AatI	NEBC13653	Type II	R	AGGCCT	274	-	-	-	-	no	-
AatII	U65398	Type II	R	GACGTC	345	-	AAC44856.1	P70725	1498744	no	-
AbrI	X62690	Type II	R	CTCGAG	222	-	CAA44567.1	P26919	38661	no	-
Acc65I	CP019041	Type II	R	GGTACC	539	BVL33_04230	APU47776.1	-	113005857	no	-
Acc65I	CP019041	Type II	R	GGTACC	539	BVL33_04230	APU47776.1	-	113005857	no	-
AhdI	NEBC14060	Type II	R	GACNNNNNGTC	314	-	-	-	-	no	-
AluI	CP041694	Type II	R	AGCT	408	FOG94_17070	QDP76585.1	D9MYV5	-	no	-
Aor13HI	NEBM12	Type II	R	TCCGGA	30	-	-	-	-	no	-
ApaI	HQ327698	Type II	R	GGGCCC	355	-	ADO24182.1	E3VXA1	308229533	no	-
AplI	AP011615	Type II	R	CTGCAG	324	NIES39_K04640	BAI92109.1	D4ZX34	291569837	no	-
AquI	CP000951	Type II	R	CYCGRG	317	SYNPCC7002_A1189	ACA99188.1	B1XKE2	169885475	no	-
AvaI	BA000019	Type II	R	CYCGRG	315	-	BAB75330.1	P0A457	17132764	no	-
AvaIII	BA000019	Type II	R	ATGCAT	381	-	BAB76109.1	Q8YNZ9	17133546	no	-
BalI	D82028	Type II	R	TGGCCA	260	-	BAA11515.1	P71102	1620877	no	-
BamFI	FN597644	Type II	R	GGATCC	213	BAMF_3131	CBI44257.1	E1UQE5	307607886	no	-
BamHI	CP041693	Type II	R	GGATCC	213	FOG69_15995	QDP93514.1	-	-	no	-
BbrUII	CP000303	Type II	R	GTCGAC	695	Bbr_1120	ABE95798.1	C1J0P6	339479330	no	-
BbrUIII	CP000303	Type II	R	CTGCAG	315	Bbr_1118	ABE95796.1	C1J0P8	339479328	no	-
BfuAII	NEBC7814	Type II	R	GCATGC	251	-	-	-	-	no	-
BglI	CP014840	Type II	R	GCCNNNNNGGC	299	A1D11_20185	AMR64562.1	-	100929146	no	-
BhaII	BA000004	Type II	R	GGCC	317	-	BAB07228.1	Q9K764	-	no	-
BsaAI	HQ446231	Type II	R	YACGTR	260	-	ADQ20494.1	E5LGA6	311977236	no	-
BsaHI	EU386360	Type II	R	GRCGYC	370	-	ABY86225.1	B0LX59	166237007	no	-
Bse634I	AY046877	Type II	R	RCCGGY	293	-	AAL03948.1	Q8RT53	18700047	no	-
Bsp98I	AJ245583	Type II	R	GGATCC	199	-	CAB52545.1	Q9RM91	5730143	no	-
BspEI	HQ636103	Type II	R	TCCGGA	300	-	ADR72990.1	E5Q8T5	313667092	no	-
BspRI	X15758	Type II	R	GGCC	304	-	CBE66553.1	C9IY17	258593907	no	-
BssHII	NEBC14846	Type II	R	GCGCGC	417	-	-	-	-	no	-
BstFI	NEBC15050	Type II	R	AAGCTT	318	-	-	-	-	no	-
BstFI	NEBC15050	Type II	R	AAGCTT	318	-	-	-	-	no	-
BstVI	L07643	Type II	R	CTCGAG	224	-	AAA22281.1	P43421	142619	no	-
BstZ1II	AY789018	Type II	R	AAGCTT	318	-	AAX14642.1	Q32WE8	60202513	no	-
BstZ1II	AY789018	Type II	R	AAGCTT	318	-	AAX14642.1	Q32WE8	60202513	no	-
BsuBI	L01541	Type II	R	CTGCAG	316	-	AAA18170.1	P33562	143055	no	-
BsuMIA	AL009126	Type II	R	CTCGAG	313	BSU_06090	CAB12428.1	O35025	-	no	BsuMI
BsuMIB	AL009126	Type II	R	CTCGAG	343	BSU_06100	CAB12429.1	O34885	-	no	BsuMI
BsuMIC	AL009126	Type II	R	CTCGAG	465	BSU_06110	CAB12430.1	O34303	-	no	BsuMI
BsuRI	X02988	Type II	R	GGCC	576	-	CAA26730.1	P06529	40246	no	-
CagI	CP001337	Type II	R	GCCNNNNNGGC	237	Cagg_3339	ACL26184.1	B8G8D0	219544446	no	-
CbeI	CP001393	Type II	R	GGCC	326	Athe_2438	ACM61506.1	B9MNH5	222457244	no	-
Cfr10I	AY046876	Type II	R	RCCGGY	285	-	AAL03946.1	Q79SA1	18700044	no	-
