFARECPNDKK
NTRRVAMYEQLNIPEGFEVVSVEEGEEVSDAIFSISENEDEDALHTGGAFAETIFMFH
EGTDNVYWAGKKDGYLPYKRITREQFQCEHEWQMNQEVATVELEKCSFCKQETRRRMR
LHCPKCRITSCVLCSSNYCGIAIKPEPVQTSFFNEKSLLTQQQEYIAYQTAEIKKLQE
QLKQTEEKLAEAERKIRGYENMEVLEKERKEMALKDMQRKIDEEIKKAAMQELPTEKE
LWEQREGKAHIAGAVIAASTETGREERRPVKGNMLYNMDVEIDIPSVPRFTVKAILDT
GATSCCISMDKIPKAAIEPNTFEVTFRGINSVQKSNKKLKYGQMRIGGHMFRIPYTYC
FESLTLGDDVQYVLGCNFIRNMHGGVRLEGTEVTFYKNITTIQTRLMAPIVEEDDEDD
EVEQETNWIRTMASSTMDAPYEKLRSSFGPLMKELKDQGYIGENPMKHWARNKVLCYL
DIKNPDMVIEDKPIKHVTPQMEESFRKHIKGLLELKVIRPSTSKHRTTAFIVNSGTSV
DPVTGKETKGKERMVFNYKRLNDLTEKDQYSLPGINTIMKRVGHAKIYSKFDLKSGFH
QVAMHPESIKWTAFWVPDGLYEWLVMPFGLKNAPAVFQRKMDNVFKGTEAFIAVYIDD
ILVFSQSEEEHIKHIRVMLEKCRENGLVLSPTKMKIAQRKVEFLGAILEAGRIQLQPH
IIKKVVNFKDEDLQETKGLRSFLGLLNYARAYIPQMGRLLSPLYAKVSPKGEKKLNKE
DWKLVRQIKEQVQQLPDLELPPQECFIILETDGCMEGWGGVCKWKKKEYDPKNTERIC
AYASGKFHPIKSTIDAEIHAVMNSLEAFKIHYLDKPSLTIRTDCQAIIAFFDTISNHK
PSRLRWINFTDYVTGLGIPVNFQHVKGEDNRLADALSRLISVIFEAGQQWQEESTRMQ
HLTQLIGEHYKFEASGKKHHQLKETLTALITSTMNSWTEKSLSSTQKEALLSDTRITS
TNKWPLLKDKPVHKQNKHSNNWKEYKDKRRTTSGSQRQETTGPVTGYHPP"
misc_feature 3600..3650
/locus_tag="BU35_gp3"
/note="zinc finger; Region: ZnF_C2HC; smart00343"
/db_xref="CDD:197667"
misc_feature <4113..>4340
/locus_tag="BU35_gp3"
/note="Outer membrane protein (OmpH-like); Region: OmpH;
pfam03938"
/db_xref="CDD:252261"
misc_feature 4506..4763
/locus_tag="BU35_gp3"
/note="Cellular and retroviral pepsin-like aspartate
proteases; Region: pepsin_retropepsin_like; cl11403"
/db_xref="CDD:264354"
misc_feature order(4524..4526,4530..4532,4536..4538,4605..4613,
4740..4742)
/locus_tag="BU35_gp3"
/note="inhibitor binding site; inhibition site"
/db_xref="CDD:133137"
misc_feature 4524..4532
/locus_tag="BU35_gp3"
/note="catalytic motif [active]"
/db_xref="CDD:133137"
misc_feature 4524..4526
/locus_tag="BU35_gp3"
/note="Catalytic residue [active]"
/db_xref="CDD:133137"
misc_feature order(4605..4616,4623..4634)
/locus_tag="BU35_gp3"
/note="Active site flap [active]"
/db_xref="CDD:133137"
misc_feature 5157..5714
/locus_tag="BU35_gp3"
/note="RT_LTR: Reverse transcriptases (RTs) from
retrotransposons and retroviruses which have long terminal
repeats (LTRs) in their DNA copies but not in their RNA
template. RT catalyzes DNA replication from an RNA
template, and is responsible for the...; Region: RT_LTR;
cd01647"
/db_xref="CDD:238825"
misc_feature 5241..5717
/locus_tag="BU35_gp3"
/note="Reverse transcriptase (RNA-dependent DNA
polymerase); Region: RVT_1; pfam00078"
/db_xref="CDD:249567"
misc_feature order(5379..5396,5481..5486,5562..5564,5568..5573,
