FastQCFastQC Report
Вт 5 дек 2017
SRR4240356.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240356.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7511529
Sequences flagged as poor quality0
Sequence length36
%GC29

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGAAA334070.4447430077152068Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTGGAT278370.3705903285469576Illumina Single End Adapter 1 (100% over 33bp)
GATCGGAAGAGCTCGTATGCCGTCTTCTGCTTAGAT216700.2884898667102264Illumina Single End Adapter 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG149800.028.900879
AGAGCTC151950.028.5133278
CGTATGC147300.028.04874214
GCTCGTA150800.028.01728811
TGCCGTC141850.027.92392218
AGCTCGT150900.027.9166610
GCCGTCT142450.027.77581819
TCGTATG152650.027.65028613
ATGCCGT144050.027.63716917
GTATGCC149200.027.54098915
TATGCCG149950.027.37010416
CTCGTAT154550.027.36674312
GAAGAGC160150.027.2816316
AAGAGCT161000.027.0001817
CCGTCTT147050.026.9484120
CGTCTTC148800.026.57005121
GGAAGAG168700.026.5109835
CGGAAGA171000.026.011244
CTGCTTG95350.025.88634327
GCTTGGA41750.025.74536529
Produced by FastQC (version 0.11.2)