Cfr42I	EU262735	Type II	R	CCGCGG	216	-	ABY83632.1	B0LL99	166163614	no	-
Cfr9I	X17022	Type II	R	CCCGGG	330	-	CAA34888.1	Q60132	40465	no	-
ClaI	JF323047	Type II	R	ATCGAT	359	-	ADX97300.1	F1KC47	323650446	no	-
CphBI	CP001101	Type II	R	CCCGGG	257	Cphamn1_1063	ACE04003.1	B3EQH0	189495455	no	-
Csp231I	AY787793	Type II	R	AAGCTT	313	-	AAX19733.1	Q32WH3	60418613	no	-
Csp231I	AY787793	Type II	R	AAGCTT	313	-	AAX19733.1	Q32WH3	60418613	no	-
Csp68KII	CP000806	Type II	R	TTCGAA	219	cce_2281	ACB51631.1	B1WPR1	171698650	no	-
Csp68KIII	CP000806	Type II	R	ATGCAT	373	cce_1833	ACB51183.1	B1WZN1	171698202	no	-
CviQI	DQ491002	Type II	R	GTAC	298	-	-	-	-	no	-
DdsI	CP001358	Type II	R	GGATCC	201	Ddes_2242	ACL50137.1	B8J492	-	no	-
EaeI	AF039582	Type II	R	YGGCCR	315	-	AAB95338.1	O52511	2760956	no	-
EagI	EU371940	Type II	R	CGGCCG	301	-	ABY76238.1	B0LT74	165973117	no	-
Eco1524I	EU863203	Type II	R	AGGCCT	272	-	-	-	-	no	-
Eco29kI	AJ001708	Type II	R	CCGCGG	214	-	CAA04943.1	Q46944	2660512	no	-
Eco47II	X82105	Type II	R	GGNCC	239	-	CAA57628.1	P50195	558570	no	-
EcoGIII	AFST01000004	Type II	R	CTGCAG	317	C22711_2126	EGT68097.1	-	341918483	no	-
EcoHK31I	X82231	Type II	R	YGGCCR	318	-	CAA57707.1	O87741	3660495	no	-
EcoO109I	AF157599	Type II	R	RGGNCCY	272	-	AAF06964.1	Q9RPJ3	6318607	no	-
EcoRI	J01675	Type II	R	GAATTC	277	-	AAA26371.1	P00642	152447	no	-
EcoVIII	AF158026	Type II	R	AAGCTT	307	-	AAF71176.1	Q9JP70	7960014	no	-
EcoVIII	AF158026	Type II	R	AAGCTT	307	-	AAF71176.1	Q9JP70	7960014	no	-
EsaWC1I	AY588130	Type II	R	GGCC	129	-	AAS99731.1	Q6PPG6	46518734	no	-
FnuDI	AF051374	Type II	R	GGCC	284	-	AAC05694.1	O68582	2961230	no	-
FseI	JF323049	Type II	R	GGCCGGCC	219	-	ADX97304.1	F1KC51	323650452	no	-
FseI	JF323049	Type II	R	GGCCGGCC	219	-	ADX97304.1	F1KC51	323650452	no	-
FspI	JF323052	Type II	R	TGCGCA	249	-	ADX97311.1	F1KC58	-	no	-
HaeIII	AF051375	Type II	R	GGCC	318	-	AAC05697.1	O68584	2961234	no	-
HgiCI	X55138	Type II	R	GGYRCC	345	-	CAA38932.1	P25258	581260	no	-
HgiDI	X55140	Type II	R	GRCGYC	359	-	CAA38937.1	P24599	43481	no	-
HgiDII	X55141	Type II	R	GTCGAC	611	-	CAA38942.1	P25280	48774	no	-
HgiGI	X55143	Type II	R	GRCGYC	335	-	-	-	-	no	-
HindIII	L42023	Type II	R	AAGCTT	300	HI_1393	AAC23040.1	P43870	1574230	no	-
HindIII	L42023	Type II	R	AAGCTT	300	HI_1393	AAC23040.1	P43870	1574230	no	-
Hpy303I	FJ233114	Type II	R	GTAC	251	-	ACI43076.1	B6ED52	209361011	no	-
Hpy312I	FJ233115	Type II	R	GTAC	251	-	ACI43077.1	B6ED52	209361013	no	-
Hpy401I	FJ233128	Type II	R	GTAC	251	-	ACI43090.1	B6ED66	209361039	no	-
Hpy421I	FJ233126	Type II	R	GTAC	251	-	ACI43088.1	B6ED56	209361035	no	-
Hpy423I	FJ233118	Type II	R	GTAC	251	-	ACI43080.1	B6ED56	209361019	no	-
Hpy471I	FJ233120	Type II	R	GTAC	251	-	ACI43082.1	B6ED58	209361023	no	-
Hpy501I	FJ233123	Type II	R	GTAC	251	-	ACI43085.1	B6ED61	209361029	no	-