5703..5708)
/locus_tag="BU35_gp3"
/note="putative active site [active]"
/db_xref="CDD:238825"
misc_feature order(5379..5396,5481..5483,5568..5570)
/locus_tag="BU35_gp3"
/note="putative NTP binding site [chemical binding]; other
site"
/db_xref="CDD:238825"
misc_feature 5484..5486
/locus_tag="BU35_gp3"
/note="putative nucleic acid binding site [nucleotide
binding]; other site"
/db_xref="CDD:238825"
misc_feature 6003..6386
/locus_tag="BU35_gp3"
/note="Ribonuclease H-like superfamily, including RNase H,
HI, HII, HIII, and RNase-like domain IV of spliceosomal
protein Prp8; Region: RNase_H_like; cl14782"
/db_xref="CDD:265450"
misc_feature order(6015..6026,6132..6137,6144..6146,6222..6224,
6345..6347,6381..6383)
/locus_tag="BU35_gp3"
/note="RNA/DNA hybrid binding site [nucleotide binding];
other site"
/db_xref="CDD:259998"
misc_feature order(6015..6017,6222..6224)
/locus_tag="BU35_gp3"
/note="active site"
/db_xref="CDD:259998"
gene 6416..6847
/locus_tag="BU35_gp4"
/db_xref="GeneID:18388308"
CDS 6416..6847
/locus_tag="BU35_gp4"
/note="ORF4"
/codon_start=1
/product="hypothetical protein"
/protein_id="YP_009002586.1"
/db_xref="GI:586929339"
/db_xref="GeneID:18388308"
/translation="MAGRINKNAASDTANRRALQIRGIGEEAPSTERDTHRLDNFYHE
LMDREITLLHTEGSIAVRYKDHIHQQMAIIERQACAQTEQALKQLERIQRQKEDYIWQ
SATRDNWASDRLPSTIARNNALSSRIEGIKGLINDIKRTPL"
ORIGIN
1 tggtatcaga gcatggtttc cgatagtcat gtccggctaa aaacctttca aggtctgggg
61 aaatagagga gaagtgtgta gctgggaaat actgtttttg tactattctt cctgtcaacc
121 aaaataattg agtcttcctg tagtagttgg gctaaaaggg caaaaggcga aggcactata
181 tggtgttagg caaggaggcc gttaacctga accccggaga tgagtctttt tagttaacaa
241 accaggggaa gcaattatga gtaggagatg ggaggaagaa atacagaagt ggtatgaaca
301 gtcaagcact tcaagtctag agtacctaga tcttgcttca acatcaaaag tttcaaacca
361 acaattagct cacaatctct ctgttattta caaccatagt agtctttcat ccagagtcca
421 aatcaaaaat tacaaaaaga ttcaagagtc cctagatagt attgagtctc gaatctcaaa
481 gcttgaaagt aatctcaaaa acttaaccaa aaccttcatt gcaaatagac ctttaaccca
541 ggctgaggtc actaccttag tctctgaaat tagccagcag ccaaagttgg tagaagccca
601 agctctccaa ctcacagaag atctcaacaa aaagctaacg agggttgaaa aacttttgca
661 caagctagag cagtggtccc agtcttgatg agccacagcc gtaccagggc aactgcaagc
721 tacaaagaag cagtcgcagc cactgaagac atccagccac cagcagttgg atttgccaaa
781 ccatctgaca acgtaggagc tcaatcgtca agttctgtta tcatcaagca gaataacacg
841 attatacaac tgttagcttc tctttctgaa aaagttaaca cgcttgaaac agaggttaga
901 cacatcaaaa acgtgtttga ctctcgtgag ccctctacat caacgttacc aacagacctg
961 attgaaaagc tagaaggact gacgcttaca gacaaacagg aacggccaaa agagagaaag
