TBLASTN 2.8.1+
Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.
Database: X5.fasta
1,868 sequences; 23,962,143 total letters
Query= TBG_YEAST P53378 Tubulin gamma chain (Gamma-tubulin)
Length=473
Score E
Sequences producing significant alignments: (Bits) Value
scaffold-423 197 2e-57
scaffold-57 156 9e-41
unplaced-5 155 1e-40
unplaced-665 144 9e-37
scaffold-26 132 2e-33
scaffold-157 29.6 4.0
> scaffold-423
Length=1446679
Score = 197 bits (502), Expect(3) = 2e-57, Method: Compositional matrix adjust.
Identities = 140/396 (35%), Positives = 234/396 (59%), Gaps = 37/396 (9%)
Frame = -2
Query 88 DPRNTWVASDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvaggtg 147
+P N + + DG+ AGN+WA+GY G R +D+ + +D+E D+ ++ EGF L+HS+AGGTG
Sbjct 1101321 NPENVY*S*DGSGAGNNWASGYQSGGRLSEDLFDILDREADNAESLEGFNLMHSIAGGTG 1101142
Query 148 sglgsnllEALCDRYPKKILTTYSVFPARS---SEVVVQSYNTILALRRLIEDSDATVVF 204
SGLGS LLE L DR+PKK++ TYSVFP+ + S+VVVQ YN++L+L+RL E++D V
Sbjct 1101141 SGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSVLSLKRLTENADMVTVL 1100962
Query 205 DNASLLNISGKVFRNPNIDLQHTNQListiissvtnsiRFPsymyssmssiysTLIPSPE 264
DNA+L ++ N N+++STI+++ + +RFPSY Y+SM + S+L+P +
Sbjct 1100961 DNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMSSLVPIRK 1100782
Query 265 LHFLSPSFTPFTSDYIHD------------------------DIAHKGHSSYDVMLDLLD 300
H+++ +++PFT + + D + K S D+M LL
Sbjct 1100781 CHYITCAYSPFTHNDLRDYAASSNVNAAASENSANAVNRSR*SLMKKAGLS-DIMRRLL* 1100605
Query 301 PSNSLVSTAMNNP--TYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNI 356
+ ++S N P + + + G+V + + +A+ KL+++ ++F W S+M +N+
Sbjct 1100604 TKSRVISVE-NTPLNCFLSAMTLV*GDVGAKDVHKAILKLKEKNTMQFAPWKPSSM*INV 1100428
Query 357 GRRSPYLP-LQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQS 415
RRSP L P VSG+ML+N ++V +F++ + +D++ + AFL Y D+F
Sbjct 1100427 ARRSPCLEDGDPQSGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKLDMF-- 1100254
Query 416 MQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLV 451
++ +EF SRE + + E+Y+A EQ YL + V
Sbjct 1100253 -KDGLEEFDVSRESLLEVTEEYLACEQADYLSKMKV 1100149
Score = 38.1 bits (87), Expect(3) = 2e-57, Method: Compositional matrix adjust.
Identities = 22/65 (34%), Positives = 29/65 (45%), Gaps = 6/65 (9%)
Frame = -1
Query 17 VGKFLWSQLAKEHAIGTDGLSQLP------DSSTERDDDTKPFFRENSRNKFTPRAIMMD 70
VG W+QL EH + DGL SS D FF + + PR+I++D
Sbjct 1101622 VGMEFWAQLCAEHGLAPDGLPLTSSD****SSSPPLMDRKDIFFYQAEDGHYIPRSILVD 1101443
Query 71 SEPSV 75
EP V
Sbjct 1101442 LEPRV 1101428
Score = 26.9 bits (58), Expect(3) = 2e-57, Method: Compositional matrix adjust.
Identities = 11/15 (73%), Positives = 12/15 (80%), Gaps = 0/15 (0%)
Frame = -3
Query 1 MGGEIITLQAGQCGN 15
M EII+LQ GQCGN
Sbjct 1101734 MSREIISLQVGQCGN 1101690
Score = 199 bits (507), Expect(3) = 1e-56, Method: Compositional matrix adjust.
