MAST - Motif Alignment and Search Tool
MAST version 4.1.1 (Release date: Tue Apr 21 15:00:06 PDT 2009)
For further information on how to interpret these results or to get
a copy of the MAST software please access http://meme.nbcr.net.
REFERENCE
If you use this program in your research, please cite:
Timothy L. Bailey and Michael Gribskov,
"Combining evidence using p-values: application to sequence homology
searches", Bioinformatics, 14(48-54), 1998.
DATABASE AND MOTIFS
DATABASE ./5.fasta (peptide)
Last updated on Wed Jun 2 05:31:19 2010
Database contains 12 sequences, 4235 residues
MOTIFS ./memeout.txt (peptide)
MOTIF WIDTH BEST POSSIBLE MATCH
----- ----- -------------------
1 50 ANALQIHINPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGM
2 34 INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY
3 34 SKETVKWLYDIGVQYVDVSGKGGTNFAQIENDRR
PAIRWISE MOTIF CORRELATIONS:
MOTIF 1 2
----- ----- -----
2 0.15
3 0.14 0.17
No overly similar pairs (correlation > 0.60) found.
Random model letter frequencies (from non-redundant database):
A 0.073 C 0.018 D 0.052 E 0.062 F 0.040 G 0.069 H 0.022 I 0.056 K 0.058
L 0.092 M 0.023 N 0.046 P 0.051 Q 0.041 R 0.052 S 0.074 T 0.059 V 0.064
W 0.013 Y 0.033
SECTION I: HIGH-SCORING SEQUENCES
- Each of the following 12 sequences has E-value less than 10.
- The E-value of a sequence is the expected number of sequences
in a random database of the same size that would match the motifs as
well as the sequence does and is equal to the combined p-value of the
sequence times the number of sequences in the database.
- The combined p-value of a sequence measures the strength of the
match of the sequence to all the motifs and is calculated by
- finding the score of the single best match of each motif
to the sequence (best matches may overlap),
- calculating the sequence p-value of each score,
- forming the product of the p-values,
- taking the p-value of the product.
- The sequence p-value of a score is defined as the
probability of a random sequence of the same length containing
some match with as good or better a score.
- The score for the match of a position in a sequence to a motif
is computed by by summing the appropriate entry from each column of
the position-dependent scoring matrix that represents the motif.
- Sequences shorter than one or more of the motifs are skipped.
- The table is sorted by increasing E-value.
| Links | Sequence Name | Description | E-value | Length
|
|---|
|
| IDI2_LISMC
|
| 5.4e-112
| 358
|
|
| IDI2_LISMO
|
| 4.6e-111
| 358
|
|
| IDI2_LISW6
|
| 8.7e-110
| 358
|
|
| IDI2_STAHJ
|
| 1.2e-105
| 349
|
|
| IDI2_STAS1
|
| 3.1e-105
| 347
|
|
| IDI2_STAAS
|
| 5.7e-105
| 349
|
|
| IDI2_ENTFA
|
| 5.5e-97
| 347
|
|
| IDI2_BACCN
|
| 7e-97
| 349
|
|
| IDI2_BACSU
|
| 4.2e-96
| 349
|
|
| IDI2_NOCFA
|
| 8.7e-96
| 362
|
|
| IDI2_STRC1
|
| 1.6e-93
| 363
|
|
| IDI2_MOOTA
|
| 3.5e-91
| 346
|
SECTION II: MOTIF DIAGRAMS
- The ordering and spacing of all non-overlapping motif occurrences
are shown for each high-scoring sequence listed in Section I.
- A motif occurrence is defined as a position in the sequence whose
match to the motif has POSITION p-value less than 0.0001.
- The POSITION p-value of a match is the probability of
a single random subsequence of the length of the motif
scoring at least as well as the observed match.
- For each sequence, all motif occurrences are shown unless there
are overlaps. In that case, a motif occurrence is shown only if its
p-value is less than the product of the p-values of the other
(lower-numbered) motif occurrences that it overlaps.
- The table also shows the E-value of each sequence.
- Spacers and motif occurences are indicated by
- Spacers and motif occurences are indicated by
- Spacers and motif occurences are indicated by
- occurrence of motif `n' with p-value less than 0.0001.