1021 ggaacccttt ttgctttcaa ggacccttac gtgattctcc aggaagaaaa agagaaactg
1081 aaaaaggcga catgagtact acaactactc agacggtacc accagtcacg agaaacgcca
1141 ctagaaggca gcaaggagaa agagagactc ctctctttga ggaccagatt cgtgactacc
1201 gtaacagcca aagacgcgcc tatacaacac aaagagctgt gagaaggctc aggagaagaa
1261 ttaccggccg acaaccatat caggaaactc tggagcagga agttgaccca caagcagacc
1321 tccagatgtc catgcaagaa cgggcaagat tagtaccagc tgaggtatta tacagatcca
1381 ggagagacac gattcaccat cgtgtttaca gtcatagatc ggaggaagca atcctttgta
1441 cagatgggaa tcaggtggat agaactttta tccagcctga aagtatggaa gccctacaaa
1501 ggacaggcat atcctttatt catataggga ttatgcaagt tagactacag attcttcacc
1561 ggcagaatga aggaaccatg gctttagtcg tcttccggga tacaagatgg aatggagacc
1621 agtcaatctt tgcaaccatg gaggtagatc tgactgaggg aagacaaatc atctacgttg
1681 taccagacac catgatgacc attggtgatt ttgcaagaaa tatacagata tccatactaa
1741 cccgtggata ttctaactgg cagaatggtg aggcaaatct cttagtaaca agaggcatag
1801 ttgggagact atccaatact cctaacgttg cttttgccta tgagattggc aacgtaactg
1861 actacctgac aagccaagga gtacaagcca tagcaggaag gagatatgac agcagggagt
1921 tgcagaacca gcagtggatc ttaagacctc cacaggtgga aagattgcca caagcaccga
1981 aaaacgttga cacaagaaat ctcattgatg gttcaatatc tttgagattt agagattatg
2041 caacaacctc aacaccagag ctaccatcct acaacgaaca ggatgaagag gtggacccag
2101 atgaggagca gctgatggaa catgtagtag ctgcctttgt agaggaagaa cccaaggaaa
2161 tttgggacac actgggacaa ccttctggga agtttgactt cctggtaaaa tattcagcac
2221 cggcatcctc gaagatcagg attgaggata ttgttcccac cggatggggt gatgaatggg
2281 aacaaccgga acagcaacaa tcgccacagc aacaacaact actatcatgc tctcaggagg
2341 agagtgatga tgaggatgat tgggtttcca gatttcaatt tctggcccaa tttgatgaag
2401 aaccagccac tcaaccacag agcccacaaa gcccaacatg gtataaccca tttgcagaag
2461 gtggtgggag tgatgaaaat gatgaaaacc cagacccaga agaaagtgaa gaaatgggta
2521 atgaaatggg ttatgaaacg gaggtggagg aacaggaaga gaaggtagcc atgatacaag
2581 aagatccaga cttgctacca taccctcacc taagggaact gctgaaaaat gaagaaaagg
2641 tgttttcaac acagcaatcg gaagtgagca gttaccgacc acctcaagat acagcaatga
2701 ctccagcagg atatgcacca gcaagaggag aggcatcatc ctctacgcat caacccatgt
2761 ttgaagggta tatgccaaga agaccacaat tcaagcaaga cagatcaacg gagtactggc
2821 aattcccaac agcacaggga caaacaggag ccatgtttgt cattcccaga cagattggct
2881 tatttcatga tgcgttcaac agatgggagt ctataacaaa gaactatgtt gcttcccagg
2941 gatttactga ttctaaagac aaagcagaat tcatggaaaa tttgcttgga gaaacggaga
3001 agctaacatg ggttcaatgg cgaatgaact ttcctacaga ataccaggag atggtaaacg
3061 cagctgatgg tagggaaggg acgcagaaca tactctctca aatgaggaga gtattcactc
3121 ttgaagatcc tacaactgga tctactgcag tacaggacga ggcatacaga gacttggaga
