Rubredoxin description
# | Atoms | Bond's length, Å | Angle N-O-C, ° |
---|---|---|---|
Alpha-helix | |||
1 | N(048LEU)-O(044LEU) | 2,76 | 160,6 |
2 | N(049ALA)-O(045THR) | 3,04 | 149,3 |
3 | N(052GLU)-O(048LEU) | 2,86 | 153,6 |
Beta-layer | |||
1 | N(050GLU)-O(005THR) | 2,99 | 153,2 |
2 | N(005THR)-O(050GLU) | 2,76 | 156,5 |
3 | N(013TYR)-O(004TYR) | 2.71 | 167,3 |
*This protein is required because the initial one doesn't alpha-helixes of sufficient lengths (everything is obscure in it). The PDB entry is 3TYS.
Different sources provide various information about the hydrogen bond length and N-O-C angle. The most recurrent parameters found in literature are 2.9 Å and 135°. The acquired data perform deviation from the latter. The medium hydrogen bond's length is 2,89 Å for alpha-helix and 2,82 Å for beta-layer, both values differ from the standard by 0,01 Å and 0,08 Å respectively. The medium angle in alpha-helix is 154,5° and in beta-layer it is 159,0°. So the difference is 19,5° and 24°. Should one be amazed?! –It can not be said for sure because the statistical tests are not applicable in such cases.
Sources:
Lehninger Principles of Biochemistry (Russian edition)