BLASTX 2.2.10 [Oct-19-2004]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= KPN2Jun2003
(7000 letters)
Database: sal
4429 sequences; 1,395,779 total letters
Searching.........done
Score E
Sequences producing significant alignments: (bits) Value
Q8ZMC0 Q8ZMC0_SALTY Membrane-bound lytic murein transglycosylase... 718 0.0
Q8ZMC1 Q8ZMC1_SALTY Putative enzyme. 146 2e-35
Q93IS1 Q93IS1_SALTY Putative cytoplasmic protein. 113 2e-25
Q7CPI5 Q7CPI5_SALTY Putative outer membrane lipoprotein. 59 6e-09
Q9L9I9 Q9L9I9_SALTY Thiamin biosynthesis protein, thiazole moiety. 53 3e-07
Q56067 MOEB_SALTY Molybdopterin biosynthesis protein moeB. 50 2e-06
P02936 OMPA_SALTY Outer membrane protein A precursor (Outer memb... 50 2e-06
Q93IS0 Q93IS0_SALTY Putative outer membrane protein, OmpA family. 41 0.001
>Q8ZMC0 Q8ZMC0_SALTY Membrane-bound lytic murein transglycosylase A
(EC 3.2.1.-).
Length = 365
Score = 718 bits (1854), Expect = 0.0
Identities = 344/365 (94%), Positives = 356/365 (97%)
Frame = -3
Query: 1409 MKGRWAKYVATGAMLAMLAACSSKPTDRGQQYSDGKFTQPFSLVNQPDAVGAPINAGDFS 1230
MKGRWAKYVATG MLAMLAACSSKPTDRGQQY DGKFTQPFSLVNQPDAVGAPINAGDF+
Sbjct: 1 MKGRWAKYVATGVMLAMLAACSSKPTDRGQQYKDGKFTQPFSLVNQPDAVGAPINAGDFA 60
Query: 1229 EQVNQIRNASPRLYNSQSNVYNALQEWLRAGGDTRTLRQFGIDAWQMQGVDNYGNVQFTG 1050
EQVNQIR+ASPRLY +QSNVYNA+Q WLR+GGDTRT+RQFGIDAWQM+G DNYGNVQFTG
Sbjct: 61 EQVNQIRSASPRLYTNQSNVYNAVQNWLRSGGDTRTMRQFGIDAWQMEGTDNYGNVQFTG 120
Query: 1049 YYTPVVQARHTRQGEFQYPIYRMPPKRGKLPSRASIYAGALSDNYVLAYSNSLMDNFIMD 870
YYTPVVQARHTRQG FQYPIY MPPKRG+LPSRA IYAGALSD Y+LA+SNSLMDNFIMD
Sbjct: 121 YYTPVVQARHTRQGAFQYPIYSMPPKRGRLPSRAQIYAGALSDKYILAWSNSLMDNFIMD 180
Query: 869 VQGSGYIDFGDGSPLNFFSYAGKNGWPYRSIGKVLIDRGEVKKEDMSMQAIREWGEKHSE 690
VQGSGYIDFGDGSPLNFFSYAGKNGWPYRSIGKVLIDRGEVKKEDMSMQAIREWGEKHSE
Sbjct: 181 VQGSGYIDFGDGSPLNFFSYAGKNGWPYRSIGKVLIDRGEVKKEDMSMQAIREWGEKHSE 240
Query: 689 AEVRELLEQNPSFVFFKPQSFAPVKGASAVPLIGRASVASDRSIIPPGTTLLAEVPLLDN 510
AEVRELLEQNPSFVFFKPQSFAPVKGASAVPLIGRASVASDRSIIPPGTTLLAEVPLLDN
Sbjct: 241 AEVRELLEQNPSFVFFKPQSFAPVKGASAVPLIGRASVASDRSIIPPGTTLLAEVPLLDN 300
Query: 509 NGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIGPDAGHRAGWYNHYGRVWVLKNAPGAG 330
NGKF+GQYELRLMVALDVGGAIKGQHFDIYQGIGPDAGHRAGWYNHYGRVWVLK+APGAG
Sbjct: 301 NGKFSGQYELRLMVALDVGGAIKGQHFDIYQGIGPDAGHRAGWYNHYGRVWVLKSAPGAG 360
Query: 329 NVFSG 315
NVFSG
Sbjct: 361 NVFSG 365
>Q8ZMC1 Q8ZMC1_SALTY Putative enzyme.
