1: Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt 11):959-63.
Epub 2005 Oct 20.

The ybeY protein from Escherichia coli is a metalloprotein.

Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, 
Fiser A, Chance MR, Fedorov AA.

New York Structural Genomics Research Consortium (NYSGXRC), Albert Einstein
College of Medicine, Bronx, New York 10461, USA.

The three-dimensional crystallographic structure of the ybeY protein from
Escherichia coli (SwissProt entry P77385) is reported at 2.7 A resolution. YbeY
is a hypothetical protein that belongs to the UPF0054 family. The structure
reveals that the protein binds a metal ion in a tetrahedral geometry. Three
coordination sites are provided by histidine residues, while the fourth might be 
a water molecule that is not seen in the diffraction map because of its
relatively low resolution. X-ray fluorescence analysis of the purified protein
suggests that the metal is a nickel ion. The structure of ybeY and its sequence
similarity to a number of predicted metal-dependent hydrolases provides a
functional assignment for this protein family. The figures and tables of this
paper were prepared using semi-automated tools, termed the Autopublish server,
developed by the New York Structural GenomiX Research Consortium, with the goal
of facilitating the rapid publication of crystallographic structures that emanate
from worldwide Structural Genomics efforts, including the NIH-funded Protein
Structure Initiative.

PMCID: PMC1978141
PMID: 16511207 [PubMed - indexed for MEDLINE]

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