TBLASTN 2.8.1+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: X5.fasta
           1,868 sequences; 23,962,143 total letters



Query= PGK_NEUCR P38667 Phosphoglycerate kinase (2.7.2.3)

Length=418
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  scaffold-693                                                        536     6e-173
  scaffold-157                                                        522     3e-168


> scaffold-693
Length=1268102

 Score = 536 bits (1380),  Expect = 6e-173, Method: Compositional matrix adjust.
 Identities = 272/442 (62%), Positives = 329/442 (74%), Gaps = 25/442 (6%)
 Frame = -1

Query  1        MSLSNKLSIEDVDLKGKRVLIR-----------------------VDFNVPLDAEKKVTN  37
                M + NKL ++D+D+KGKRVL+R                       VDFNVP  A+ K++N
Sbjct  1173191  MKIGNKLGLKDIDVKGKRVLMR*VDSHLN*C*LINIIHVLY*TCSVDFNVPF-ADGKISN  1173015

Query  38       PQRIAGAIPTIKYALDHGAKAVVLMSHLGRPDGKPNPKYSLKPVVPELEKLLGKKVTFAP  97
                 QRI  AIP+I++ L +GAKAVVLMSHLGRPDGK   KYSLKPV  E+E+LL +KVTF  
Sbjct  1173014  NQRIVAAIPSIEHCLKNGAKAVVLMSHLGRPDGKVVSKYSLKPVAAEVERLLKRKVTFLE  1172835

Query  98       DCVGPEVEEIVNKADNGEVILLENLRFHIEEEGKGTDAEGNKVKADKAKVEEFRKNLTKL  157
                DCVG +VE+    A NGEVILLENLRFHIEEEG   D  GNKVKA+K  VE+FR++L++L
Sbjct  1172834  DCVGQKVEDQCKNAANGEVILLENLRFHIEEEGSVKDEAGNKVKANKEDVEKFRQSLSQL  1172655

Query  158      GDVYINDAFGTAHRAHSSMVGIDLPVKAAGFLMKKELQYFAKVLESPQRPFLSILGGAKV  217
                GDVY+NDAFGTAHRAHSS+VGI L  +AAGFLMKKEL +F   LE+PQRPFL+I+GGAKV
Sbjct  1172654  GDVYVNDAFGTAHRAHSSVVGIKLETRAAGFLMKKELDFFGMALENPQRPFLAIIGGAKV  1172475

Query  218      SDKIQLIDNLLDKVNTLIVCGGMAFTFKKTLQNMPIGNSLFDEAGAKIVPDLVKKAEKNN  277
                SDKIQLI+NLLDKVN +I+ GGMAFTFKK +  + IG SLFDE G KIV  LV+KA+  N
Sbjct  1172474  SDKIQLIENLLDKVNAMIIGGGMAFTFKKVVDGVSIGKSLFDEEGGKIVKKLVEKAKSKN  1172295

Query  278      VKLVLPVDFTIADKFDKDANTGYATDKDGIPDGWMGLDCGEESVKLFTQAINESQTILWN  337
                V+LV PVD+  A KF KDA  GYA DK GI D WMGLD G ESVK F + + +S+TILWN
Sbjct  1172294  VELVFPVDYVTASKFAKDAEVGYADDKSGIKDEWMGLDVGSESVKKFKEVVLKSKTILWN  1172115

Query  338      GPAGVFEFDKFAKGTKATLDACVKA-AEEGRTVIIGGGDTATVAAKYGVEDKLSHVSTGG  396
                GP GVFEFD F+ GT+  LDA ++A A  G   I+GGGDTAT  +K+  +DK+SHVSTGG
Sbjct  1172114  GPMGVFEFDSFSAGTRGVLDAVIEATA*NGAISIVGGGDTATAVSKWNADDKVSHVSTGG  1171935

Query  397      GASLELLEGKALPGVVALSERQ  418
                G+S+EL EGK LPGVV L + +
Sbjct  1171934  GSSVELAEGKELPGVVNLCDSK  1171869


