FastQCFastQC Report
Вт 31 окт 2017
sra_data.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamesra_data.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25828
Sequences flagged as poor quality0
Sequence length97-10000
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATTG150.0013995072248.934119
TGTTCTT1550.0228.858761
TATTCTG353.0527553E-7213.37211
GTAGGGG300.007985249209.88564480-489
CTTTGTA555.820766E-11203.673365
TTATTGA304.4413136E-5186.700598
TATCCTT200.0033107363186.700588
GTGTTTC1050.0177.810091
GTCTGTT1250.0164.296522
TCTTCAT358.210621E-5160.029074
TTTGTAT703.1468517E-10160.029076
GTCTTTG358.210621E-5160.029072
TCTTTGT857.2759576E-12153.753424
TGATCAT250.006453389149.360477
GCTCTTC250.006453389149.360479
TATTGAT250.006453389149.360479
AATTACT250.006453389149.360472
GAATTAC250.006453389149.360471
AGTTTCT755.0931703E-10149.360461
TGAGGAA700.007111766144.10994420-429
Produced by FastQC (version 0.11.2)