AatI	NEBC13653	Type II	R	AGGCCT	274	-	-	-	-	no	-

AbrI	X62690	Type II	R	CTCGAG	222	-	CAA44567.1	P26919	38661	no	-

ApaI	HQ327698	Type II	R	GGGCCC	355	-	ADO24182.1	E3VXA1	308229533	no	-

BstVI	L07643	Type II	R	CTCGAG	224	-	AAA22281.1	P43421	142619	no	-

BsuMIA	AL009126	Type II	R	CTCGAG	313	BSU_06090	CAB12428.1	O35025	-	no	BsuMI

BsuMIB	AL009126	Type II	R	CTCGAG	343	BSU_06100	CAB12429.1	O34885	-	no	BsuMI

BsuMIC	AL009126	Type II	R	CTCGAG	465	BSU_06110	CAB12430.1	O34303	-	no	BsuMI

CceI	CP001348	Type II	R	CCGG	399	Ccel_2866	ACL77160.1	B8I860	-	no	-

CviAII	JF411744	Type II	R	CATG	342	-	AAC97063.2	O41016	338221978	no	-

Eco1524I	EU863203	Type II	R	AGGCCT	272	-	-	-	-	no	-

FpsJI	AM398681	Type II	R	CCGG	357	FP2273	CAL44329.1	A6H1V5	149772845	no	-

FseI	JF323049	Type II	R	GGCCGGCC	219	-	ADX97304.1	F1KC51	323650452	no	-

Hpy299IX	CP005491	Type II	R	CCGG	229	K749_04345	AGL68244.1	-	499059329	no	-

HpyCH4I	AF459446	Type II	R	CATG	228	-	AAL59391.1	Q8VQ95	18092657	no	-

NflHII	ACEN01000062	Type II	R	CCGG	377	NEIFLAOT_01355	EEG33647.1	C0EN25	224952438	no	-

NgoBV	U43735	Type II	R	GGNNCC	175	-	AAA86269.1	Q50975	1165243	no	-

NgoMV	CP003909	Type II	R	GGNNCC	243	NGFG_01122	EEZ47955.1	-	268583279	no	-

NheI	AY462277	Type II	R	GCTAGC	328	-	AAR23810.1	Q6S994	38564763	no	-

NlaIV	U06074	Type II	R	GGNNCC	243	-	AAA53238.1	P50183	476228	no	-

PaeR7I	X03274	Type II	R	CTCGAG	246	-	CAA27026.1	P05104	45387	no	-

PciI	JX481785	Type II	R	ACATGT	257	-	AFV09321.1	K4LNB0	409034609	no	-

R1.BsuMI	CP010052	Type II	R	CTCGAG	313	QU35_03530	AIY91901.1	-	728883506	no	-

R2.BsuMI	CP010052	Type II	R	CTCGAG	343	QU35_03535	AIY91902.1	-	728883507	no	-

R3.BsuMI	CP010052	Type II	R	CTCGAG	465	QU35_03540	AIY91903.1	-	728883508	no	-

SmoLI	CP001779	Type II	R	CCGG	336	Smon_0101	ACZ00597.1	D1AWC1	268314223	no	-

SpeI	NEBC15359	Type II	R	ACTAGT	208	-	-	-	-	no	-

TliI	CP006670	Type II	R	CTCGAG	281	OCC_13281	EHR77447.1	H3ZRP1	374741014	no	-

XhoI	NEBC13645	Type II	R	CTCGAG	246	-	-	-	-	no	-