TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= TBG_YEAST P53378 Tubulin gamma chain (Gamma-tubulin) Length=473 Score E Sequences producing significant alignments: (Bits) Value scaffold-423 197 2e-57 scaffold-57 156 9e-41 unplaced-5 155 1e-40 unplaced-665 144 9e-37 scaffold-26 132 2e-33 scaffold-157 29.6 4.0 > scaffold-423 Length=1446679 Score = 197 bits (502), Expect(3) = 2e-57, Method: Compositional matrix adjust. Identities = 140/396 (35%), Positives = 234/396 (59%), Gaps = 37/396 (9%) Frame = -2 Query 88 DPRNTWVASDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvaggtg 147 +P N + + DG+ AGN+WA+GY G R +D+ + +D+E D+ ++ EGF L+HS+AGGTG Sbjct 1101321 NPENVY*S*DGSGAGNNWASGYQSGGRLSEDLFDILDREADNAESLEGFNLMHSIAGGTG 1101142 Query 148 sglgsnllEALCDRYPKKILTTYSVFPARS---SEVVVQSYNTILALRRLIEDSDATVVF 204 SGLGS LLE L DR+PKK++ TYSVFP+ + S+VVVQ YN++L+L+RL E++D V Sbjct 1101141 SGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSVLSLKRLTENADMVTVL 1100962 Query 205 DNASLLNISGKVFRNPNIDLQHTNQListiissvtnsiRFPsymyssmssiysTLIPSPE 264 DNA+L ++ N N+++STI+++ + +RFPSY Y+SM + S+L+P + Sbjct 1100961 DNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMSSLVPIRK 1100782 Query 265 LHFLSPSFTPFTSDYIHD------------------------DIAHKGHSSYDVMLDLLD 300 H+++ +++PFT + + D + K S D+M LL Sbjct 1100781 CHYITCAYSPFTHNDLRDYAASSNVNAAASENSANAVNRSR*SLMKKAGLS-DIMRRLL* 1100605 Query 301 PSNSLVSTAMNNP--TYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNI 356 + ++S N P + + + G+V + + +A+ KL+++ ++F W S+M +N+ Sbjct 1100604 TKSRVISVE-NTPLNCFLSAMTLV*GDVGAKDVHKAILKLKEKNTMQFAPWKPSSM*INV 1100428 Query 357 GRRSPYLP-LQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQS 415 RRSP L P VSG+ML+N ++V +F++ + +D++ + AFL Y D+F Sbjct 1100427 ARRSPCLEDGDPQSGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKLDMF-- 1100254 Query 416 MQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLV 451 ++ +EF SRE + + E+Y+A EQ YL + V Sbjct 1100253 -KDGLEEFDVSRESLLEVTEEYLACEQADYLSKMKV 1100149 Score = 38.1 bits (87), Expect(3) = 2e-57, Method: Compositional matrix adjust. Identities = 22/65 (34%), Positives = 29/65 (45%), Gaps = 6/65 (9%) Frame = -1 Query 17 VGKFLWSQLAKEHAIGTDGLSQLP------DSSTERDDDTKPFFRENSRNKFTPRAIMMD 70 VG W+QL EH + DGL SS D FF + + PR+I++D Sbjct 1101622 VGMEFWAQLCAEHGLAPDGLPLTSSD****SSSPPLMDRKDIFFYQAEDGHYIPRSILVD 1101443 Query 71 SEPSV 75 EP V Sbjct 1101442 LEPRV 1101428 Score = 26.9 bits (58), Expect(3) = 2e-57, Method: Compositional matrix adjust. Identities = 11/15 (73%), Positives = 12/15 (80%), Gaps = 0/15 (0%) Frame = -3 Query 1 MGGEIITLQAGQCGN 15 M EII+LQ GQCGN Sbjct 1101734 MSREIISLQVGQCGN 1101690 Score = 199 bits (507), Expect(3) = 1e-56, Method: Compositional matrix adjust. Identities = 142/396 (36%), Positives = 235/396 (59%), Gaps = 34/396 (9%) Frame = +3 Query 86 FFDPRNTWVASDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvagg 145 +P N + + DG+ AGN+WA+GY G R +D+ + +D+E D++++ EGF L+HS+AGG Sbjct 190323 LHNPENVY*S*DGSGAGNNWASGY*SGERLSEDLFDILDREADNSESLEGFNLMHSIAGG 190502 Query 146 tgsglgsnllEALCDRYPKKILTTYSVFPARS---SEVVVQSYNTILALRRLIEDSDATV 202 TGSGLGS LLE L DR+PKK++ TYSVFP+ + S+VVVQ YN++L+L+RL E++D Sbjct 190503 TGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSLLSLKRLTENADMVT 190682 Query 203 VFDNASLLNISGKVFRNPNIDLQHTNQListiissvtnsiRFPsymyssmssiysTLIPS 262 V DNA+L ++ N N+++STI+++ + +RFPSY Y+SM + S+L+P Sbjct 190683 VLDNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMSSLVPI 190862 Query 263 PELHFLSPSFTPFTSDYIHDDIA----------------------HKGHSSYDVMLDLLD 300 + H+++ ++TPFT + + D A K D+M LL Sbjct 190863 RKCHYITCAYTPFTHNDLRDYAAISNVNAASENSTNAVNRSR*SLMKKAGLSDIMRRLL* 191042 Query 301 PSNSLVSTAMNNP--TYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNI 356 P + ++S N P Y + + + G+V + + +A+ KL+++ ++F W S+M +N+ Sbjct 191043 PKSRVISVE-NTPLNCYLSAMSIVQGDVGAKDVHKAILKLKEKNTMQFAPWKPSSM*INV 191219 Query 357 GRRSPYLPLQPN-ENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQS 415 RRSP L VSG+ML+N ++V +F++ + +D++ + AFL Y D+F Sbjct 191220 ARRSPCLEDGDT*GGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKLDMF-- 191393 Query 416 MQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLV 451 ++ +EF SRE + + E+Y+A EQ YL + V Sbjct 191394 -KDGLEEFDVSRESLLDVTEEYLACEQADYLSKMKV 191498 Score = 33.1 bits (74), Expect(3) = 1e-56, Method: Compositional matrix adjust. Identities = 21/75 (28%), Positives = 28/75 (37%), Gaps = 16/75 (21%) Frame = +2 Query 17 VGKFLWSQLAKEHAIGTDGLSQLP----------------DSSTERDDDTKPFFRENSRN 60 VG W+QL EH + DGL S D FF + Sbjct 189980 VGMEFWAQLCAEHGLAPDGLPLTSSD**************SPSIPLMDRKDIFFYQAEDG 190159 Query 61 KFTPRAIMMDSEPSV 75 + PR+I++D EP V Sbjct 190160 HYIPRSILIDLEPRV 190204 Score = 26.6 bits (57), Expect(3) = 1e-56, Method: Compositional matrix adjust. Identities = 11/15 (73%), Positives = 12/15 (80%), Gaps = 0/15 (0%) Frame = +3 Query 1 MGGEIITLQAGQCGN 15 M EII+LQ GQCGN Sbjct 189867 MSREIISLQVGQCGN 189911 > scaffold-57 Length=110052 Score = 156 bits (394), Expect = 9e-41, Method: Compositional matrix adjust. Identities = 132/448 (29%), Positives = 215/448 (48%), Gaps = 18/448 (4%) Frame = -2 Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFT 63 E I++ GQ G +G W EH I DG S DD FF E K Sbjct 88580 ECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGKHV 88401 Query 64 PRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILN 121 PR + D EP+V+ +V T+R F P + A N++A G Y +G D +L Sbjct 88400 PRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTVGKEIIDSVLE 88227 Query 122 KIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCDRYPKKILTTYSVFPA-RSSEV 180 ++ K D+ GF + HS GGTGSG G+ LLE L Y KK ++V+PA + S Sbjct 88226 RVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQVSTA 88047 Query 181 VVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQListiissvtn 240 VV+ YN+IL +E SD + DN ++ +I + + N+LI+ ++SS+T Sbjct 88046 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 87867 Query 241 siRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLD 300 S+RF + ++ + L+P P +HF ++ P S + H+ S +++ + Sbjct 87866 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISA---EKAYHEQLSVHEITQACFE 87696 Query 301 PSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMT--KLQQRIKFPSWSSSAMHVNIGR 358 P+N +V N Y G+V P+ ++ A+ K ++ I+F W + V I Sbjct 87695 PNNQMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 87516 Query 359 RSPYLPLQPNENEV--SGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSM 416 + P + + +V + MLSN + + + + FD ++AK AF++ Y VG+ + Sbjct 87515 QPPTVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWY-VGEGME-- 87345 Query 417 QNVQDEFAESREVVQSLMEDYVAAEQDS 444 + EF+E+RE + +L +DY DS Sbjct 87344 ---EGEFSEAREDLAALEKDYEEVGTDS 87270 > unplaced-5 Length=12763 Score = 155 bits (393), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 132/448 (29%), Positives = 215/448 (48%), Gaps = 18/448 (4%) Frame = +2 Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFT 63 E I++ GQ G +G W EH I DG S DD FF E K Sbjct 3263 ECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGKHV 3442 Query 64 PRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILN 121 PR + D EP+V+ +V T+R F P + A N++A G Y +G D +L Sbjct 3443 PRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTVGKEIIDSVLE 3616 Query 122 KIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCDRYPKKILTTYSVFPA-RSSEV 180 ++ K D+ GF + HS GGTGSG G+ LLE L Y KK ++V+PA + S Sbjct 3617 RVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQISTA 3796 Query 181 VVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQListiissvtn 240 VV+ YN+IL +E SD + DN ++ +I + + N+LI+ ++SS+T Sbjct 3797 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 3976 Query 241 siRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLD 300 S+RF + ++ + L+P P +HF ++ P S + H+ S +++ + Sbjct 3977 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISA---EKAYHEQLSVHEITQACFE 4147 Query 301 PSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMT--KLQQRIKFPSWSSSAMHVNIGR 358 P+N +V N Y G+V P+ ++ A+ K ++ I+F W + V I Sbjct 4148 PNNQMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 4327 Query 359 RSPYLPLQPNENEV--SGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSM 416 + P + + +V + MLSN + + + + FD ++AK AF++ Y VG+ + Sbjct 4328 QPPTVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWY-VGEGME-- 4498 Query 417 QNVQDEFAESREVVQSLMEDYVAAEQDS 444 + EF+E+RE + +L +DY DS Sbjct 4499 ---EGEFSEAREDLAALEKDYEEVGTDS 4573 > unplaced-665 Length=20473 Score = 