- Sequences longer than 1000 are not shown to scale and are indicated by thicker lines.
| Links | Name | Expect | Motifs
|
|---|
|
| IDI2_LISMC
| 5.4e-112
|
|
|
| IDI2_LISMO
| 4.6e-111
|
|
|
| IDI2_LISW6
| 8.7e-110
|
|
|
| IDI2_STAHJ
| 1.2e-105
|
|
|
| IDI2_STAS1
| 3.1e-105
|
|
|
| IDI2_STAAS
| 5.7e-105
|
|
|
| IDI2_ENTFA
| 5.5e-97
|
|
|
| IDI2_BACCN
| 7e-97
|
|
|
| IDI2_BACSU
| 4.2e-96
|
|
|
| IDI2_NOCFA
| 8.7e-96
|
|
|
| IDI2_STRC1
| 1.6e-93
|
|
|
| IDI2_MOOTA
| 3.5e-91
|
|
| SCALE
|
| | |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| |
| 1 |
25 |
50 |
75 |
100 |
125 |
150 |
175 |
200 |
225 |
250 |
275 |
300 |
325 |
350 |
|
|---|
SECTION III: ANNOTATED SEQUENCES
- The positions and p-values of the non-overlapping motif occurrences
are shown above the actual sequence for each of the high-scoring
sequences from Section I.
- A motif occurrence is defined as a position in the sequence whose
match to the motif has POSITION p-value less than 0.0001 as
defined in Section II.
- For each sequence, the first line specifies the name of the sequence.
- The second (and possibly more) lines give a description of the
sequence.
- Following the description line(s) is a line giving the length,
combined p-value, and E-value of the sequence as defined in Section I.
- The next line reproduces the motif diagram from Section II.
- The entire sequence is printed on the following lines.
- Motif occurrences are indicated directly above their positions in the
sequence on lines showing
- the motif number of the occurrence,
- the position p-value of the occurrence,
- the best possible match to the motif, and
- columns whose match to the motif has a positive score (indicated
by a plus sign).
IDI2_LISMC
LENGTH = 358 COMBINED P-VALUE = 4.51e-113 E-VALUE = 5.4e-112
DIAGRAM: 79-[2]-33-[1]-[3]-128
[2] [1]
9.7e-34 1.2e
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANAL
++++++++++++++++++++++++++++++++++ ++++
76 HTKKINAELAEIAREAAIPMAVGSQSAALKNSSLIDTYKIVREINPNGMILANISPEVALQEGLRAIEMLEANAL
[3]
-56 3.3e-36
QIHINPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQI
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
151 QIHINPAQELVMQEGDRSFSHWLTRIEEYVKLSPVPVVVKEVGFGMTRETVKTLADIGVQTVDLAGKGGTNFAQI
ENDRR
+++++
226 ENDRRRDQAYDFLLDWGISTGQALIDMQHQDAPKIAYLASGGIRNPLDIVKALALGADSVGMAGQIIYSLKKEGV
IDI2_LISMO
LENGTH = 358 COMBINED P-VALUE = 3.87e-112 E-VALUE = 4.6e-111
DIAGRAM: 79-[2]-33-[1]-[3]-128
[2] [1]
6.1e-34 1.7e
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANAL
++++++++++++++++++++++++++++++++++ ++++
76 HTKKINAELAEIAREVAIPMAVGSQSAALKNSSLIDTYNIVREINPNGMILANVSPEVAIQDGLQAIEMLEANAL
[3]
-55 3.3e-36
QIHINPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQI
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
151 QIHINPAQELVMQEGDRSFSHWLTRIEEYVKLSPVPIVVKEVGFGMTRETVKTLADIGVQTVDLAGKGGTNFAQI
ENDRR
+++++
226 ENDRRRDQAYDFLLDWGISTGQALIDMQHQDAPKIAYLASGGIRNPLDIIKALALGADSVGMAGQIIYSLKKEGV
IDI2_LISW6
LENGTH = 358 COMBINED P-VALUE = 7.25e-111 E-VALUE = 8.7e-110
DIAGRAM: 79-[2]-33-[1]-[3]-128
[2] [1]
2.7e-34 2.2e
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANAL
++++++++++++++++++++++++++++++++++ ++++
76 HTKKINADLAEIAREVAIPMAVGSQSAALKNSSLMDTYQIVREVNPSGIIMANVSPEVAVQDGLRAIEMLEANAL
[3]
-56 1.1e-33
QIHINPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQI
+++++++++++++++++++++++ +++++++++++++++++++++++++++++++++++++++++++++++++++