Identities = 142/396 (36%), Positives = 235/396 (59%), Gaps = 34/396 (9%)
Frame = +3
Query 86 FFDPRNTWVASDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvagg 145
+P N + + DG+ AGN+WA+GY G R +D+ + +D+E D++++ EGF L+HS+AGG
Sbjct 190323 LHNPENVY*S*DGSGAGNNWASGY*SGERLSEDLFDILDREADNSESLEGFNLMHSIAGG 190502
Query 146 tgsglgsnllEALCDRYPKKILTTYSVFPARS---SEVVVQSYNTILALRRLIEDSDATV 202
TGSGLGS LLE L DR+PKK++ TYSVFP+ + S+VVVQ YN++L+L+RL E++D
Sbjct 190503 TGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSLLSLKRLTENADMVT 190682
Query 203 VFDNASLLNISGKVFRNPNIDLQHTNQListiissvtnsiRFPsymyssmssiysTLIPS 262
V DNA+L ++ N N+++STI+++ + +RFPSY Y+SM + S+L+P
Sbjct 190683 VLDNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMSSLVPI 190862
Query 263 PELHFLSPSFTPFTSDYIHDDIA----------------------HKGHSSYDVMLDLLD 300
+ H+++ ++TPFT + + D A K D+M LL
Sbjct 190863 RKCHYITCAYTPFTHNDLRDYAAISNVNAASENSTNAVNRSR*SLMKKAGLSDIMRRLL* 191042
Query 301 PSNSLVSTAMNNP--TYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSWSSSAMHVNI 356
P + ++S N P Y + + + G+V + + +A+ KL+++ ++F W S+M +N+
Sbjct 191043 PKSRVISVE-NTPLNCYLSAMSIVQGDVGAKDVHKAILKLKEKNTMQFAPWKPSSM*INV 191219
Query 357 GRRSPYLPLQPN-ENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQS 415
RRSP L VSG+ML+N ++V +F++ + +D++ + AFL Y D+F
Sbjct 191220 ARRSPCLEDGDT*GGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKLDMF-- 191393
Query 416 MQNVQDEFAESREVVQSLMEDYVAAEQDSYLDDVLV 451
++ +EF SRE + + E+Y+A EQ YL + V
Sbjct 191394 -KDGLEEFDVSRESLLDVTEEYLACEQADYLSKMKV 191498
Score = 33.1 bits (74), Expect(3) = 1e-56, Method: Compositional matrix adjust.
Identities = 21/75 (28%), Positives = 28/75 (37%), Gaps = 16/75 (21%)
Frame = +2
Query 17 VGKFLWSQLAKEHAIGTDGLSQLP----------------DSSTERDDDTKPFFRENSRN 60
VG W+QL EH + DGL S D FF +
Sbjct 189980 VGMEFWAQLCAEHGLAPDGLPLTSSD**************SPSIPLMDRKDIFFYQAEDG 190159
Query 61 KFTPRAIMMDSEPSV 75
+ PR+I++D EP V
Sbjct 190160 HYIPRSILIDLEPRV 190204
Score = 26.6 bits (57), Expect(3) = 1e-56, Method: Compositional matrix adjust.
Identities = 11/15 (73%), Positives = 12/15 (80%), Gaps = 0/15 (0%)
Frame = +3
Query 1 MGGEIITLQAGQCGN 15
M EII+LQ GQCGN
Sbjct 189867 MSREIISLQVGQCGN 189911
> scaffold-57
Length=110052
Score = 156 bits (394), Expect = 9e-41, Method: Compositional matrix adjust.
