MAST version 4.3.0 (Release date: Sat Sep 26 01:51:56 PDT 2009)

For further information on how to interpret these results or to get a copy of the MAST software please access http://meme.nbcr.net.

If you use this program in your research, please cite:

Timothy L. Bailey and Michael Gribskov, "Combining evidence using p-values: application to sequence homology searches", Bioinformatics, 14(48-54), 1998.

	DATABASE ./seed.fasta (peptide)
	Last updated on Wed May 30 05:35:26 2012
	Database contains 243 sequences, 19751 residues

	MOTIFS ./memeout.txt (peptide)
	MOTIF WIDTH BEST POSSIBLE MATCH
	----- ----- -------------------
	  1    38   YCFEELELHRIMICCMVDNPKSNAVPERNGFEKEGCAK
	  2    21   NHKCHIGYWIGKKWQGKGIMT
	  3    21   YYNENRHHLKPWEPVRDPSHC

	PAIRWISE MOTIF CORRELATIONS:
	MOTIF     1     2
	----- ----- -----
	   2   0.14
	   3   0.16  0.11
	No overly similar pairs (correlation > 0.60) found.

	Random model letter frequencies (from non-redundant database):
	A 0.073 C 0.018 D 0.052 E 0.062 F 0.040 G 0.069 H 0.022 I 0.056 K 0.058 
	L 0.092 M 0.023 N 0.046 P 0.051 Q 0.041 R 0.052 S 0.074 T 0.059 V 0.064 
	W 0.013 Y 0.033 

• Each of the following 53 sequences has E-value less than 10.
• The E-value of a sequence is the expected number of sequences in a random database of the same size that would match the motifs as well as the sequence does and is equal to the combined p-value of the sequence times the number of sequences in the database.
• The combined p-value of a sequence measures the strength of the match of the sequence to all the motifs and is calculated by
  • finding the score of the single best match of each motif to the sequence (best matches may overlap),
  • calculating the sequence p-value of each score,
  • forming the product of the p-values,
  • taking the p-value of the product.
• The sequence p-value of a score is defined as the probability of a random sequence of the same length containing some match with as good or better a score.
• The score for the match of a position in a sequence to a motif is computed by by summing the appropriate entry from each column of the position-dependent scoring matrix that represents the motif.
• Sequences shorter than one or more of the motifs are skipped.
• The table is sorted by increasing E-value.

• The ordering and spacing of all non-overlapping motif occurrences are shown for each high-scoring sequence listed in Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001.
• The POSITION p-value of a match is the probability of a single random subsequence of the length of the motif scoring at least as well as the observed match.
• For each sequence, all motif occurrences are shown unless there are overlaps. In that case, a motif occurrence is shown only if its p-value is less than the product of the p-values of the other (lower-numbered) motif occurrences that it overlaps.
• The table also shows the E-value of each sequence.
• Spacers and motif occurences are indicated by
• Spacers and motif occurences are indicated by
• Spacers and motif occurences are indicated by

  • n

    occurrence of motif `n' with p-value less than 0.0001.

  • 1

    Sequences longer than 1000 are not shown to scale and are indicated by thicker lines.

• The positions and p-values of the non-overlapping motif occurrences are shown above the actual sequence for each of the high-scoring sequences from Section I.
• A motif occurrence is defined as a position in the sequence whose match to the motif has POSITION p-value less than 0.0001 as defined in Section II.
• For each sequence, the first line specifies the name of the sequence.
• The second (and possibly more) lines give a description of the sequence.
• Following the description line(s) is a line giving the length, combined p-value, and E-value of the sequence as defined in Section I.
• The next line reproduces the motif diagram from Section II.
• The entire sequence is printed on the following lines.
• Motif occurrences are indicated directly above their positions in the sequence on lines showing
  • the motif number of the occurrence,
  • the position p-value of the occurrence,
  • the best possible match to the motif, and
  • columns whose match to the motif has a positive score (indicated by a plus sign).