151 QIHINPAQELVMQEGDRSFSHWLARIEEYVKRSPVPIIVKEVGFGMTRETVKTLREVGVETVDLAGKGGTNFAQI
ENDRR
+++++
226 ENDRRRDQAYDFLLDWGISTGQSLIDMQHIDAPKIAYLASGGIRNPLDIVKSLALGADSVGMAGQIIYSLKKDGV
IDI2_STAHJ
LENGTH = 349 COMBINED P-VALUE = 1.00e-106 E-VALUE = 1.2e-105
DIAGRAM: 75-[2]-33-[1]-[3]-123
[2] [1]
2.7e-34 3.1e-52
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHI
++++++++++++++++++++++++++++++++++ ++++++++
76 INEKLAVVARETGLAMAVGSTHAALRNPKMAESFSIARQTNPEGIIFSNVGADVPVDKAVEAVSLLDAQALQIHV
[3]
1.2e-33
NPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDR
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
151 NAPQELVMPEGNREFSTWLDNVAAIVQRVDVPVIIKEVGFGMSKELYKDLIDVGVTYVDVSGKGGTNFVTIENER
R
+
226 RSNKDMDYLANWGQSTVESLLESSAYQDSLNVFASGGVRTPLDVVKSLALGAKAVGMSRPFLNQVENGGITTTIE
IDI2_STAS1
LENGTH = 347 COMBINED P-VALUE = 2.60e-106 E-VALUE = 3.1e-105
DIAGRAM: 75-[2]-33-[1]-[3]-121
[2] [1]
1.8e-33 1.1e-51
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHI
++++++++++++++++++++++++++++++++++ ++++++++
76 INEKLAVIARETGIAMAVGSTHAALRNSKMASSFSIVRDTNPNGIIFSNVGADVPVDKAVESVKLLDAQALQVHV
[3]
1.4e-34
NPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDR
+++++++++++++++++++++ +++++++++++++++++++++++++++++++++++++++++++++++++++++
151 NAPQELVMPEGNRTFSTWMENLAQIVSRVDVPVIVKEVGFGMSKETIKSLNEIGVRYVDVSGRGGTNFVDIENER
R
+
226 RTYKDMDYLGLWGQTTVESLLESASYQQDMDILASGGVRTPLDAVKCLALGASAVGMSRPFLNQVENYGITETLN
IDI2_STAAS
LENGTH = 349 COMBINED P-VALUE = 4.71e-106 E-VALUE = 5.7e-105
DIAGRAM: 75-[2]-33-[1]-[3]-123
[2] [1]
3.2e-34 1.1e-51
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHI
++++++++++++++++++++++++++++++++++ ++++++++
76 INEKLAVVARETGLAMAVGSTHAALRNPRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHV
[3]
1.4e-33
NPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDR
+++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++
151 NSPQELVMPEGNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNFVDIENER
R
+
226 RANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALGAKATGMSRPFLNQVENNGIAHTVA
IDI2_ENTFA
LENGTH = 347 COMBINED P-VALUE = 4.58e-98 E-VALUE = 5.5e-97
DIAGRAM: 69-[2]-33-[1]-[3]-127
[2]
1.4e-2
INEKLA
++++++
1 MNRKDEHLSLAKAFHKEKSNDFDRVRFVHQSFAESAVNEVDISTSFLSFQLPQPFYVNAMTGGSQRAKEINQQLG
[1]
8 7.0e-53
YIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHINPPQEL
++++++++ ++++++++++++ ++++++ ++++++++++++++
76 IIAKETGLLVATGSVSAALKDASLADTYQIMRKENPDGLIFANIGAGLGVEEAKRALDLFQANALQIHVNVPQEL
[3]
5.6e-30
VMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDRR
++++++++++++++++++++++++++++++++++++++++ ++++++++++++++++++++++++++ ++
151 VMPEGDRDFTNWLTKIEAIVQAVEVPVIVKEVGFGMSQETLEKLTSIGVQAADVSGQGGTSFTQIENARRKKREL
IDI2_BACCN
LENGTH = 349 COMBINED P-VALUE = 5.87e-98 E-VALUE = 7e-97
DIAGRAM: 73-[2]-33-[1]-[3]-125
[2
2.
IN
++
1 MAREKRKLEHIEYALSTGQSRTHGFCDIEFVHKSLPNSSFESVTCETKIGELSLSSPIFINAMTGGGGERTLHIN
] [1]
8e-30 4.6e-50
EKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHINP
++++++++++++++++++++++++++++++++ ++++++++++
76 EQLAYVAKHHHLAMAVGSQMAALKEKREVDSYRIVRRVNPNGIVFANLGSEATVEQAKCAVDMIEANALQIHLNV
[3]
5.4e-31
PQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDRR
++++++++++++++++++++++++ +++++++++++++++++++++++++++++++++++++++++++++++++
151 IQELTMPEGDRDFKGVLKRIENIVLTSEVPVIVKEVGFGMSKETVQQLANIGVTAIDIGGQGGTNFAAVENERRN
IDI2_BACSU
LENGTH = 349 COMBINED P-VALUE = 3.51e-97 E-VALUE = 4.2e-96
DIAGRAM: 73-[2]-33-[1]-[3]-125
[2
1.