Identities = 132/448 (29%), Positives = 215/448 (48%), Gaps = 18/448 (4%)
Frame = -2
Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFT 63
E I++ GQ G +G W EH I DG S DD FF E K
Sbjct 88580 ECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGKHV 88401
Query 64 PRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILN 121
PR + D EP+V+ +V T+R F P + A N++A G Y +G D +L
Sbjct 88400 PRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTVGKEIIDSVLE 88227
Query 122 KIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCDRYPKKILTTYSVFPA-RSSEV 180
++ K D+ GF + HS GGTGSG G+ LLE L Y KK ++V+PA + S
Sbjct 88226 RVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQVSTA 88047
Query 181 VVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQListiissvtn 240
VV+ YN+IL +E SD + DN ++ +I + + N+LI+ ++SS+T
Sbjct 88046 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 87867
Query 241 siRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLD 300
S+RF + ++ + L+P P +HF ++ P S + H+ S +++ +
Sbjct 87866 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISA---EKAYHEQLSVHEITQACFE 87696
Query 301 PSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMT--KLQQRIKFPSWSSSAMHVNIGR 358
P+N +V N Y G+V P+ ++ A+ K ++ I+F W + V I
Sbjct 87695 PNNQMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 87516
Query 359 RSPYLPLQPNENEV--SGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSM 416
+ P + + +V + MLSN + + + + FD ++AK AF++ Y VG+ +
Sbjct 87515 QPPTVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWY-VGEGME-- 87345
Query 417 QNVQDEFAESREVVQSLMEDYVAAEQDS 444
+ EF+E+RE + +L +DY DS
Sbjct 87344 ---EGEFSEAREDLAALEKDYEEVGTDS 87270
> unplaced-5
Length=12763
Score = 155 bits (393), Expect = 1e-40, Method: Compositional matrix adjust.
Identities = 132/448 (29%), Positives = 215/448 (48%), Gaps = 18/448 (4%)
Frame = +2
Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPFFRENSRNKFT 63
E I++ GQ G +G W EH I DG S DD FF E K
Sbjct 3263 ECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGKHV 3442
Query 64 PRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YDIGTRNQDDILN 121
PR + D EP+V+ +V T+R F P + A N++A G Y +G D +L
Sbjct 3443 PRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTVGKEIIDSVLE 3616
Query 122 KIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCDRYPKKILTTYSVFPA-RSSEV 180
++ K D+ GF + HS GGTGSG G+ LLE L Y KK ++V+PA + S
Sbjct 3617 RVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQISTA 3796
Query 181 VVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQListiissvtn 240
VV+ YN+IL +E SD + DN ++ +I + + N+LI+ ++SS+T
Sbjct 3797 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 3976
Query 241 siRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGHSSYDVMLDLLD 300
S+RF + ++ + L+P P +HF ++ P S + H+ S +++ +
Sbjct 3977 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISA---EKAYHEQLSVHEITQACFE 4147
Query 301 PSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMT--KLQQRIKFPSWSSSAMHVNIGR 358
P+N +V N Y G+V P+ ++ A+ K ++ I+F W + V I
Sbjct 4148 PNNQMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINY 4327
Query 359 RSPYLPLQPNENEV--SGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNYNVGDLFQSM 416
+ P + + +V + MLSN + + + + FD ++AK AF++ Y VG+ +
Sbjct 4328 QPPTVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWY-VGEGME-- 4498
Query 417 QNVQDEFAESREVVQSLMEDYVAAEQDS 444
+ EF+E+RE + +L +DY DS
Sbjct 4499 ---EGEFSEAREDLAALEKDYEEVGTDS 4573
> unplaced-665
Length=20473
Score = 144 bits (363), Expect = 9e-37, Method: Compositional matrix adjust.
Identities = 125/467 (27%), Positives = 213/467 (46%), Gaps = 53/467 (11%)
Frame = -2
Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDGLSQLPDSSTERDDDTKPF---------- 53
EI+ L G CGN +G W ++ EH I +G + DS + + F
Sbjct 7230 EIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYE-GDSDLQLERINGEFLNVLFCA**A 7054
Query 54 -----------FRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASA 101
F E S K+ PRA+++D EP + V + F P N GA
Sbjct 7053 FCITLLLIVVYFNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGA-- 6880
Query 102 GNSWANG-YDIGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCD 160
GN+WA G Y G D +L+ + KE + D +GFQ+ HS+ GGTG+G+G+ L+ + +
Sbjct 6879 GNNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIRE 6700
Query 161 RYPKKILTTYSVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRN 219
YP +++ T+SV P+ + S+ VV+ YN L++ +L+E+SD T DN +L +I + +
Sbjct 6699 EYPDRMMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKL 6520
Query 220 PNIDLQHTNQListiissvtnsiRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDY 279
N L+S ++S VT SIRFP + + + + ++P P LHF F P TS
Sbjct 6519 TTPTYGDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTS-- 6346
Query 280 IHDDIAHKGHSSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQ 339
++ S ++ + D N + ++ + Y V G + +++ M +Q
Sbjct 6345 -RGSQQYRALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQ 6169
Query 340 QR--IKFPSWSSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKV 397
+ F W + + ++ + P ++S + N + + +F+ + F +
Sbjct 6168 TKNSSYFVEWIPNNV------KTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVM 6007
Query 398 FAKGAFLNNY---------------NVGDLFQSMQNVQDEFAESREV 429
F + AFL+ Y N+ DL Q QD AE ++
Sbjct 6006 FKRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEDM 5866
> scaffold-26
Length=483268
Score = 132 bits (331), Expect(2) = 2e-33, Method: Compositional matrix adjust.