3181 ggctaagctg caccaacatc aaagacatag tctccttttt aaatgactat gcaagacttg
3241 cagcaaaatc gggaaggatg ttcatcagcc cagaattatc agaaaagctc tggttgaaga
3301 tgccttatga tttggggaac aaaatacgta ctgcatacga ggcaaaacat gagggaaaca
3361 ctattggtgt tttcccaagg atcctattca catacaagta cctggagcaa gaatgcaagg
3421 aggcagcatt caggaggtct ctgaaaaatt tatctttctg ttcagagatg ccattgccag
3481 gatactatgg agaaaagaag cgctacggtg taagaaggtc aacaacatac aaaggaaagc
3541 cacacccaac acatgcaaga attgagaagc agaaacacct tgtcagaaat aaaaggtgta
3601 aatgttattt gtgtggagag atggggcact ttgccagaga atgccctaat gacaagaaga
3661 atacacgccg agtagccatg tacgagcaac tgaacatacc agaaggattt gaggtagtgt
3721 cagttgaaga aggagaagag gtcagtgatg ctatcttcag catctctgaa aatgaagatg
3781 aagatgccct gcacacaggc ggagcctttg ctgaaactat ctttatgttt catgaaggca
3841 cagacaatgt ttattgggca ggaaagaagg atggttacct cccatacaag aggataacca
3901 gggaacaatt ccagtgtgag catgaatggc agatgaacca ggaagtagcc actgtggaac
3961 tggaaaaatg ttccttttgc aagcaagaaa ccaggaggag gatgagactg cattgcccta
4021 aatgcagaat tacaagttgt gtcctctgca gcagtaatta ctgtggtatt gcaatcaaac
4081 cagaacctgt acagaccagc ttcttcaatg agaagagtct gttaacccaa cagcaagaat
4141 acattgcata ccagactgct gaaatcaaga agcttcagga gcagttaaaa caaacagaag
4201 aaaaactggc agaagctgaa agaaaaatca ggggatacga gaacatggaa gtcctagaga
4261 aagaaagaaa ggaaatggcg ctgaaagata tgcagagaaa aattgatgaa gaaataaaga
4321 aagcagccat gcaggaactt ccaactgaaa aggagctgtg ggaacaacga gaaggaaaag
4381 cccacattgc aggagctgta atagcagcat caacagaaac tggaagagaa gaaagaaggc
4441 cagtcaaagg gaacatgcta tacaacatgg acgtagaaat agacataccg tctgttccaa
4501 ggttcactgt taaagcaatc ctggacaccg gagcaacgtc gtgctgcatc agtatggata
4561 agataccaaa agcagccatc gagcctaata cttttgaagt aacattcaga ggtattaatt
4621 ctgtgcagaa gagtaacaag aagctgaagt atggccagat gagaattgga ggacatatgt
4681 tcagaatacc ctacacctac tgctttgaat cactgacatt gggagatgat gtgcagtatg
4741 ttttgggttg caattttatc agaaatatgc acggaggagt caggcttgaa ggaacagagg
4801 tgacgttcta caaaaatatc accaccattc agacaagatt aatggcgcca atcgttgaag
4861 aagatgatga agacgatgaa gtggagcagg agactaactg gattagaacc atggcatcat
4921 caacaatgga cgctccatat gagaagctga ggagcagctt tggcccatta atgaaggagt
4981 taaaagacca ggggtacatt ggtgaaaatc caatgaaaca ttgggctcgg aacaaagtac
5041 tgtgctatct ggacatcaaa aacccagata tggtgataga ggataagccc attaaacacg
5101 tgacgcccca gatggaggaa tctttccgga aacatataaa gggcctgttg gaactgaagg
5161 tgattaggcc cagcactagt aaacatcgga ccacagcctt cattgtcaac tcaggcacaa
5221 gcgtggatcc agtaaccgga aaagagacca aaggaaagga gagaatggtg ttcaactaca
5281 aaaggctgaa tgatctcact gagaaagatc agtacagtct gccaggcatc aacaccatta