                         [2]
                         1.3e-21
                         NHKCHIGYWIGKKWQGKGIMT
                         +++++++++++++++++++++
1    DPDEKEIIGVANFSNVVRGSFHACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLL

                        [2]
                        6.4e-20
                        NHKCHIGYWIGKKWQGKGIMT
                        +++++++++++++++++++++
1    IFKEDELIGVISFNRIEPLNKTAEIGYWLDESHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVAL

                        [2]
                        2.1e-20
                        NHKCHIGYWIGKKWQGKGIMT
                        +++++++++++++++++++++
1    LLYDGSLCGMISLHNLDQVNRKAEIGYWIAKEFEGKGIITAACRKLITYAFEELELNRVAICAAVGNEKSRAVPE

                        [2]
                        8.2e-16
                        NHKCHIGYWIGKKWQGKGIMT
                        ++++++++++++ ++++++ +
1    IKYKTKIAGVVSFNIIDHANKTAYIGYWLGANFQGKGIVTNAINKLIQEYGDSGVIKRFVIKCIVDNKKSNATAL

                         [2]
                         3.0e-12
                         NHKCHIGYWIGKKWQGKGIMT
                          +++ +++ +++++ ++++++
1    TASDDRLIGTVSLFQIIRGALQTAFIGYFLDKAHNGKGIMTEAVRLVVDYAFHELKLHRIEAGVMPRNLGSMRVL

                           [2]
                           1.1e-07
                           NHKCHIGYWIGKKWQGKGIMT
                           +++++    ++++  +++ ++
1    EDENGNVAAWISFETFYGRPAYNKTAEVSIYIDEACRGKGVGSYLLQEALRIAPNLGIRSLMAFIFGHNKPSLKL

                      [2]
                      1.0e-07
                      NHKCHIGYWIGKKWQGKGIMT
                         ++ ++   ++++++++++
1    ALLGDKIVGHAVYRKTSGDSAEVGFVVREEFQGRGLGTILLGALAEIAEKRGIKIFEADVRADNYAIIEVFKESG

                           [2]
                           4.0e-07
                           NHKCHIGYWIGKKWQGKGIMT
                           +  ++++  +++++  +++
1    LKETQELIGFVGLNIPNAPLPFNPCVEIGWRIAQSHWRKGYTYEAALTVLKYAFEQLKLEEVVAFTAVTNLPSEG

                        [2]
                        3.2e-07
                        NHKCHIGYWIGKKWQGKGIMT
                         + +++ + ++  + +++++
1    LKTDNKLIGTAGFNIINLEHHRAEISYDLDPNFWGQGIMLKSIKNILKFADCIGIIRVQATVITDNFRSVNLLER

                        [2]
                        2.7e-07
                        NHKCHIGYWIGKKWQGKGIMT
                        +  +++++ ++  + +++  +
1    VKETDEVIGTCGFNMIDQENGRAEIGYDLGRNHWGKGFASEAVQKLIDYGFTSLNLNRIEAKVEPENTPSIKLLN

                             [2]
                             4.1e-06
                             NHKCHIGYWIGKKWQGKGIMT
                                +   +  ++++++++++
1    AKVGDKIVGFIVCDKDWFSKYEGRIVGAIHEFVVDKKFQGKGIGRKLLITCLDFLGKYNDTIELWVGEKNYGAMN

                           [2]
                           2.1e-07
                           NHKCHIGYWIGKKWQGKGIMT
                             +++    +++++ ++++++
1    AVENGVLQGFACSQKYRAIPAFDDTVEVSVYLAQEAKGKGLGSKLYTQLFSSIRAYGVHRILSGVALPNDASVAL

                        [2]
                        2.8e-07
                        NHKCHIGYWIGKKWQGKGIMT
                        +  ++++ ++  +  ++++ +
1    LWLDGTLVGGLLYRVFDAATGVCEIGCWLEPAGTGRGLVTRGARVLIDWAFDERGMHRVEWYASTANTPSANAAR

                          [2]
                          7.4e-06
                          NHKCHIGYWIGKKWQGKGIMT
                           + + +    ++++  +++++
1    AVYNGNVAGVATLVLNPYVKVSHHALISIIVGEEFRNKGIGTALLNNLIHLAKTRFKLEVLYLEVYEGNPALHLY

                                             [3]
                                             8.7e-06
                                             YYNENRHHLKPWEPVRDPSHC
                                               ++ ++++++    +
1    DVVSGEQVGYARVVTDLATFAWLCDVYVDPSVRAKGLGTALVAAVREHLAPHGLRRILLATHDAHGVYGKLGFT

