PAL2NAL outputPAL2NAL output >AMPA_PSEA7 gaattccttgttaaaagcgtacgcccggaaaccctgaaaaccgccaccctggtgctcgcc gtaggcgaaggccgcaagctcggcgccagcgccaaggcggtcgacgacgccaccggcggc gccatcgcagtgctcaagcgtggcgacctggccggcaaggtcggccagaccctgctcctg cagctgcccaatctcaaggccgagcgcgtgctgctggtcggcgccggcaaggagcgcgaa ctgggcgaccgccagtaccgcaagctggcctccgccgtgctgtcgaccctcaagggcctg gccggcgccgatgccctggccctcggcgatctcgcggtgaaaggccgcgatgcccacgcg aaggcgcgtctgctggtggaaaccctggccgatggcctgtacgtgttcgatcgctacaag agccagaaagccgagccgctgaaactgaagaagctgaccctgctcgccgacaaggccgac agcgccgccgtcgagcaaggcagcaaggaagctcaggccatcgccaatggcatggccctg acccgcgatctcggcaaccttccgccgaacgtctgccatccgacgttcctcggcgagcag gccaagctggccaaggagttcaagggcctgaaggtcgaggtcgacgagaaactgcgcgag ctgggcatgggctcgttcctcgccgtcgcccagggcagccagccgccgcggctgatcgtc ctgcaatacaacggcgcgaagaaggaccaggccccgcacgtgctggtcggcaagggcatc accttcgacaccggcggcatcagcctcaagccgggcctgggcatggacgagatgaagttc gacatgtgcggcgccgccagcgtcttcggcaccttccgcgcggtgctcgaactgcagttg ccgatcaacctggtcggcctgctggcctgcgccgagaacatgcccagcggcggagccacc cgtccgggcgacatcgtcaccaccatgagcgggcagaccgtcgagatcctcaacaccgac gccgaagggcgcctggtgctgtgcgatgcactgacctacgccgagcgcttcaagcctcag tcggtggacatcgccaccctcaccggcgcctgcatcgtcgccctgggcagcaacacctcg ggccttatgggcaacaacgaagcgctggtcaggcaactgctcaaggccggcgagttcgcc gacgaccgcgcctggcaattgccgctgttcgacgaataccaggaacagctggacagcccg ttcgccgacatcgccaacatcggcgggccgaaggccggcaccatcaccgccggctgcttc ctctcgcgcttcgccaagaaataccactgggcgcacttggacatcgccggcaccgcctgg atcagcggcaaggacaaaggcgccaccggccgcccggttccgttgttgacccagtacctg ctggaacgggccaag >AMPA_ECOLI gagtttagtgtaaaaagcggtagcccggagaaacagcggagtgcctgcatcgtcgtgggc gtcttcgaaccacgtcgcctttctccgattgcagaacagctcgataaaatcagcgatggg tacatcgccctgctacgtcggggcgaactggaaggaaaaccggggcagacattgttgctg cacgttccgaatgtactttccgagcgaattctccttattggttgcggcaaagaacgtgag ctggatgagcgtcagtacaagcaggttattcagaaaaccattaatacgctgaatgatact ggctcaatggaagcgtgctttctgactgagctgcacgttaaaggccgtaacaactactgg aaagtgcgtcaggctgtcgagacggcaaaagagacgctctacagtttcgatcagctgaaa acgaacaagagcgaaccgcgtcgtctgcgtaagatggtgttcgtgccgacccgccgtgaa ctgaccagcggtgagcgcgcgatccagcacggtctggcgattgccgccgggattaaagca gcaaaagatctcggcaatatgccgccgaatatctgtaacgccgcttacctcgcttcacaa gcgcgcctggctgacagctacaagaatgtcatcacccgcgttggcgaacagatgaaagag ctggggatgcattcctatctggcggtcggtcagggttcgaacgaatcgctgatgtcggtg attgagtacaaaggcgcgtcggaagatgcacgcccaatcgtgctggtgggtaaaggttta accttcgactccggcggtatctcgatcaagccttcagaaggcatggatgagatgaagtac gatatgtgcggtgcggcagcggtttacggcgtgatgcggatggtcgcggagctacaactg ccgattaacgttatcggcgtgttggcaggctgcgaaaacatgcctggcggacgagcctat cgtccgggcgatgtgttaaccaccatgtccggtcaaaccgttgaagtgctgaacaccgac gctgaaggccgcctggtactgtgcgacgtgttaacttacgttgagcgttttgagccggaa gcggtggacgtggcgacgctgaccggtgcctgcgtgatcgcgctgggtcatcatattact ggtctgatggcgaaccataatccgctggcccatgaactgattgccgcgtctgaacaatcc ggtgaccgcgcatggcgcttaccgctgggtgacgagtatcaggaacaactggagtccaat tttgccgatatggcgaacattggcggtcgtcctggtggggcgattaccgcaggttgcttc ctgtcacgctttacccgtaagtacaactgggcgcacctggatatcgccggtaccgcctgg cgttctggtaaagcaaaaggcgccaccggtcgtccggtagcgttgctggcacagttcctg ttaaaccgcgctggg Synonymous (KS) and non-synonymous (KA) substitution rates calcualted by codeml in the PAML package: KS = 60.4277 KA = 0.3476 KA/KS = 0.0058 You can see the parameters used in codeml to calculate these values. If you use these values, please cite the following peper: Goldman, N. and Yang, Z. 1994. A codon-based model of nucleotide substitution for protein-coding DNA sequences. Molecular Biology and Evolution 11:725-736.