LOCUS       LACSS45BB                576 bp    mRNA    linear   BCT 23-MAR-1995
DEFINITION  Lactococcus lactis (clone ss45) beta-lactamase gene, 5' end.
ACCESSION   M63294
VERSION     M63294.1
KEYWORDS    beta-lactamase.
SOURCE      Lactococcus lactis
  ORGANISM  Lactococcus lactis
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Streptococcaceae;
            Lactococcus.
REFERENCE   1  (bases 1 to 576)
  AUTHORS   Sibakov,M., Koivula,T., von Wright,A. and Palva,I.
  TITLE     Secretion of TEM beta-lactamase with signal sequences isolated from
            the chromosome of Lactococcus lactis subsp. lactis
  JOURNAL   Appl. Environ. Microbiol. 57 (2), 341-348 (1991)
   PUBMED   1901704
COMMENT     On Mar 24, 1995 this sequence version replaced gi:149507.
            Original source text: L.lactis, cDNA to mRNA, clone ss45.
FEATURES             Location/Qualifiers
     source          1..576
                     /organism="Lactococcus lactis"
                     /mol_type="mRNA"
                     /strain="MG1614"
                     /sub_species="lactis"
                     /db_xref="taxon:1358"
                     /clone="SS45"
     gene            1..576
                     /gene="beta-lactamase"
     regulatory      136..141
                     /regulatory_class="minus_35_signal"
                     /gene="beta-lactamase"
     regulatory      162..167
                     /regulatory_class="minus_10_signal"
                     /gene="beta-lactamase"
     mRNA            176..576
                     /gene="beta-lactamase"
                     /product="beta-lactamase"
     regulatory      323..330
                     /regulatory_class="ribosome_binding_site"
                     /gene="beta-lactamase"
     CDS             340..>576
                     /gene="beta-lactamase"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAA64244.1"
                     /translation="MKKINLSKLIIIALIIIIAAMSAIFISAKNFKSNKNPSAMTQTI
                     NDGTSFVDRVLVAPIHFVQDKANELSNLIIPPETL"
ORIGIN      
        1 gatagtcttt tccttcattc ttttcgccaa aaactggaat atgattcata aaaattttcc
       61 cccaaaattg atttacatga taaaagttaa atatttcatt atgtaaaaac cgcttacaaa
      121 aatttattac tactattgta tcaaaattct gtcaaatttg ataaaataag gtacgagtaa
      181 gaagaactta tacaatgatc atattgaaaa aatataattc aagaagagag atgttataag
      241 aggtattttt gcattgggca aaaagcgatg tgaaatcaca tggctttcga acctaaaaga
      301 gatgggtttg aaacgtatta ctgaagggaa cctagaatag tgaaaaaaat taacttaagt
      361 aagcttatta ttattgcact cattatcatt attgcagcga tgtcagcaat ttttatttca
      421 gccaaaaatt ttaagtccaa taaaaatccg tcagcaatga cccaaacaat taatgatggg
      481 acgagttttg ttgaccgagt tcttgttgca ccaatccatt ttgtacaaga caaagcaaat
      541 gagctttcaa acttgataat tcccccagaa acgctg
//