                        [2]
                        3.4e-06
                        NHKCHIGYWIGKKWQGKGIMT
                         + ++    +  +++++++ +
1    VECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYR

                              [2]
                              1.2e-06
                              NHKCHIGYWIGKKWQGKGIMT
                               +     + +++++++++++
1    LYYEGDLVGFAMYGLFPEYDEDNKNGRVWLDRFFIDERYQGKGLGKKMLKALIQHLAELYKCKRIYLSIFENNIH

                       [2]
                       2.1e-06
                       NHKCHIGYWIGKKWQGKGIMT
                       +++++       +++ +++++
1    IESQGEILAVGRLSKLHGTKTAEFAMLVSDRYQCQGLGAELLRRLLQIGRDEQIERITADILADNYGMQRVCEKL

                        [2]
                        2.6e-05
                        NHKCHIGYWIGKKWQGKGIMT
                        +   ++++   +++  +++
1    KKDNYEIVGGTGFLGDPDENGMIEIGFATNKSHRRKGYCVEAAQKLINWALSRETVSRITARCEHDNLGSQKTLE

                       [2]
                       2.4e-05
                       NHKCHIGYWIGKKWQGKGIMT
                        ++ +++      ++ +++ +
1    LRDEDCLIGHIVFYKYFGEHTYEIGWVFNPNYQNKGYASEAAQAILEYGFKEMNLHRIIATCQPENIPSYRVMKK

                         [2]
                         3.3e-05
                         NHKCHIGYWIGKKWQGKGIMT
                         +    +++   + + +++++
1    VPDGGSIAGFVNINNIVEGGFLSGALGYGAFAHAAGRGLMREGLDLVVRYAFGPMRLHRLEINVQPGNAASIALA

                        [2]
                        2.9e-05
                        NHKCHIGYWIGKKWQGKGIMT
                         + +++++ +  ++   +  +
1    RRDTKELIGTIGFHALAQKHRRAEIGYEIIPEHWRNGFASEVISKVVSYGFSALGLSRIGAVVFTDNEASNRLLL

      [2]
      1.5e-05
      NHKCHIGYWIGKKWQGKGIMT
      + +  +   +++++ + ++ +
1    RTVAETIVGTIDLRFDGEGLASGQVNVAYGLYPSWRGRGLATRAVDLVCQYAAEHGATEAVIKVEPENSASARVA

                           [2]
                           2.0e-05
                           NHKCHIGYWIGKKWQGKGIMT
                            +++++   ++  +  ++++
1    VTEHKQIVGWLGFQRFYGRPAYHRTAELSIYLDPLWHHRGLGQYLLKTAIAKAPQLGLKTLLGYIFAHNQPSIRL

                       [2]
                       7.8e-05
                       NHKCHIGYWIGKKWQGKGIMT
                         +  +++ +++  +  ++ +
1    WTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEA

                         [2]
                         2.3e-05
                         NHKCHIGYWIGKKWQGKGIMT
                          + + ++++   ++ + +  +
1    IELDGQFCGQLTIGNVTHGALRSAWIGYWVPSAATGGGVATGALALGLDHCFGPVMLHRVEATVRPENAASRAVL

                           [2]
                           3.0e-05
                           NHKCHIGYWIGKKWQGKGIMT
                            ++++        +++++++
1    SEENGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHL

                      [2]
                      1.7e-05
                      NHKCHIGYWIGKKWQGKGIMT
                        ++++   + + ++ ++++
1    AEKDGRIVGHLVIVPTEDMKVDLTIFIHQDYQNRGLGQEMMKLIIDYCKKAGFKGIMLVTERSNARAIHVYKKMG

                                      [2]
                                      3.9e-05
                                      NHKCHIGYWIGKKWQGKGIMT
                                             +  + +++++++++
1    YQKDNRIIGTIALVYKRIKEKGIWWVPEELMNEKVGLIEFFVVDPEFQGKGIGSTLLEFAVKRLRSLGKDPYVVT

CPU: kodomo.fbb.msu.ru
Time 0.012000 secs.

mast ./memeout.txt -d ./seed.fasta -ev 10.000000 -mt 0.000100