Length = 268
Score = 146 bits (369), Expect = 2e-35
Identities = 71/74 (95%), Positives = 73/74 (98%)
Frame = -3
Query: 224 MSVVISDAWRQRFGGTARLYGEKALQCFADAHVCVVGIGGVGSWAAEALARTGIGAITLI 45
MSVVISDAWRQRFGGTARLYGEKALQ FA+AH+CVVGIGGVGSWAAEALARTGIGAITLI
Sbjct: 1 MSVVISDAWRQRFGGTARLYGEKALQRFAEAHICVVGIGGVGSWAAEALARTGIGAITLI 60
Query: 44 DMDDVCVTNTNRQI 3
DMDDVCVTNTNRQI
Sbjct: 61 DMDDVCVTNTNRQI 74
>Q93IS1 Q93IS1_SALTY Putative cytoplasmic protein.
Length = 447
Score = 113 bits (282), Expect = 2e-25
Identities = 114/448 (25%), Positives = 188/448 (41%), Gaps = 9/448 (2%)
Frame = +1
Query: 3424 RPLWAEGTFLSSQQFQQQARWETYSNNSIAQLGIRHPWGVARVEFDREALALGKLKLRTL 3603
R +W+EG FL Q FQQQ R+ + + + WG + D EAL +GKL L+
Sbjct: 6 RVVWSEGQFLLPQMFQQQERYLEHVMHYRSLPLTPFFWGFSHYNIDGEALNIGKLILKEA 65
Query: 3604 RLRFSDGSLIDSEVSDALPLACDLHTLAVENTAIVVLALPLEHANGGNLGQEKHTERPLR 3783
F DG+ ++ D PL L L + LA+P+ N + + E R
Sbjct: 66 SGIFPDGTPFNAP--DHTPLPPPLTILPEHLNQQICLAVPVRAPNSEETTFDNNPESLAR 123
Query: 3784 YRQEWHKVQDIYGSDSEEMAVECHALSLRFAHDN--NQDYITCPLARLTR-NGQGNWSQD 3954
+ H ++D ++ L LR + +I PL R+T N G D
Sbjct: 124 FSVHEHDIRDANSLGRGAQLLQLSHLRLRLLPEKAVTGAWIGLPLTRITGLNPDGRIDID 183
Query: 3955 ESYIPPLLALSAHIGLVERL----DTLLLQLQAKCRRLMALRRESNQRMADFAVADVSLF 4122
IPP++ A + L D + ++ + RL ++ A+VS +
Sbjct: 184 HDLIPPIINYQASSLMCTWLSWINDLIRMRADSLAERLTGSDNHGHE------AAEVSDY 237
Query: 4123 WLLNALNSAEPVLSDFLRYPSIHPELVWRELARLAGALLTF--SLEHNVSAIPAYVHDAP 4296
LL LN EP+L+ + P + PE+++R L+ LAG L T+ + Y H P
Sbjct: 238 LLLQILNRFEPLLTHLAKTP-LAPEVLYRYLSELAGELSTYVRPQTRRPAEYKEYKHLTP 296
Query: 4297 ASVFPPLFTLLSELLEASLPSRVIALELESLPGNRWKAELHDPRLREEADFYLSVRSSLP 4476
+ L + LL A L +EL+ A + L + + L++++S+P
Sbjct: 297 YAGLKSLVDEVQFLLNAVLIRGAQRIELKEGTYGILNAVVAPSDLADFSTLVLAIKASMP 356
Query: 4477 THVVLQQLPRLCKIGAPDDVTLLVNVALNGVPVVPLTQVPAALPLRLENQYFALDMQSAP 4656
T V+LQ KIG D + L+ L G+ + L P +P + Y+ + + A
Sbjct: 357 TDVLLQHFAAQTKIGPSDRLPELIRSHLPGLALQVLPVPPRQIPFQAGYIYYDIRREGAL 416
Query: 4657 AKAMLDAGCCMIYAPGTLGDLKPELFAV 4740
+ + G ++ G L+ EL+ V
Sbjct: 417 WEHIARYGGMAMHTAGEFPGLETELWGV 444
>Q7CPI5 Q7CPI5_SALTY Putative outer membrane lipoprotein.