5341 tgaagagggt tggccatgct aagatctact caaagtttga tctcaagagt ggatttcatc
5401 aggtggctat gcacccggag tctatcaagt ggactgcttt ctgggttcca gatggactgt
5461 atgaatggct agttatgcca tttggactta aaaacgcccc agcagtattt cagcgaaaga
5521 tggacaacgt cttcaaaggt actgaagcct ttatcgctgt atacattgat gacatactcg
5581 tattttccca gtctgaggag gaacatatca agcacatacg ggtcatgtta gaaaaatgcc
5641 gggagaatgg gcttgttctt agcccaacaa aaatgaagat agcccagagg aaggtggaat
5701 ttctgggagc tattctggaa gcaggccgta ttcagctgca gccccatatc ataaagaagg
5761 tcgtgaactt caaagacgaa gacctgcagg aaacgaaggg cctcagatcc tttttgggcc
5821 tacttaatta tgccagagca tatatcccac aaatgggtcg tctgctaagc ccattatatg
5881 ccaaggtgag ccctaaagga gagaagaaac tgaacaaaga agactggaag ctggtcagac
5941 agatcaaaga acaagttcag cagctaccag atcttgaatt accccctcaa gaatgtttta
6001 tcattcttga aactgacgga tgtatggaag gatggggagg agtttgcaaa tggaagaaga
6061 aggagtatga tcctaaaaat actgaaagga tttgtgccta tgccagtggg aagtttcatc
6121 caatcaaatc aaccatagat gcagaaatcc atgctgtgat gaactccctt gaagccttca
6181 aaatccatta cctggacaaa ccatccttaa ccataagaac agactgccag gccatcatag
6241 ccttctttga cactatcagt aatcataaac catctagact taggtggata aattttactg
6301 attatgttac aggattaggc attccagtta atttccagca tgttaaaggt gaagataata
6361 gattagctga tgctttatca aggttaatct ctgttatctt tgaagcagga caacaatggc
6421 aggaagaatc aacaagaatg cagcatctga cacagctaat aggagagcac tacaaattcg
6481 aggcatcggg gaagaagcac catcaactga aagagacact caccgccttg ataacttcta
6541 ccatgaactc atggacagag aaatcactct cctccacaca gaaggaagca ttgctgtcag
6601 atacaaggat cacatccacc aacaaatggc cattattgaa agacaagcct gtgcacaaac
6661 agaacaagca ctcaaacaac tggaaagaat acaaagacaa aaggaggact acatctggca
6721 gtcagcgaca agagacaact gggccagtga caggttacca tccaccatag ccagaaacaa
6781 cgcactcagc agcaggatag agggaatcaa aggactgata aatgacataa agaggacccc
6841 tctgtgataa tggagcgtga tggaccctgg accagaataa agaggttaat tccactcaga
6901 ccgaaattgt ggaaataatt atgtatgctt taataattga gtcaggtaag aaaggaaatc
6961 tgttgtcctt atctacctaa agtcagtatc atgggtgaga aagggttttg tctttgtccc
7021 ccactaaata aaagtcgagt cacgggcagt aaaggcattt gtccttaccc gtctagtgga
7081 gtactaaatt atgaggatgg aaattattat gctgacgatg gggcccaatg agcacccggg
7141 catcactttt ccttccttat aaatagctta gttatctcat agctaagatc acggaagaaa
7201 tacttcagat cttactttga gctttgtaag tgttcttgag tctagtgttt gtaatctttc
7261 ttgttcttta ataaattcca gtccctcgga ctttaaacca ttttcattga tcctgtttac
7321 tctgtttata ctgttgctat cgtgaattgt atgttgccag aacctcactg taaggttatt
7381 ggatcctcat agttttcacg ccggcaaccg tattccgccg cttccgcccc ctctt
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