IN
++
1 MTRAERKRQHINHALSIGQKRETGLDDITFVHVSLPDLALEQVDISTKIGELSSSSPIFINAMTGGGGKLTYEIN
] [1]
0e-28 5.4e-53
EKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHINP
+++++++++++++++++++++++++++++ ++ ++++++++++
76 KSLARAASQAGIPLAVGSQMSALKDPSERLSYEIVRKENPNGLIFANLGSEATAAQAKEAVEMIGANALQIHLNV
[3]
7.7e-29
PQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDRR
+++++++++++++++ ++++++++++++++++++++++++++ +++++++++ ++++++++++++++++++ ++
151 IQEIVMPEGDRSFSGALKRIEQICSRVSVPVIVKEVGFGMSKASAGKLYEAGAAAVDIGGYGGTNFSKIENLRRQ
IDI2_NOCFA
LENGTH = 362 COMBINED P-VALUE = 7.25e-97 E-VALUE = 8.7e-96
DIAGRAM: 73-[2]-33-[1]-[3]-138
[2
5.
IN
++
1 MSSNRKDDHVRHAVDQHRDRTPVNDFDAIGFQHHALAGIDAADVDLGVDIAGKRWHTPLFINAMTGGSAAATDIN
] [1]
5e-29 2.3e-49
EKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHINP
++++++++++++++++++ +++++++++++++ ++++++++++
76 RGLAIAARETGLPVASGSLSAYFRDPGLAGSFRVLREENPHGVVIANVNATATLDQARRAVDLLAADALQIHLNA
[3]
6.3e-32
PQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDRR
++++++++++++++++++++++ ++++++++++++++++++++++ ++++++++++++++++++++++++++++
151 VQEIVMPEGDRSFRSWPRRIEHLAAGVPVPVIVKEVGFGLSRPTVAWLRDAGVAVADVGGRGGTNFARIENDRRP
IDI2_STRC1
LENGTH = 363 COMBINED P-VALUE = 1.34e-94 E-VALUE = 1.6e-93
DIAGRAM: 74-[2]-33-[1]-[3]-138
[
2
I
+
1 MTSAQRKDDHVRLAIEQHNAHSGRNQFDDVSFVHHALAGIDRPDVSLATSFAGISWQVPIYINAMTGGSEKTGLI
2] [1]
.6e-30 2.3e-47
NEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQIHIN
+++++++++++++++++++++++++++++++++ +++++++++
76 NRDLATAARETGVPIASGSMNAYIKDPSCADTFRVLRDENPNGFVIANINATTTVDNAQRAIDLIEANALQIHIN
[3]
2.6e-30
PPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIENDRR
++++++++++++++ ++++++++++++++++++++++++++++++++ +++ +++++++++++++++++++++++
151 TAQETPMPEGDRSFASWVPQIEKIAAAVDIPVIVKEVGNGLSRQTILLLADLGVQAADVSGRGGTDFARIENGRR
IDI2_MOOTA
LENGTH = 346 COMBINED P-VALUE = 2.95e-92 E-VALUE = 3.5e-91
DIAGRAM: 77-[2]-33-[1]-[3]-118
[2] [1]
2.2e-28 2.1e-4
INEKLAYIARETGIPMAVGSQHAYLKNPSCADTY ANALQI
+++ ++++++++++++++++++++++++++++++ ++++++
76 LAINAALARVARRTGIALAVGSQRAGLENKEWRESFTIVRRENANGLILANIGAGNSPADAGEAVAMIAADGLQV
[3]
6 8.7e-31
HINPPQELVMPEGDRSFSHWLTRIEHYCKRVPVPVIVKEVGFGMSKETVKWLYDIGVQYVDVSGKGGTNFAQIEN
+++++++++++++++ ++++++++++++++ +++++ ++++++++++++++++++++++++++++++++++++++
151 HLNAAQELIMPEGDRAFRGWLENIRGMVNTLGVPVIAKEVGFGLSRETALQLYQAGVRIMDVGGRGGTNFAAIEE
DRR
+++
226 RRRGRSVAALAGWGLSTAVSILEIRELGLPVEVVATGGIRSALDAARALALGAKIVGAAGYFLKILLEQGEDALT
Debugging Information
CPU: kodomo-count
Time 0.092005 secs.
mast ./memeout.txt -d ./5.fasta -ev 10.000000 -mt 0.000100
Button Help
Links to Entrez database at NCBI
Links to sequence scores (section I)
Links to motif diagrams (section II)
Links to sequence/motif annotated alignments (section III)
This information