[0.000001] Reading roadmap file velv/Roadmaps [0.038991] 3650 roadmaps read [0.039083] Creating insertion markers [0.041625] Ordering insertion markers [0.050941] Counting preNodes [0.052585] 5125 preNodes counted, creating them now [0.078981] Adjusting marker info... [0.082369] Connecting preNodes [0.088398] Cleaning up memory [0.088557] Done creating preGraph [0.088562] Concatenation... [0.090527] Renumbering preNodes [0.090529] Initial preNode count 5125 [0.090652] Destroyed 4462 preNodes [0.090653] Concatenation over! [0.090655] Clipping short tips off preGraph [0.090745] Concatenation... [0.090953] Renumbering preNodes [0.090954] Initial preNode count 663 [0.090992] Destroyed 209 preNodes [0.090994] Concatenation over! [0.090995] 107 tips cut off [0.090996] 454 nodes left [0.091096] Writing into pregraph file velv/PreGraph... [0.094702] Reading read set file velv/Sequences; [0.095906] 3650 sequences found [0.100518] Done [0.103166] Reading pre-graph file velv/PreGraph [0.103192] Graph has 454 nodes and 3650 sequences [0.105344] Scanning pre-graph file velv/PreGraph for k-mers [0.106764] 18832 kmers found [0.109581] Sorting kmer occurence table ... [0.114927] Sorting done. [0.114929] Computing acceleration table... [0.173987] Computing offsets... [0.177654] Ghost Threading through reads 0 / 3650 [0.179405] === Ghost-Threaded in 0.005138 s [0.181017] Threading through reads 0 / 3650 [0.192445] === Threaded in 0.013035 s [0.199733] Correcting graph with cutoff 0.200000 [0.199925] Determining eligible starting points [0.200303] Done listing starting nodes [0.200305] Initializing todo lists [0.200362] Done with initilization [0.200364] Activating arc lookup table [0.200477] Done activating arc lookup table [0.203963] Concatenation... [0.203974] Renumbering nodes [0.203976] Initial node count 454 [0.203987] Removed 267 null nodes [0.203988] Concatenation over! [0.203989] Clipping short tips off graph, drastic [0.204028] Concatenation... [0.204047] Renumbering nodes [0.204047] Initial node count 187 [0.204051] Removed 25 null nodes [0.204052] Concatenation over! [0.204053] 162 nodes left [0.204167] Writing into graph file velv/Graph... [0.207405] WARNING: NO COVERAGE CUTOFF PROVIDED [0.207407] Velvet will probably leave behind many detectable errors [0.207408] See manual for instructions on how to set the coverage cutoff parameter [0.207411] Removing contigs with coverage < -1.000000... [0.207420] Concatenation... [0.207426] Renumbering nodes [0.207427] Initial node count 162 [0.207429] Removed 0 null nodes [0.207430] Concatenation over! [0.207435] Concatenation... [0.207441] Renumbering nodes [0.207442] Initial node count 162 [0.207443] Removed 0 null nodes [0.207444] Concatenation over! [0.207447] Clipping short tips off graph, drastic [0.207452] Concatenation... [0.207457] Renumbering nodes [0.207458] Initial node count 162 [0.207460] Removed 0 null nodes [0.207461] Concatenation over! [0.207462] 162 nodes left [0.207463] WARNING: NO EXPECTED COVERAGE PROVIDED [0.207464] Velvet will be unable to resolve any repeats [0.207465] See manual for instructions on how to set the expected coverage parameter [0.207466] Concatenation... [0.207472] Renumbering nodes [0.207473] Initial node count 162 [0.207475] Removed 0 null nodes [0.207476] Concatenation over! [0.207477] Removing reference contigs with coverage < -1.000000... [0.207485] Concatenation... [0.207491] Renumbering nodes [0.207492] Initial node count 162 [0.207493] Removed 0 null nodes [0.207494] Concatenation over! [0.207551] Writing contigs into velv/contigs.fa... [0.209920] Writing into stats file velv/stats.txt... [0.210513] Writing into graph file velv/LastGraph... Final graph has 162 nodes and n50 of 315, max 1333, total 15408, using 0/3650 reads