1. Plant Mol Biol. 2010 Jan;72(1-2):47-60. Epub 2009 Sep 18.

Expression of vacuolar H+-pyrophosphatase (OVP3) is under control of an
anoxia-inducible promoter in rice.

Liu Q, Zhang Q, Burton RA, Shirley NJ, Atwell BJ.

Department of Biological Sciences, Macquarie University, Sydney, NSW, 2109,
Australia.

Vacuolar H(+)-pyrophosphatase (V-PPase) expression increases in a number of
abiotic stresses and is thought to play a role in adaptation to abiotic stresses.
This paper reports on the regulation of six V-PPase genes in rice (Oryza sativa
L.) coleoptiles under anoxia, using flood tolerant and intolerant cultivars to
test our hypothesis. Quantitative PCR analysis showed that one vacuolar
H(+)-pyrophosphatase (OVP3) was induced by anoxia, particularly in flood-tolerant
rice. Regulation of OVP3 expression under anoxia was investigated by analysing
putative OVP promoters. The putative OVP3 promoter contained more previously
identified anoxia-inducible motifs than the putative promoters of the other five 
OVP genes. GUS activity in transgenic rice plants containing the OVP3 promoter
region linked to the GUS reporter gene was induced only by anoxia. Salt and cold 
treatments had little effect on OVP3 promoter-driven GUS expression when compared
to the anoxic treatment.

PMID: 19763843 [PubMed - indexed for MEDLINE]