Nucleotide database
Last update on the 21st of October, 2017In this task we study several nucleotide databases of NCBI and capabilities they provide.
File | Link |
---|---|
Mitochondrion protein genes | protein_genes.ods |
Brief description of tasks
The following tasks were done and reported via Google Forms:
- quallity assession of Zea mays genome assembly;
- 7 keys used in feature tables were described;
- Global Ocean Sampling Expedition project description;
- mitochondrial genomes of Paramecium caudatum were found and counted.
Some tasks needed additional information, which is stored in this page.
Mitochondrial proteins of P. caudatum
protein_genes.ods
P. caudatum (fig. 1) is one of the most recognizable species in Ciliophora taxon. To investigate its mitochondrion genome a query
(paramecium caudatum[Organism]) AND gene_in_mitochondrion[PROP] and "complete genome"[KYWD]
was submitted
into NCBI databases. Two entries were found, a RefSeq database one under accession NC_014262
was taken for futher investigation.
The table with genomic organisation of mitochondrial proteins is available in protein_genes.ods
file.
Several species were studied for whole mitochondrial genomes. Search results and processed data is shown in table 1. Mitochondrion genome size and number of its genes are correlated so does MTgenome and genome size. Interestingly, genome size grows faster then MTgenome one, which stands for uncommon functions of mitochondria and nuclei in cell life.
Group | Organism | MTgenome size, bp | Genes | Genome size, Mb | MT/genome ratio, 10^-6 |
---|---|---|---|---|---|
Opisthokonta | Homo sapiens | 16569 | 37 | 2996 | 5,53 |
Chromalveolata | Paramecium caudatum | 43660 | 49 | 30 | 1455,33 |
Opisthokonta | Saccharomyces cerevisiae YJM1242 | 95658 | 46 | 12 | 7971,50 |
Excavata | Jakoba libera strain ATCC 50422 | 100252 | 115 | NA | NA |
Viridiplantae | Arabidopsis thaliana | 366924 | 131 | 116 | 3163,14 |
Genome sizes
To estimate typical genome sizes of life kingdoms the NCBI Genome database was used. Obtained data was proccessed through LibreOffice Calc and results were merged into table 2. Median size is proposed as typical for the corresponding group of organisms.
Group | Minial | Median | Maximal |
---|---|---|---|
Viroids | 246 | 348 | 434 |
Viruses | 220 | 10044 | 2473870 |
Archea | 137797 | 1580350 | 6451200 |
Bacteria | 104827 | 2752850 | 68003500 |
Eukaryota | 245805 | 53011000 | 27602700000 |