144 bits (363), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 125/467 (27%), Positives = 213/467 (46%), Gaps = 53/467 (11%) Frame = -2 Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPF---------- 53 EI+ L G CGN +G W ++ EH I +G + DS + + F Sbjct 7230 EIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYE-GDSDLQLERINGEFLNVLFCA**A 7054 Query 54 -----------FRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASA 101 F E S K+ PRA+++D EP + V + F P N GA Sbjct 7053 FCITLLLIVVYFNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGA-- 6880 Query 102 GNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCD 160 GN+WA G Y G D +L+ + KE + D +GFQ+ HS+ GGTG+G+G+ L+ + + Sbjct 6879 GNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIRE 6700 Query 161 RYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRN 219 YP +++ T+SV P+ + S+ VV+ YN L++ +L+E+SD T DN +L +I + + Sbjct 6699 EYPDRMMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKL 6520 Query 220 PNIDLQHTNQListiissvtnsiRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDY 279 N L+S ++S VT SIRFP + + + + ++P P LHF F P TS Sbjct 6519 TTPTYGDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTS-- 6346 Query 280 IHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQ 339 ++ S ++ + D N + ++ + Y V G + +++ M +Q Sbjct 6345 -RGSQQYRALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQ 6169 Query 340 QR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKV 397 + F W + + ++ + P ++S + N + + +F+ + F + Sbjct 6168 TKNSSYFVEWIPNNV------KTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVM 6007 Query 398 FAKGAFLNNY---------------NVGDLFQSMQNVQDEFAESREV 429 F + AFL+ Y N+ DL Q QD AE ++ Sbjct 6006 FKRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEDM 5866 > scaffold-26 Length=483268 Score = 132 bits (331), Expect(2) = 2e-33, Method: Compositional matrix adjust. Identities = 108/397 (27%), Positives = 190/397 (48%), Gaps = 31/397 (8%) Frame = -3 Query 53 FFRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YD 110 ++ E S K+ PRA+++D EP + V + F P N GA GN+WA G Y Sbjct 109790 YYNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGA--GNNWAKGHYT 109617 Query 111 IGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCDRYPKKILTTY 170 G D +L+ + KE + D +GFQ+ HS+ GGTG+G+G+ L+ + + YP +++ T+ Sbjct 109616 EGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDRMMCTF 109437 Query 171 SVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQ 229 SV P+ + S+ VV+ YN L++ +L+E+SD T DN +L +I + + N Sbjct 109436 SVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLTTPTYGDLNH 109257 Query 230 ListiissvtnsiRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGH 289 L+S ++S VT SIRFP + + + + ++P P LHF F P TS ++ Sbjct 109256 LVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTS---RGSQQYRAL 109086 Query 290 SSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSW 347 S ++ + D N + ++ + Y V G + +++ M +Q + F W Sbjct 109085 SVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNSSYFVEW 108906 Query 348 SSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNY 407 + + ++ + P ++S + N + + +F+ + F +F + AFL+ Y Sbjct 108905 IPNNV------KTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWY 108744 Query 408 ---------------NVGDLFQSMQNVQDEFAESREV 429 N+ DL Q QD AE ++ Sbjct 108743 TGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEDM 108633 Score = 28.9 bits (63), Expect(2) = 2e-33, Method: Compositional matrix adjust. Identities = 13/32 (41%), Positives = 18/32 (56%), Gaps = 0/32 (0%) Frame = -2 Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDG 35 EI+ L G CGN +G W ++ EH I +G Sbjct 110001 EIVHL*TG*CGN*IGAKFWEVISDEHGIDPNG 109906 > scaffold-157 Length=706690 Score = 29.6 bits (65), Expect = 4.0, Method: Compositional matrix adjust. Identities = 13/38 (34%), Positives = 22/38 (58%), Gaps = 0/38 (0%) Frame = +3 Query 96 SDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNF 133 ++GA+ S A+GY G +Q +LN +D + S +N Sbjct 263277 AEGATLIGSTADGYSYGVYSQRQVLNLVDMYLRSMNNL 263390 Lambda K H a alpha 0.317 0.133 0.387 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2892629495 Database: X5.fasta Posted date: Nov 10, 2021 1:52 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40