ID   VIRBA_AGRT5             Reviewed;         377 AA.
AC   P17800;
DT   01-AUG-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1990, sequence version 2.
DT   14-MAY-2014, entry version 94.
DE   RecName: Full=Protein virB10;
GN   Name=virB10; OrderedLocusNames=Atu6176; ORFNames=AGR_pTi_13;
OS   Agrobacterium tumefaciens (strain C58 / ATCC 33970).
OG   Plasmid pTiC58.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales;
OC   Rhizobiaceae; Rhizobium/Agrobacterium group; Agrobacterium;
OC   Agrobacterium tumefaciens complex.
OX   NCBI_TaxID=176299;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2370849; DOI=10.1007/BF00261729;
RA   Kuldau G.A., de Vos G., Owen J., McCaffrey G., Zambryski P.;
RT   "The virB operon of Agrobacterium tumefaciens pTiC58 encodes 11 open
RT   reading frames.";
RL   Mol. Gen. Genet. 221:256-266(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2194232; DOI=10.1016/0147-619X(90)90028-B;
RA   Rogowsky P.M., Powell B.S., Shirasu K., Lin T.-S., Morel P.,
RA   Zyprian E.M., Steck T.R., Kado C.I.;
RT   "Molecular characterization of the vir regulon of Agrobacterium
RT   tumefaciens: complete nucleotide sequence and gene organization of the
RT   28.63-kbp regulon cloned as a single unit.";
RL   Plasmid 23:85-106(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2233252; DOI=10.1111/j.1365-2958.1990.tb00690.x;
RA   Shirasu K., Morel P., Kado C.I.;
RT   "Characterization of the virB operon of an Agrobacterium tumefaciens
RT   Ti plasmid: nucleotide sequence and protein analysis.";
RL   Mol. Microbiol. 4:1153-1163(1990).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11743193; DOI=10.1126/science.1066804;
RA   Wood D.W., Setubal J.C., Kaul R., Monks D.E., Kitajima J.P.,
RA   Okura V.K., Zhou Y., Chen L., Wood G.E., Almeida N.F. Jr., Woo L.,
RA   Chen Y., Paulsen I.T., Eisen J.A., Karp P.D., Bovee D. Sr.,
RA   Chapman P., Clendenning J., Deatherage G., Gillet W., Grant C.,
RA   Kutyavin T., Levy R., Li M.-J., McClelland E., Palmieri A.,
RA   Raymond C., Rouse G., Saenphimmachak C., Wu Z., Romero P., Gordon D.,
RA   Zhang S., Yoo H., Tao Y., Biddle P., Jung M., Krespan W., Perry M.,
RA   Gordon-Kamm B., Liao L., Kim S., Hendrick C., Zhao Z.-Y., Dolan M.,
RA   Chumley F., Tingey S.V., Tomb J.-F., Gordon M.P., Olson M.V.,
RA   Nester E.W.;
RT   "The genome of the natural genetic engineer Agrobacterium tumefaciens
RT   C58.";
RL   Science 294:2317-2323(2001).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C58 / ATCC 33970;
RX   PubMed=11743194; DOI=10.1126/science.1066803;
RA   Goodner B., Hinkle G., Gattung S., Miller N., Blanchard M.,
RA   Qurollo B., Goldman B.S., Cao Y., Askenazi M., Halling C., Mullin L.,
RA   Houmiel K., Gordon J., Vaudin M., Iartchouk O., Epp A., Liu F.,
RA   Wollam C., Allinger M., Doughty D., Scott C., Lappas C., Markelz B.,
RA   Flanagan C., Crowell C., Gurson J., Lomo C., Sear C., Strub G.,
RA   Cielo C., Slater S.;
RT   "Genome sequence of the plant pathogen and biotechnology agent
RT   Agrobacterium tumefaciens C58.";
RL   Science 294:2323-2328(2001).
CC   -!- FUNCTION: VirB proteins are suggested to act at the bacterial
CC       surface and there play an important role in directing T-DNA
CC       transfer to plant cells.
CC   -!- INTERACTION:
CC       Self; NbExp=3; IntAct=EBI-2013080, EBI-2013080;
CC       P17798:virB8; NbExp=5; IntAct=EBI-2013080, EBI-2012972;
CC       P17799:virB9; NbExp=3; IntAct=EBI-2013080, EBI-2012881;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Single-pass membrane
CC       protein (Potential).
CC   -!- SIMILARITY: Belongs to the TrbI/VirB10 family.
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DR   EMBL; X53264; CAA37363.1; -; Genomic_DNA.
DR   EMBL; J03320; AAA91600.1; -; Genomic_DNA.
DR   EMBL; AE007871; AAK90938.1; -; Genomic_DNA.
DR   PIR; AF3249; AF3249.
DR   PIR; S12350; B0AG58.
DR   RefSeq; NP_396497.1; NC_003065.3.
DR   ProteinModelPortal; P17800; -.
DR   IntAct; P17800; 2.
DR   MINT; MINT-119421; -.
DR   STRING; 176299.Atu6176; -.
DR   EnsemblBacteria; AAK90938; AAK90938; Atu6176.
DR   GeneID; 1137499; -.
DR   KEGG; atu:Atu6176; -.
DR   PATRIC; 20820552; VBIAgrTum91616_5370.
DR   eggNOG; COG2948; -.
DR   HOGENOM; HOG000119823; -.
DR   KO; K03195; -.
DR   OMA; AGYVKCV; -.
DR   OrthoDB; EOG654NZP; -.
DR   BioCyc; AGRO:ATU6176-MONOMER; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0043684; C:type IV secretion system complex; IGC:PAMGO_GAT.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0030255; P:protein secretion by the type IV secretion system; IGC:PAMGO_GAT.
DR   InterPro; IPR005498; T4SS_VirB10/TraB/TrbI.
DR   Pfam; PF03743; TrbI; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Complete proteome;
KW   Crown gall tumor; Membrane; Plasmid; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN         1    377       Protein virB10.
FT                                /FTId=PRO_0000065846.
FT   TRANSMEM     33     50       Helical; (Potential).
FT   CONFLICT    297    297       A -> P (in Ref. 2 and 3).
SQ   SEQUENCE   377 AA;  40555 MW;  F91BB4CFCC3FE29D CRC64;
     MNDDNQQSAH DVDASGSLVS DTHHRRLSGA QKLIVGGVVL ALSLSLIWLG GREKKENGDA
     PPSTMIATNT KPFHPAPIDV TLDPPAAQEA VQPTAPPPAR SEPERHEPRP EETPIFAYTS
     GDQGTSKRVQ QGETDRRREG NGEDSPLPKV EVSAENDLSI RMKPTELQPT RATLLPHPDF
     MVTEGTIIPC ILQTAIDTSL AGYVKCVLPW DVRGTTNNVV LLDRGTTVVG EIQRGLQQGD
     ARVFVLWDRA ETPDHAMISL ASPSADELGR SGLPGTVDNH FWQRFSGAML LSVVQGAFQA
     ASTYAGSSGG GTSFNSVQNN GEQTADTALK ATINIPPTLK KNQGDTVSIF VARDLDFSGI
     YQLRMAGRAA RGRDRRP
//