TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= FUMH_YEAST P08417 Fumarate hydratase, mitochondrial (Fumarase {ECO:0000303|PubMed:3040736}) (4.2.1.2 {ECO:0000269|PubMed:11585823, ECO:0000269|PubMed:20231875, ECO:0000269|PubMed:3040736}) (Precursor) Length=488 Score E Sequences producing significant alignments: (Bits) Value scaffold-423 630 0.0 scaffold-162 29.3 5.1 > scaffold-423 Length=1446679 Score = 630 bits (1625), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/475 (66%), Positives = 374/475 (79%), Gaps = 9/475 (2%) Frame = -1 Query 22 RRM-------NSSFRTETDAFGEIHVPADKYWGAQTQRSFQNFKIGGARERMPLPLVHAF 74 RRM N FR E D FG+I+VPA+ YWGAQTQRS QNF I +RMPLPL+HAF Sbjct 304171 RRMSAIVGAGNGKFRVEKDTFGDINVPAEAYWGAQTQRSIQNFAINQENDRMPLPLIHAF 303992 Query 75 GVLKKSAAIVNESLGGLDPKISKAIQQAADEVASGKLDDHFPLVVFQTGSGTQSNMNANE 134 ++KK+ AIVN G L ++ I +AADE+ SGKLD+HFPLVV+ TGSGTQ+NMN NE Sbjct 303991 AMVKKACAIVNCD*GTLPAQLRDGIVKAADEIISGKLDNHFPLVVW*TGSGTQTNMNVNE 303812 Query 135 VISNRAIEILGGKIGSKQ-VHPNNHCNQSQSSNDTFPTVMHIAASLQIQNELIPELTNLK 193 VISNRAIEILGG +GSK VHPN+H N+ QSSNDTFPT MH+AA L+++ ++P LT L+ Sbjct 303811 VISNRAIEILGG*MGSKSPVHPNDHVNKGQSSNDTFPTAMHVAAVLEVEKRVLPALTRLR 303632 Query 194 NALEAKSKEFDHIVKIGRTHLQDATPLTLGQEFSGYVQQVENGIQRVAHSLKTLSFLAQG 253 +AL++ + H++KIGRTHLQDATPLTL EFSGYV Q++N I R+ +L L LAQG Sbjct 303631 HALDSFATRHSHVIKIGRTHLQDATPLTLE*EFSGYVTQLDNSIHRIKSTLPHLRQLAQG 303452 Query 254 GTAVGTGLNTKPGFDVKIAEQISKETGLKFQTAPNKFEALAAHDAIVECSGALNTLACSL 313 GTAVGTGLNTK GF IA +S TG KF+TAPNKFEALAAHDA+V GALNT A SL Sbjct 303451 GTAVGTGLNTKSGFAEAIA**LSTLTGTKFETAPNKFEALAAHDALV*GHGALNTTAVSL 303272 Query 314 FKIAQDIRYLGSGPRCGYHELMLPENEPGSSIMPGKVNPTQNEALTQVCVQVMGNNAAIT 373 KIA DIR+LGSGPRCG EL LPENEPGSSIMPGKVNPTQNEA+T VC QVMGNN A+T Sbjct 303271 MKIANDIRFLGSGPRCGLGELQLPENEPGSSIMPGKVNPTQNEAMTMVCAQVMGNNTAVT 303092 Query 374 FAGSQGQFELNVFKPVMIANLLNSIRLITDAAYSFRVHCVEGIKANEPRIHELLTKSLML 433 FAGSQG FELNVFKPV+I N L+S RLI DA+ SFR +C++G+KANE RI +L+ +SLML Sbjct 303091 FAGSQGHFELNVFKPVIIKNFLHSCRLIADASDSFRKNCIDGLKANEKRIGQLMQESLML 302912 Query 434 VTALNPKIGYDAASKVAKNAHKKGITLKESAL-ELGVLTEKEFDEWVVPEHMLGP 487 VTALNP IGYD A+ VAK AHK+G+TL+ESA+ +L VLT++EFD++V PE MLGP Sbjct 302911 VTALNPYIGYDKAALVAKTAHKEGLTLRESAVNKLKVLTDEEFDKYVKPEQMLGP 302747 Score = 627 bits (1617), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/475 (66%), Positives = 372/475 (78%), Gaps = 9/475 (2%) Frame = +1 Query 22 RRM-------NSSFRTETDAFGEIHVPADKYWGAQTQRSFQNFKIGGARERMPLPLVHAF 74 RRM N FR E D FG+I+VP D YWGAQTQRS QNF I +RMPLPL+HAF Sbjct 995983 