TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= KPYK1_YEAST P00549 Pyruvate kinase 1 (PK 1) (2.7.1.40 {ECO:0000269|PubMed:10413488}) (cell division cycle protein 19) Length=500 Score E Sequences producing significant alignments: (Bits) Value scaffold-361 507 1e-161 scaffold-115 506 3e-161 unplaced-1090 28.5 6.5 scaffold-444 28.9 7.3 scaffold-123 28.5 9.1 > scaffold-361 Length=455836 Score = 507 bits (1305), Expect = 1e-161, Method: Compositional matrix adjust. Identities = 266/492 (54%), Positives = 347/492 (71%), Gaps = 22/492 (4%) Frame = +3 Query 27 IGPKTNNPETLVALRKAGLNIVRMNFSHGSYEYHKSVIDNARKSEELYPGRPLAIALDTK 86 +GPKTN+ + + LR+AG+NIVRMNFSHGSYEYH SVI NAR S + GRP+AIALDTK Sbjct 218580 VGPKTNSVDMMCKLRQAGMNIVRMNFSHGSYEYHGSVIKNARDSFQ*MSGRPVAIALDTK 218759 Query 87 GPEIRTGTTTNDVDYPIPPNHEMIFTTDDKYAKACDDKIMYVDYKNITKVISAGRIIYVD 146 GPEIRTG D + IP HEM+ TTD A D +Y+DY+ + G+ I+VD Sbjct 218760 GPEIRTGRIKGD*ELSIPHGHEMVLTTDPARANDGDMDCIYIDYEKLPHSTDVGKYIFVD 218939 Query 147 DGVLSFQVLEVVDDKTLKVKALNAGKICSHKGVNLPGTDVDLPALSEKDKEDLRFGVKNG 206 DG L FQV+ + K ++V A N+ KI ++KGVNLP T V+LPALSE+DK DL+FG++N Sbjct 218940 DGQLRFQVVGI-QGKDVRV*AQNSWKISNNKGVNLPMTTVELPALSERDKRDLQFGIEND 219116 Query 207 VHMVFASFIRTANDVLTIREVLGE----QGKDVKIIVKIENQQGVNNFDEILKVTDGVMV 262 V M+FASFIR D+L IR++L +GKD+KII KIE+ +G++NFDEIL+VTDG+MV Sbjct 219117 VDMIFASFIRKKEDILEIRQILDNFMNGRGKDIKIIAKIESHEGLDNFDEILQVTDGIMV 219296 Query 263 ARGDLGIEIPAPEVLAVQKKLIAKSNLAGKPVICATQMLESMTYNPRPTRAEVSDVGNAI 322 ARGDLGIEI A +V QK +IAK N GKP+ICATQMLE+MTYNPRPTRAEVSDV NA+ Sbjct 219297 ARGDLGIEIAAEKVFIAQKMMIAKCNAVGKPIICATQMLETMTYNPRPTRAEVSDVANAV 219476 Query 323 LDGADCVMLSGETAKGNYPINAVTTMAETAVIAEQAIAYLPNYDDMRNCTPKPtsttetv 382 LDGADCVMLSGETAKG YP+ V M AE + Y ++++R+ TPKPT+TTE + Sbjct 219477 LDGADCVMLSGETAKGQYPLETVDMMNRLCKEAESVLFYPQLFNELRDVTPKPTTTTEAI 219656 Query 383 aasavaavFEQKAKAIIVLSTSGTTPRLVSKYRPNCPIILVTRCPRAARFSHLYRGVFPF 442 A+SAV A E A AI VL+T+G + RL+SKYRP+ P+I VTR + AR HLYRG +PF Sbjct 219657 ASSAVNAAEE*GAAAICVLTTTGESARLISKYRPHIPVITVTRNAKVARQCHLYRGCYPF 219836 Query 443 VFEK--------EPVS---------DWTDDVEARINFGIEKAKEFGILKKGDTYVSIQGF 485 V+EK P S W +DV+ R + +++ K+ G+LK GD V++QG+ Sbjct 219837 VYEKARKQGSESAPASPNS*SMVHDQWQEDVDERFFWAMKEGKKLGMLKAGDVIVAVQGW 220016 Query 486 KAGAGHSNTLQV 497 + GAG ++ +++ Sbjct 220017 RGGAGSTSVMRM 220052 > scaffold-115 Length=160031 Score = 506 bits (1303), Expect = 3e-161, Method: Compositional matrix adjust. Identities = 266/492 (54%), Positives = 347/492 (71%), Gaps = 22/492 (4%) Frame = -1 Query 27 IGPKTNNPETLVALRKAGLNIVRMNFSHGSYEYHKSVIDNARKSEELYPGRPLAIALDTK 86 +GPKTN+ + + LR+AG+NIVRMNFSHGSYEYH SVI NAR S + GRP+AIALDTK Sbjct 32951 VGPKTNSVDMMCKLRQAGMNIVRMNFSHGSYEYHGSVIKNARDSFQ*MSGRPVAIALDTK 32772 Query 87 GPEIRTGTTTNDVDYPIPPNHEMIFTTDDKYAKACDDKIMYVDYKNITKVISAGRIIYVD 146 GPEIRTG D + IP HEM+ TTD A D +Y+DY+ + G+ I+VD Sbjct 32771 GPEIRTGRIKGD*ELSIPHGHEMVLTTDPARANDGDIDCIYIDYEKLPHSTDVGKYIFVD 32592 Query 147 DGVLSFQVLEVVDDKTLKVKALNAGKICSHKGVNLPGTDVDLPALSEKDKEDLRFGVKNG 206 DG L FQV+ + + ++V A N+ KI ++KGVNLP T V+LPALSE+DK DL+FG++N Sbjct 32591 DGQLRFQVVSI-QGREVRV*AQNSWKISNNKGVNLPMTTVELPALSERDKRDLQFGIEND 32415 Query 207 VHMVFASFIRTANDVLTIREVLGE----QGKDVKIIVKIENQQGVNNFDEILKVTDGVMV 262 V M+FASFIR D+L IR++L +GKD+KII KIE+ +G++NFDEIL+VTDG+MV Sbjct 32414 VDMIFASFIRKKEDILEIRQILDTFMNGRGKDIKIIAKIESHEGLDNFDEILQVTDGIMV 32235 Query 263 ARGDLGIEIPAPEVLAVQKKLIAKSNLAGKPVICATQMLESMTYNPRPTRAEVSDVGNAI 322 ARGDLGIEI A +V QK +IAK N GKP+ICATQMLE+MTYNPRPTRAEVSDV NA+ Sbjct 32234 ARGDLGIEIAAEKVFIAQKMMIAKCNAVGKPIICATQMLETMTYNPRPTRAEVSDVANAV 32055 Query 323 LDGADCVMLSGETAKGNYPINAVTTMAETAVIAEQAIAYLPNYDDMRNCTPKPtsttetv 382 LDGADCVMLSGETAKG YP+ V M AE + Y ++++R+ TPKPTSTTE + Sbjct 32054 LDGADCVMLSGETAKGQYPLETVDMMNRLCKEAESVLFYPQLFNELRDVTPKPTSTTEAI 31875 Query 383 aasavaavFEQKAKAIIVLSTSGTTPRLVSKYRPNCPIILVTRCPRAARFSHLYRGVFPF 442 A+SAV A E A AI VL+T+G + RL+SKYRP+ P+I VTR + AR HLYRG +PF Sbjct 31874 ASSAVNAAEE*GAAAICVLTTTGESARLISKYRPHIPVITVTRNAKVAR*CHLYRGCYPF 31695 Query 443 VFEK--------EPVS---------DWTDDVEARINFGIEKAKEFGILKKGDTYVSIQGF 485 V+EK P S W +DV+ R + +++ K+ G+LK GD V++QG+ Sbjct 31694 VYEKARKQGSESAPASPNS*SMVHDQWQEDVDERFFWAMKEGKKLGMLKAGDVIVAVQGW 31515 Query 486 KAGAGHSNTLQV 497 + GAG ++ +++ Sbjct 31514 RGGAGSTSVMRM 31479 > unplaced-1090 Length=909 Score = 28.5 bits (62), Expect = 6.5, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 17/30 (57%), Gaps = 2/30 (7%) Frame = +1 Query 415 RPNCPIILVTRCPRAARFSHLYRGVFPFVF 444 R NCP + TRC R A + L +FPF + Sbjct 721 RANCPTLWATRCSRLA--TTLTSTIFPFKY 804 > scaffold-444 Length=851481 Score = 28.9 bits (63), Expect = 7.3, Method: Compositional matrix adjust. Identities = 17/52 (33%), Positives = 28/52 (54%), Gaps = 1/52 (2%) Frame = +2 Query 39 ALRKAGLNIVRMNFSHGSY-EYHKSVIDNARKSEELYPGRPLAIALDTKGPE 89 AL +AG + + H + + K +++ R SEE YPGR A+ +D + E Sbjct 780023 ALSEAGSAQIILAMFHAEWADESKLMMEVLRVSEEKYPGRVKALVIDAEEAE 780178 > scaffold-123 Length=442268 Score = 28.5 bits (62), Expect = 9.1, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 17/30 (57%), Gaps = 2/30 (7%) Frame = -2 Query 415 RPNCPIILVTRCPRAARFSHLYRGVFPFVF 444 R NCP + TRC R A + L +FPF + Sbjct 349963 RANCPTLWATRCSRLA--TTLTSTIFPFKY 349880 Score = 28.5 bits (62), Expect = 9.1, Method: Compositional matrix adjust. Identities = 13/30 (43%), Positives = 17/30 (57%), Gaps = 2/30 (7%) Frame = +3 Query 415 RPNCPIILVTRCPRAARFSHLYRGVFPFVF 444 R NCP + TRC R A + L +FPF + Sbjct 366447 RANCPTLWATRCSRLA--TTLTSTIFPFKY 366530 Lambda K H a alpha 0.317 0.135 0.387 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3103144310 Database: X5.fasta Posted date: Nov 24, 2021 5:07 AM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40