Length = 220
Score = 58.5 bits (140), Expect = 6e-09
Identities = 31/93 (33%), Positives = 50/93 (53%)
Frame = +2
Query: 6719 FDVGQARLKDGSTKVLINALVNIRARPGWLIVVTGYTDTTGNKKANQQLSLRRAEAVRDW 6898
FD A LK L + ++ P + V GYTD+TG+ N +LS +RA++V
Sbjct: 116 FDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVVGYTDSTGSHDLNMRLSQQRADSVASS 175
Query: 6899 MLQTSDIPATFFAVQGLGESHPAATNDTPEGRA 6997
++ T + A+ G+G ++P A+N T EG+A
Sbjct: 176 LI-TQGVDASRIRTSGMGPANPIASNSTAEGKA 207
>Q9L9I9 Q9L9I9_SALTY Thiamin biosynthesis protein, thiazole moiety.
Length = 252
Score = 52.8 bits (125), Expect = 3e-07
Identities = 30/65 (46%), Positives = 42/65 (64%)
Frame = -3
Query: 197 RQRFGGTARLYGEKALQCFADAHVCVVGIGGVGSWAAEALARTGIGAITLIDMDDVCVTN 18
RQ G + G++ L ++HV +VG+GG+GS AA LA GIG +TL+D DDV ++N
Sbjct: 11 RQILLGDIAIEGQQKL---LNSHVLIVGLGGLGSPAALYLAGAGIGKLTLVDDDDVHLSN 67
Query: 17 TNRQI 3
RQI
Sbjct: 68 LQRQI 72
>Q56067 MOEB_SALTY Molybdopterin biosynthesis protein moeB.
Length = 249
Score = 50.4 bits (119), Expect = 2e-06
Identities = 26/53 (49%), Positives = 36/53 (67%)
Frame = -3
Query: 164 GEKALQCFADAHVCVVGIGGVGSWAAEALARTGIGAITLIDMDDVCVTNTNRQ 6
G++AL+ DA V VVG+GG+G A + LA G+G +TL+D D V V+N RQ
Sbjct: 25 GQEALK---DARVLVVGLGGLGCAATQYLAGAGVGQLTLLDFDTVSVSNLQRQ 74
>P02936 OMPA_SALTY Outer membrane protein A precursor (Outer membrane
protein 33K) (Outer membrane major heat-modifiable
protein).
Length = 350
Score = 50.1 bits (118), Expect = 2e-06
Identities = 36/105 (34%), Positives = 59/105 (56%), Gaps = 5/105 (4%)
Frame = +2
Query: 6701 LDSMALFDVGQARLKDGSTKVL---INALVNIRARPGWLIVVTGYTDTTGNKKANQQLSL 6871
L S LF+ ++ LK + L + L N+ + G +VV G+TD G+ NQ LS
Sbjct: 221 LKSDVLFNFNKSTLKPEGQQALDQLYSQLSNLDPKDG-SVVVLGFTDRIGSDAYNQGLSE 279
Query: 6872 RRAEAVRDWMLQTSDIPATFFAVQGLGESHPAATN--DTPEGRAA 7000
+RA++V D+++ + IP+ + +G+GES+P N D + RAA
Sbjct: 280 KRAQSVVDYLI-SKGIPSDKISARGMGESNPVTGNTCDNVKPRAA 323
>Q93IS0 Q93IS0_SALTY Putative outer membrane protein, OmpA family.
Length = 434
Score = 41.2 bits (95), Expect = 0.001
Identities = 33/99 (33%), Positives = 49/99 (49%), Gaps = 4/99 (4%)
Frame = +2
Query: 6713 ALFDVGQARLKDGSTKVLINALVNIRARPGWLIVVTGYTDT----TGNKKANQQLSLRRA 6880
A+F GQ + D V+ A I AR G + VTG+TD+ + +N LS +RA
Sbjct: 327 AMFVPGQKTVSDAIRPVINKAAREI-ARVGGAVTVTGHTDSQPIHSAEFPSNLVLSEKRA 385
Query: 6881 EAVRDWMLQTSDIPATFFAVQGLGESHPAATNDTPEGRA 6997
V +L + +PA + G G++ P A N + GRA
Sbjct: 386 AEVAA-LLTSGGVPAGRVHIVGKGDTVPVADNGSKAGRA 423
Database: sal
Posted date: Oct 3, 2006 6:42 PM
Number of letters in database: 1,395,779
Number of sequences in database: 4429
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 20,002,811
Number of Sequences: 4429
Number of extensions: 486016
Number of successful extensions: 1500
Number of sequences better than 1.0e-03: 8
Number of HSP's better than 0.0 without gapping: 1183
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1494
length of database: 1,395,779
effective HSP length: 100
effective length of database: 952,879
effective search space used: 2126825928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)