Identities = 108/397 (27%), Positives = 190/397 (48%), Gaps = 31/397 (8%)
Frame = -3
Query 53 FFRENSRNKFTPRAIMMDSEPSVIADVE-NTFRGFFDPRNTWVASDGASAGNSWANG-YD 110
++ E S K+ PRA+++D EP + V + F P N GA GN+WA G Y
Sbjct 109790 YYNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGA--GNNWAKGHYT 109617
Query 111 IGTRNQDDILNKIDKEIDSTDNFEGFQLLHsvaggtgsglgsnllEALCDRYPKKILTTY 170
G D +L+ + KE + D +GFQ+ HS+ GGTG+G+G+ L+ + + YP +++ T+
Sbjct 109616 EGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDRMMCTF 109437
Query 171 SVFPA-RSSEVVVQSYNTILALRRLIEDSDATVVFDNASLLNISGKVFRNPNIDLQHTNQ 229
SV P+ + S+ VV+ YN L++ +L+E+SD T DN +L +I + + N
Sbjct 109436 SVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLTTPTYGDLNH 109257
Query 230 ListiissvtnsiRFPsymyssmssiysTLIPSPELHFLSPSFTPFTSDYIHDDIAHKGH 289
L+S ++S VT SIRFP + + + + ++P P LHF F P TS ++
Sbjct 109256 LVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTS---RGSQQYRAL 109086
Query 290 SSYDVMLDLLDPSNSLVSTAMNNPTYFNVYNTIIGNVEPRQISRAMTKLQQR--IKFPSW 347
S ++ + D N + ++ + Y V G + +++ M +Q + F W
Sbjct 109085 SVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNSSYFVEW 108906
Query 348 SSSAMHVNIGRRSPYLPLQPNENEVSGMMLSNMSTVVNVFENACNTFDKVFAKGAFLNNY 407
+ + ++ + P ++S + N + + +F+ + F +F + AFL+ Y
Sbjct 108905 IPNNV------KTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWY 108744
Query 408 ---------------NVGDLFQSMQNVQDEFAESREV 429
N+ DL Q QD AE ++
Sbjct 108743 TGEGMDEMEFTEAESNMNDLVSEYQQYQDATAEEEDM 108633
Score = 28.9 bits (63), Expect(2) = 2e-33, Method: Compositional matrix adjust.
Identities = 13/32 (41%), Positives = 18/32 (56%), Gaps = 0/32 (0%)
Frame = -2
Query 4 EIITLQAGQCGNHVGKFLWSQLAKEHAIGTDG 35
EI+ L G CGN +G W ++ EH I +G
Sbjct 110001 EIVHL*TG*CGN*IGAKFWEVISDEHGIDPNG 109906
> scaffold-157
Length=706690
Score = 29.6 bits (65), Expect = 4.0, Method: Compositional matrix adjust.
Identities = 13/38 (34%), Positives = 22/38 (58%), Gaps = 0/38 (0%)
Frame = +3
Query 96 SDGASAGNSWANGYDIGTRNQDDILNKIDKEIDSTDNF 133
++GA+ S A+GY G +Q +LN +D + S +N
Sbjct 263277 AEGATLIGSTADGYSYGVYSQRQVLNLVDMYLRSMNNL 263390
Lambda K H a alpha
0.317 0.133 0.387 0.792 4.96
Gapped
Lambda K H a alpha sigma
0.267 0.0410 0.140 1.90 42.6 43.6
Effective search space used: 2892629495
Database: X5.fasta
Posted date: Nov 10, 2021 1:52 PM
Number of letters in database: 23,962,143
Number of sequences in database: 1,868
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40