> scaffold-157
Length=706690

 Score = 522 bits (1345),  Expect = 3e-168, Method: Compositional matrix adjust.
 Identities = 269/440 (61%), Positives = 325/440 (74%), Gaps = 25/440 (6%)
 Frame = -3

Query  1       MSLSNKLSIEDVDLKGKRVLIR-----------------------VDFNVPLDAEKKVTN  37
               M + NKL ++D+D+KGKRVL+R                       VDFNVP  A+ K++N
Sbjct  224402  MKIGNKLGLKDIDVKGKRVLMR*VDNHLN*C*LMNIIHVLY*TCSVDFNVPF-ADGKISN  224226

Query  38      PQRIAGAIPTIKYALDHGAKAVVLMSHLGRPDGKPNPKYSLKPVVPELEKLLGKKVTFAP  97
                QRI  AIP+I++ L +GAKAVVLMSHLGRPDGK   KYSLKPV  E+E+LL +KVTF  
Sbjct  224225  NQRIVAAIPSIEHCLKNGAKAVVLMSHLGRPDGKVVSKYSLKPVAAEVERLLKRKVTFLE  224046

Query  98      DCVGPEVEEIVNKADNGEVILLENLRFHIEEEGKGTDAEGNKVKADKAKVEEFRKNLTKL  157
               DCVG +VE+    A NGEVILLENLRFHIEEEG   D  GNK KA+K  VE+FR++L++L
Sbjct  224045  DCVG*KVEDQCKNAANGEVILLENLRFHIEEEGSVKDESGNKAKANKEDVEKFRQSLSQL  223866

Query  158     GDVYINDAFGTAHRAHSSMVGIDLPVKAAGFLMKKELQYFAKVLESPQRPFLSILGGAKV  217
               GDVY+NDAFGTAHRAHSS+VGI L  +AAGFLMKKEL +F   LE+PQRPFL+I+GGAKV
Sbjct  223865  GDVYVNDAFGTAHRAHSSVVGIKLETRAAGFLMKKELDFFGMALENPQRPFLAIIGGAKV  223686

Query  218     SDKIQLIDNLLDKVNTLIVCGGMAFTFKKTLQNMPIGNSLFDEAGAKIVPDLVKKAEKNN  277
               SDKI LI+NLLDKVN +I+ GGMAFTFKK +  + IG SLFDE G KIV  LV+KA+  N
Sbjct  223685  SDKI*LIENLLDKVNAMIIGGGMAFTFKKVVDGVSIGKSLFDEEGGKIVKKLVEKAKSKN  223506

Query  278     VKLVLPVDFTIADKFDKDANTGYATDKDGIPDGWMGLDCGEESVKLFTQAINESQTILWN  337
               V+LV PVD+  A KF KDA  GYA DK GI D  MGLD G ESVK F + + +S+TILWN
Sbjct  223505  VELVFPVDYVTASKFAKDAEVGYADDKSGIKDELMGLDVGSESVKKFKEVVLKSKTILWN  223326

Query  338     GPAGVFEFDKFAKGTKATLDACVKA-AEEGRTVIIGGGDTATVAAKYGVEDKLSHVSTGG  396
               GP GVFEFD F+ GT+  LDA ++A A  G   I+GGGDTAT  +K+  +DK+SHVSTGG
Sbjct  223325  GPMGVFEFDSFSAGTRGVLDAVIEATA*NGAISIVGGGDTATAVSKWNADDKVSHVSTGG  223146

Query  397     GASLELLEGKALPGVVALSE  416
               G+S+EL EGK LPGVV L +
Sbjct  223145  GSSVELAEGKELPGVVNLCD  223086



Lambda      K        H        a         alpha
   0.315    0.136    0.386    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 2472192021


  Database: X5.fasta
    Posted date:  Nov 15, 2021  9:19 AM
  Number of letters in database: 23,962,143
  Number of sequences in database:  1,868



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40