FastQCFastQC Report
Чт 27 фев 2020
chr6.1.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenamechr6.1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences54019
Sequences flagged as poor quality0
Sequence length27-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATG578610.711046113404542No Hit
CTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAG32806.071937651567041No Hit
GGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGA26734.948258945926433No Hit
GGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGC23904.424369203428424No Hit
CTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTG22614.1855643384735No Hit
CAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAAT17413.2229400766397007No Hit
GCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCA14962.769395953275699No Hit
GCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCAT14442.673133527092319No Hit
CCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATT13152.4343286621373963No Hit
GCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAA11092.0529813584109298No Hit
GGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAG9251.7123604657620466No Hit
CAGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCT8871.6420148466280382No Hit
CATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATGCC7711.42727558821896No Hit
CCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAG7211.3347155630426333No Hit
ATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATGCCA6631.227345933838094No Hit
CCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT6611.2236435328310409No Hit
TCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT6001.1107203021159222No Hit
ATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTA5781.0699938910383384No Hit
GTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACATAGTA5681.051481886003073No Hit
CCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATGC5380.9959458708972768No Hit
GTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGTC5340.9885410688831707No Hit
CCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATA4700.8700642366574723No Hit
GGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA4550.8422962291045744No Hit
GGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGT4430.820081823062256No Hit
GCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT4320.799718617523464No Hit
CCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAA4300.796016216516411No Hit
TTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA4290.7941650160128844No Hit
CTGGCATTGCCATCCTTACGGGTGACTTTCCATCCCTTGAACCAAGGCATG4170.7719506099705659No Hit
TGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGG3780.699753790333031No Hit
GCATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCT3680.6812417852977656No Hit
TTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTC3490.6460689757307614No Hit
CGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGC3480.6442177752272349No Hit
GGAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCT3390.6275569706954961No Hit
CTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCT3360.6220033691849165No Hit
CATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAG3310.6127473666672837No Hit
GGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCC3220.5960865621355449No Hit
CACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCT3000.5553601510579611No Hit
GTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATG2940.5442529480368019No Hit
GTGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGA2930.5424017475332753No Hit
GCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCA2870.5312945445121161No Hit
CTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA2790.5164849404839038No Hit
GTCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGC2680.49612173494511186No Hit
GGACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTTCCA2670.49427053444158536No Hit
GTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA2610.48316333142042617No Hit
TGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGT2360.4368833188322627No Hit
ATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGT2300.42577611581110353No Hit
GTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTC2250.4165201132934708No Hit
GCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTT2230.4128177122864177No Hit
CTTCAAATTCACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCT2210.4091153112793647No Hit
GCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGA2180.4035617097687851No Hit
GCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATT2080.3850497047335197No Hit
GGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG2010.37209130120883394No Hit
CCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGAT1980.36653769969825434No Hit
CTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGC1970.3646864991947278No Hit
CACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCC1920.3554304966770951No Hit
CTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGCTC1870.3461744941594624No Hit
GAGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTT1870.3461744941594624No Hit
GTCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCAT1850.34247209315240934No Hit
GTCTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGC1740.32210888761361745No Hit
ACCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGAT1710.31655528610303785No Hit
CAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGTCATT1700.3147040855995113No Hit
AGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG1690.31285288509598475No Hit
ATCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGA1660.30729928358540515No Hit
GCAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCCCTGTAATCATG1630.30174568207482555No Hit
GCCTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGG1560.28878727855013975No Hit
CGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAG1540.2850848775430867No Hit
TGGACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTTCC1460.2702752735148744No Hit
GACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTTCCAC1440.2665728725078213No Hit
TGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTAT1420.26287047150076825No Hit
GTCAGCCTGAGATGTCCCTGTAATCATGTTTTTGATAAAGTCTCTGTGTCC1320.24435846646550288No Hit
CTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTC1250.2314000629408171No Hit
CCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTG1230.22769766193376406No Hit
CGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG1210.223995260926711No Hit
GCTTCAAATTCACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCC1190.22029285991965789No Hit
GCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGC1170.2165904589126048No Hit
GTGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAG1160.2147392584090783No Hit
GCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATC1100.20363205538791906No Hit
GTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCC1050.19437605287028636No Hit
TTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACATAGTACT1050.19437605287028636No Hit
GTTTGAGAACACCAGTCTCCACTCGGCCAACAGGAACAGTACCAATACCAC1000.1851200503526537No Hit
GTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCAGCAG990.18326884984912717No Hit
CATGCAATGTGAGCCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTT980.18141764934560062No Hit
ACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCCC930.17216164682796795No Hit
GATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTG890.1647568448138618No Hit
CAGCAACAATCAGGACAGCACAGTCAGCCTGAGATGTCCCTGTAATCATGT890.1647568448138618No Hit
TTCAAATTCACCAACACCAGCAGCAACAATCAGGACAGCACAGTCAGCCTG860.15920324330328217No Hit
GGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGAG830.15364964179270257No Hit
TCACGCTCAGCTTTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCC810.14994724078564947No Hit
TTCAGTTTATCCAAGACCCAGGCATACTTGAAGGAGCCCTTTCCCATCTCA810.14994724078564947No Hit
ATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAAGCTCTCAAC770.14254243877154335No Hit
GTCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCA760.1406912382680168No Hit
CATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACATA740.13698883726096373No Hit
CACCTGAGCAGTGAAGCCAGCTGCTTCCATTGGTGGGTCATTTTTGCTGTC740.13698883726096373No Hit
CGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGATG730.1351376367574372No Hit
CGGTAATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAG690.12773283474333105No Hit
GGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG680.1258816342398045No Hit
CGCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGTCGATG650.1203280327292249No Hit
GTTGGACGAGTTGGTGGTAGGATGCAGTCCAGAGCCTCAAGCAGCGTGGTT650.1203280327292249No Hit
AGCCCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTT640.11847683222569835No Hit
CTGTCTCATATCACGAACAGCAAAGCGACCCAAAGGTGGATAGTCTGAGAA640.11847683222569835No Hit
CCGTGTGGCAATCCAATACAGGGGCATAGCCGGCGCTTATTTGGCCTGGAT640.11847683222569835No Hit
CCTTTCCCATCTCAGCAGCCTCCTTCTCAAATTTTTCAATGGTTCTTTTGT640.11847683222569835No Hit
CGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTGA610.11292323071511876No Hit
ATCATGTTTTTGATAAAGTCTCTGTGTCCTGGGGCATCAATGATAGTCACA610.11292323071511876No Hit
TGGGGCATAGCCGGCGCTTATTTGGCCTGGATGGTTCAGGATAATCACCTG590.10922082970806568No Hit
CCTTGAACCAAGGCATGTTAGCACTTGGCTCCAGCATGTTGTCACCATTCC580.10736962920453914No Hit
CGGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCCATTG570.10551842870101259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGCCA700.045.5731745
TGCATAG253.8503524E-544.982412
GTGCATA253.8503524E-544.982411
GTCATAG351.1870725E-744.982411
CTCCCAT253.8503524E-544.982411
TCATAGC351.1870725E-744.982412
ATCCCAT206.9871097E-444.9824071
TGGCATT406.6320354E-944.9824072
TGGCATA453.7107384E-1044.9824072
CGGGGCA206.9871097E-444.9824071
CTGGCAT406.6320354E-944.9824071
GTCCCAT206.9871097E-444.9824071
GTGGCAT453.7107384E-1044.9824071
CCCCATC800.044.9824071
GGCATTG451.8770152E-839.9843643
TCCTTAC504.749745E-835.98592813
CATTGCC504.749745E-835.9859285
GCCCATC504.749745E-835.9859281
CATCCTT504.749745E-835.98592811
GGGGGCA250.002093426735.9859281
Produced by FastQC (version 0.11.2)