RRMSSIVGAGNGKFRVEKDTFGDINVPDDAYWGAQTQRSIQNFAINQESDRMPLPLIHAF 996162 Query 75 GVLKKSAAIVNESLGGLDPKISKAIQQAADEVASGKLDDHFPLVVFQTGSGTQSNMNANE 134 ++KK+ AIVN G L + I +AADE+ SGKLDDHFPLVV+ TGSGTQ+NMN NE Sbjct 996163 AMVKKACAIVNCD*GTLPA*LRDGIVKAADEIISGKLDDHFPLVVW*TGSGTQTNMNVNE 996342 Query 135 VISNRAIEILGGKIGSKQ-VHPNNHCNQSQSSNDTFPTVMHIAASLQIQNELIPELTNLK 193 VISNRAIEILGG++GSK VHPN+H N+ SSNDTFPT MH+AA L+++ ++P LT L+ Sbjct 996343 VISNRAIEILGGQMGSKSPVHPNDHVNKG*SSNDTFPTAMHVAAVLEVEKRVLPALTRLR 996522 Query 194 NALEAKSKEFDHIVKIGRTHLQDATPLTLGQEFSGYVQQVENGIQRVAHSLKTLSFLAQG 253 +AL + + H++KIGRTHLQDATPLTL QEFSGYV Q+EN I R+ +L L LAQG Sbjct 996523 DALGSFATRHSHVIKIGRTHLQDATPLTLEQEFSGYVTQLENSIHRI*STLPHLRQLAQG 996702 Query 254 GTAVGTGLNTKPGFDVKIAEQISKETGLKFQTAPNKFEALAAHDAIVECSGALNTLACSL 313 GTAVGTGLNTK GF IA+ +S TG KF+TAPNKFEALAAHDA+V GALNT A SL Sbjct 996703 GTAVGTGLNTKSGFAEAIAQ*LSTLTGTKFETAPNKFEALAAHDALV*GHGALNTTAVSL 996882 Query 314 FKIAQDIRYLGSGPRCGYHELMLPENEPGSSIMPGKVNPTQNEALTQVCVQVMGNNAAIT 373 KIA DIR+LGSGPRCG EL LPENEPGSSIMPGKVNPTQNEA+T +C QVMGNN A+T Sbjct 996883 MKIANDIRFLGSGPRCGLGELQLPENEPGSSIMPGKVNPTQNEAMTMICAQVMGNNTAVT 997062 Query 374 FAGSQGQFELNVFKPVMIANLLNSIRLITDAAYSFRVHCVEGIKANEPRIHELLTKSLML 433 FAGS G FELNVFKPV+I N L+S RLI DA+ SFR +C++G+KANE RI +L+ +SLML Sbjct 997063 FAGS*GHFELNVFKPVIIKNFLHSCRLIADASDSFRKNCIDGLKANEKRIGQLM*ESLML 997242 Query 434 VTALNPKIGYDAASKVAKNAHKKGITLKESAL-ELGVLTEKEFDEWVVPEHMLGP 487 VTALNP IGYD A+ VAK AHK+G+TL+ESA+ +L VLT++EFD++V PE MLGP Sbjct 997243 VTALNPYIGYDKAALVAKTAHKEGLTLRESAVNKLKVLTDEEFDKYVKPEQMLGP 997407 > scaffold-162 Length=314529 Score = 29.3 bits (64), Expect = 5.1, Method: Compositional matrix adjust. Identities = 41/178 (23%), Positives = 75/178 (42%), Gaps = 12/178 (7%) Frame = -3 Query 185 LIPELTNLKNALEAKSKEFDHIVKIGRTHLQDATPLTLGQEFSGYVQQVENGIQRVAHSL 244 L+P+L + L + ++ + +G TH Q A T+G+ ++Q+ +Q + Sbjct 311194 LLPKLARCVDRLA*FA*KYADLPTLGFTHFQPAQLTTVGKRACLWLQEFIWDLQAL*RVR 311015 Query 245 KTLSFLAQGGTAVGTGLNTKPGFDV------KIAEQISKETGLKFQTAPNKFEALAAHDA 298 L F GT GT + F+ ++ + ++K G FQ + + Sbjct 311014 DELGFRGVKGT-TGTQASFLTLFNGDHDKV*QLDKLVTKYAG--FQKSYPVTG*TYSRKV 310844 Query 299 IVECSGALNTLACSLFKIAQDIRYLGSGPRCGYHELMLPENEPGSSIMPGKVNPTQNE 356 ++ L++ S K A DIR L + E +++ GSS M K NP ++E Sbjct 310843 DLDVLNVLSSFGASAHKFATDIRLLANLKEI---EEPFEKDQIGSSAMAYKRNPMRSE 310679 Lambda K H a alpha 0.316 0.132 0.378 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 3009582170 Database: X5.fasta Posted date: Nov 24, 2021 5:07 AM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40