FastQCFastQC Report
Вс 2 дек 2018
cleaned.40.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamecleaned.40.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17491
Sequences flagged as poor quality0
Sequence length45-51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCTT1190.680349894231319No Hit
GTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTGC730.4173574981419016No Hit
CCCTGATGCACCACAGTACTAATGCAATTCCTGAGAACTGGCCCTTTTGCT680.38877136813218227No Hit
CTGGGAGTCATTATCTGTCTCACTTTCTGGATCTCTGTAATTATCATGAAT620.35446801212051915No Hit
CCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGA610.3487507861185753No Hit
CTTCGTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTT570.3258818821107999No Hit
GGGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGC570.3258818821107999No Hit
GTCGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTC530.3030129781030244No Hit
CTGTAATGCCAGCACCGCGGGGCTGCTGCTCAGGACGCATTCCTGCCCCAG500.28586130009719285No Hit
CTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATATTTTA480.27442684809330514No Hit
GGGGTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGC470.2687096220913613No Hit
GCCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGT460.26299239608941744No Hit
CTGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAAT450.2572751700874736No Hit
CCCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTA440.2515579440855297No Hit
CTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCA440.2515579440855297No Hit
CTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGATCTGA430.24584071808358585No Hit
CCCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAG420.240123492081642No Hit
GGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTAG410.23440626607969814No Hit
CTTTAAATGTAGATCCAGCTTTGTGTCAGCAAGTAGACTAATATATGTTGT390.22297181407581043No Hit
CTGGTATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATT390.22297181407581043No Hit
CCATCTTCTTGGTGATTTGAGTATCAAAGGTAGATTCATTTAGCTTTGTAA370.21153736207192272No Hit
CTCCCTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAG360.20582013606997884No Hit
CTCAATTAATCCTAACCTGGAGGTCATGGGCACAACGCTATAGGTCCTCAG350.200102910068035No Hit
GTCCCTCTGGGCCACGGCCTGTAACGGGGTGATGAGGCTGCTGTGCTTGAT340.19438568406609114No Hit
GCCCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCG340.19438568406609114No Hit
CTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATCTGCTT340.19438568406609114No Hit
CTAGAAACTTTTTCATGCAGTCCACATAATTATTGAACCGCGGAGTTCCTG330.18866845806414728No Hit
CTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTGGTCTAACATGCCGT320.18295123206220343No Hit
CTGGCATCTGCGTGGCTTTGCCCAACATAAACATAACCATGCTATGTAAAA320.18295123206220343No Hit
GGGGGTATAGCTCAGGCCCAGTTTGAAAGCCATCTGCAGTGCAGGAACGTA320.18295123206220343No Hit
GTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATA320.18295123206220343No Hit
CTCTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGC310.17723400606025957No Hit
CTCCTCTCCAGCAGCTGATTCTCTCCTGCCATGTTCTCTGTAAATGCATCG310.17723400606025957No Hit
CTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTACTGTCTG310.17723400606025957No Hit
CCGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAAC310.17723400606025957No Hit
CTCTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTT300.17151678005831572No Hit
CATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAAT300.17151678005831572No Hit
GTGCTTTCCAATGTGCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACAT300.17151678005831572No Hit
CTTGTTTCTCAGGGAGAATCTCTCTGCAAAATTCCACCAGGTTAATGAAAG300.17151678005831572No Hit
CCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAA290.16579955405637187No Hit
GTTCCTATGCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCT290.16579955405637187No Hit
GTCCGCTTATAGAGCTGGTACATGGGTGGGGCTCCCTGTCCCCCTTCTGGC290.16579955405637187No Hit
GCCTTGTTTGCTTATAAAGCTGATGAACTCCTGAATTTCTGTTAAAGCCTG280.160082328054428No Hit
CTGGCAATAAAGGAACGAATTTATAAAAGAGTTCAATGGATTTGTGTCGAC280.160082328054428No Hit
CTCTGAACTCCGGTTGAGAAATCAAATTGACTCATTTCCTCACTCAGGGTA280.160082328054428No Hit
GCCACCTCTTTGCCAAATTTCACAAATAAAGCAAAGCAAGAATACTTTTCT280.160082328054428No Hit
GTGAGGAACATCTGCTTGCAGCGCTGAATGAGCTCAACGTACATGAAGTCA280.160082328054428No Hit
CTCCTTCAGTAGTTTCATAGCAAGTGAGAAATTGTTCTGCTTCCGGGCACT280.160082328054428No Hit
CTCGTTTTCTAAAAGACGTGACTGTTTCAGTACGATTAGCGCCCTTATACA280.160082328054428No Hit
GTTCCCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCA280.160082328054428No Hit
GTCCTCCTAGAGATCCAAGCATTTGTACTACTCTAATTCTTATTTCTTCTA270.15436510205248413No Hit
GGGGTTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTT260.14864787605054028No Hit
CTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCATAAA260.14864787605054028No Hit
GTCATAGGGAGGCAGGTCATTGGCCATCACGATGCCTAGCAATTGAATCCC260.14864787605054028No Hit
CTTTGAGATAATTCCAATGCATCTAGAAACTTTTTCATGCAGTCCACATAA260.14864787605054028No Hit
GGGGCTTTGAGTCTTTGTTCATTTTTAAAAGTAGCATGTTGGTAATGTCGT250.14293065004859642No Hit
GTAGAAGTATCCTTGAAGGAATAGCTTTCGCTGCTTATGATGAAGGGATAA250.14293065004859642No Hit
ATTTTTTCTAAGAAAATACATAAAAACTTTAGGATTTCTTCTCTACATTCA250.14293065004859642No Hit
CTGGTTTTTGACTCTTTATGCAGCTCCTTCAGTAGTTTCATAGCAAGTGAG250.14293065004859642No Hit
GTTTGATGAAAGGTTCTTTGTCTTCTTCAGATGCTTTAACACCACTTTATT250.14293065004859642No Hit
GCTCTGTCAACATCATCTTGCAGGAGGTAAAGCAGACTCAGCTCTTGACTG240.13721342404665257No Hit
CGCAAGTTTCAGTCCTTGGTGTTTCTGGTTTGCTCGCTCCCTGAAGCTCTG240.13721342404665257No Hit
CTCCACACGACAAAGTACCACCTCCAGACAGAGTGTTTTCAACACTCCATG240.13721342404665257No Hit
CCTGAATACAAGAGACAAAGGGTGGAAAGAAAGAGAAGGTGGTATTAAGAA240.13721342404665257No Hit
CCGCGATCACCTTCTCTGAATCCTCTGAACTGGATCCAGAAAGCTCTAAGA240.13721342404665257No Hit
GTGGGAAACTGTCTATCTGCATCACCACGAGGTGCTCCAGAACTGGAGTAT230.13149619804470872No Hit
CGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGATC230.13149619804470872No Hit
GTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCAA230.13149619804470872No Hit
GGGGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGT230.13149619804470872No Hit
CTCAGATGGGTCTCTAGGATATCTTTGTATGGGGACATCTTTAGAGCTTTC230.13149619804470872No Hit
CTCCTTGTTGTTCGAATCCACATTTCTGATGATTCCATAAAACTGCTCCAT230.13149619804470872No Hit
GGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAACATC230.13149619804470872No Hit
CGCGGAGTTCCTGGAGGAAATTCCCTGGACTGCATGGGGAAGTGAGCAACG230.13149619804470872No Hit
CTTATACATTAGCATGTAAGCTCCAACATCATGTTTTCCTGACATTTTTGT230.13149619804470872No Hit
GGCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTC230.13149619804470872No Hit
GGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAGAA230.13149619804470872No Hit
CCATGGTTCAAAAAATTCTGCTTGTTTCTCAGGGAGAATCTCTCTGCAAAA220.12577897204276486No Hit
CTGGCTTCTTTCCTAATTTCAATGTACTTTTTCTTTCTTTCCATAGGAACC220.12577897204276486No Hit
CTTTCATCATCTCTATGTCTCATGACTTGAACGAAGTCCTTGCTCTTTAAC220.12577897204276486No Hit
CTTGAAGTCTGGGGATCTTCTTCCATGGACTTAGTGAAGTTGCACAGAAGT220.12577897204276486No Hit
GCTGGATGCAAGTTAGCCGCTGGATCTGAAGTTGGGATCATCCAAACACCA220.12577897204276486No Hit
GTCGACATTCTGTCTGGGGCCTCCCACAATGAGCTAAAAGCCACTTGACCA220.12577897204276486No Hit
GTGGGCACACCACTTTAACAAGACTTCAAATAGAGACACGTAGTTGTCCAG220.12577897204276486No Hit
GGCTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCG220.12577897204276486No Hit
GTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGGG220.12577897204276486No Hit
CTCCATAGTATTCTGATGTTGCTTCAATTGTTTCGCAACCAGTTCACACAG210.120061746040821No Hit
CTTGCCACAAACTCCTTATTCTTATGACCAGTAGAAGTATCCTTGAAGGAA210.120061746040821No Hit
CTCACCTAATCTTTGATGTTACTATAACCTTATCTTTGATTTAACCCATAC210.120061746040821No Hit
CTCGTTTTTGCTCAGCAACGCCTTTTCTGGCATACATCAAACTGAGCTTCT210.120061746040821No Hit
CACGTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCC210.120061746040821No Hit
GGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAGAAAATTTGTTG210.120061746040821No Hit
CCTACATGTTGTAGAAGGATGGGAAACGAATTCCACAACGGGGTTCAGAAG210.120061746040821No Hit
GTAGTCTTTGTATGTGGGCACCTTCCATTTGCCAGTTCTGACTTCCCCTGA210.120061746040821No Hit
GTCAGAGCTCAGGAGATGTCTGAAGAGATCCACGTAGTCTTTGTATGTGGG200.11434452003887714No Hit
GGTCCATTTTAGCATCTGGATATCTGTTTGTCCAGGTGTTCAGAAGTCTCT200.11434452003887714No Hit
CCGGGCACTGTCTATCATCTTCATTTTCATGGAAAACTTGCAACTCCTGAT200.11434452003887714No Hit
CTCTCTTACTGCTGATGTCATCTGGGTCTTAAGTTCACCCAGAAAAGCGCG200.11434452003887714No Hit
GCTGCTTCTACAATGTTACATCAACTATACTTAGCTTTACTCTCCCAAAAT200.11434452003887714No Hit
CTCGAACATGGGGTTTGGATAATCTGGGCTCATGCTGGTCATTTCGAGCAG200.11434452003887714No Hit
CTGGAGAGTGCCCTGGGAGGCCTGGGTCTCCACAAACATCGGAGTGAGAAC200.11434452003887714No Hit
CTTTAGAATTAGGATCTTTACCGGAAAACTTTTCAAATATTAACCTATAAG200.11434452003887714No Hit
CTTGCTCTTTAACTGGAAGTACAGCTCTGTCATTCCCTCCACACGACAAAG200.11434452003887714No Hit
GCTGCATTACATGTCTGTCAATATAAATTGACCATTCTTCTAGAGCATTCA190.10862729403693329No Hit
GCCAGAACCAGAAGGGCATTCCAAGGCTTCCCCACATTTCCTCCATTTACG190.10862729403693329No Hit
CTTTAGTACAAAATTAAAAATCTCCCTTGAAGTCTGGGGATCTTCTTCCAT190.10862729403693329No Hit
CTCGAGGCAGGAACACATCCAGGAAAATGACAGGTTTCATCTCTCTAAAGG190.10862729403693329No Hit
GCCATTTTAGTTTCGAGAGGGGAATCTTTGGCCCACCATGAATCACACTTC190.10862729403693329No Hit
GTCCAGGGTTCTCATCGATCAATCCTTGAATCAGCACATCTTTTGCCAACT190.10862729403693329No Hit
CGGCGCTTCAGGTCGATCAGATTTTCAAAAATAAGCAAGTTCTTTTCTGGT190.10862729403693329No Hit
CCCATTTGCTGCTTCTCTGGCTTCTTTCCTAATTTCAATGTACTTTTTCTT190.10862729403693329No Hit
CTAACAACAACCATGGGATTTTTGAAGTTATCCCAAATTCAATGCAAAGCA190.10862729403693329No Hit
CTCCTCCATTGTTTTCAGAAGCAGCCAGCTGCAGCAAGGGGCTAAGCCAGT190.10862729403693329No Hit
CTGCATTATTTATCATCTCACTAGGATGAACTTCACCCAATAATCCTAGGA190.10862729403693329No Hit
GTTTTTGCTAGTTCAGCTCTTACATCATTGCTCCAATCCTTAAAGAGCAGT190.10862729403693329No Hit
TGTAGATATTATTAAAGGCAAGTGATGCTCCCAGCCTCTTGAAAGCATTGG190.10862729403693329No Hit
GTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAAAA190.10862729403693329No Hit
CTCCTAGAGATCCAAGCATTTGTACTACTCTAATTCTTATTTCTTCTAAGG190.10862729403693329No Hit
CTTGGCATCTGATAAACACGGTCGTCACCAGTGTCTGTCTGGGTGAGGAAC190.10862729403693329No Hit
GGGGTGATGAGGCTGCTGTGCTTGATCTGAATGTCAGGAAGGTCTCCGTGC180.10291006803498942No Hit
GTCAGAAGATTTTTGTTTATTTGTCCTCCTAGAGATCCAAGCATTTGTACT180.10291006803498942No Hit
TGGGTCTTCAGGAGAGTGTTTCAGACTCTTTGGACTAACTCCCTCGAAATA180.10291006803498942No Hit
TTCAGAAGTTCTCGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAA180.10291006803498942No Hit
TGTCAATATAAATTGACCATTCTTCTAGAGCATTCAGGCCTACTTCTGCCA180.10291006803498942No Hit
CCCACATTTCCTCCATTTACGGAGACAGCTGTTTCACTAACTTGCAGCACT180.10291006803498942No Hit
ATTTTTTTTTTCAATGCAAGTTCTCCATAGAATTTACTAAATAATTCTCCA180.10291006803498942No Hit
CCGTTTCTTTCATTGGTAACATCAGATTGATAAACTGGCGAGTTAGCCGAA180.10291006803498942No Hit
CGGAGTTCTACTGGTTTTAACTTTGGCATCATCTTATAAATTATGTCCAAA180.10291006803498942No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)