HpyHII	CP000241	Type II	R	GTAC	251	HPAG1_0479	ABF84546.1	Q1CU26	107836677	no	-
HpyNSH57I	FJ233108	Type II	R	GTAC	212	-	ACI43070.1	B6ED46	209360999	no	-
KasI	JF323050	Type II	R	GGCGCC	285	-	ADX97306.1	F1KC53	323650455	no	-
Kpn2I	Y19109	Type II	R	TCCGGA	301	-	CAC41107.1	Q93K38	14330658	no	-
KpnI	X61796	Type II	R	GGTACC	218	-	CAA43897.1	P25237	43888	no	-
KpnI	X61796	Type II	R	GGTACC	218	-	CAA43897.1	P25237	43888	no	-
LlaCI	HQ646604	Type II	R	AAGCTT	332	-	AEV59860.1	G9BNL2	359755276	no	-
LlaCI	HQ646604	Type II	R	AAGCTT	332	-	AEV59860.1	G9BNL2	359755276	no	-
LlaDI	AJ292520	Type II	R	AGTACT	251	-	CAC44540.1	Q93K18	15020665	no	-
LlaDI	AJ292520	Type II	R	AGTACT	251	-	CAC44540.1	Q93K18	15020665	no	-
LraI	LT222052	Type II	R	GAATTC	278	-	-	-	-	no	-
McaTI	AE017282	Type II	R	GCGCGC	340	MCA1617	AAU92380.1	Q607Y4	53758089	no	-
MjaII	L77117	Type II	R	GGNCC	370	MJ_1449	AAB99461.1	Q58844	1592094	no	-
MjaV	L77117	Type II	R	GTAC	230	MJ_1500	AAB99521.1	Q58895	2826424	no	-
MluI	JF323053	Type II	R	ACGCGT	276	-	AEN71951.1	G3EST6	345106999	no	-
MscI	JF432060	Type II	R	TGGCCA	261	-	ADZ31426.1	F1KM51	325564204	no	-
MspA1I	AY466402	Type II	R	CMGCKG	266	-	AAR30095.1	Q6S4U8	39726295	no	-
MthTI	X68366	Type II	R	GGCC	281	-	CAA48435.1	P29565	44640	no	-
NaeI	U09581	Type II	R	GCCGGC	317	-	AAC43324.1	P50187	775102	no	-
NgoAII	AE004969	Type II	R	GGCC	278	-	-	-	-	no	-
NgoAIII	AE004969	Type II	R	CCGCGG	213	-	-	-	-	no	-
NgoAIV	AE004969	Type II	R	GCCGGC	286	NGO_0874	AAW89575.1	Q5F8B2	59718170	no	-
NgoBV	U43735	Type II	R	GGNNCC	175	-	AAA86269.1	Q50975	1165243	no	-
NgoMIII	CP003909	Type II	R	CCGCGG	213	NGFG_02013	EEZ48556.1	-	268583880	no	-
NgoMIV	CP003909	Type II	R	GCCGGC	286	NGFG_00854	EEZ47687.1	-	268583011	no	-
NgoMV	CP003909	Type II	R	GGNNCC	243	NGFG_01122	EEZ47955.1	-	268583279	no	-
NgoPII	X52661	Type II	R	GGCC	278	-	CAA36887.1	P24617	44887	no	-
NgoSII	U42460	Type II	R	GGCC	212	-	AAB03208.1	Q79CC3	1399075	no	-
NheI	AY462277	Type II	R	GCTAGC	328	-	AAR23810.1	Q6S994	38564763	no	-
NlaIV	U06074	Type II	R	GGNNCC	243	-	AAA53238.1	P50183	476228	no	-
NmeDI	AJ238948	Type II	R	RCCGGY	351	-	CAB59898.1	Q9RLM3	6179653	no	-
NmeSI	AF123569	Type II	R	AGTACT	199	-	AAD39135.2	Q9XCX4	28212209	no	-
NmeSI	AF123569	Type II	R	AGTACT	199	-	AAD39135.2	Q9XCX4	28212209	no	-
Nno22aORF54440P	CP006850	Type II	R	GTCGAC	307	NONO_c54450	AHH20225.1	W5TLJ5	576087835	yes	-
NsoJS138I	CP007035	Type II	R	CAGCTG	237	-	-	-	-	no	-
NsoJS138I	CP007035	Type II	R	CAGCTG	237	-	-	-	-	no	-
NspHI	AF056037	Type II	R	RCATGY	244	-	AAC97193.1	Q9ZHP0	4033741	no	-
NspI	AF056036	Type II	R	RCATGY	244	-	AAC97191.1	Q9ZHP2	4033738	no	-
NspIII	E13536	Type II	R	CYCGRG	322	-	-	-	-	no	-
NspV	D14719	Type II	R	TTCGAA	220	-	BAA03538.1	P35677	442473	no	-
PabI	AL096836	Type II	R	GTAC	226	-	CAB49082.1	Q9V2B6	5457591	no	-
Pac25I	U88088	Type II	R	CCCGGG	333	-	AAD40333.1	Q9XAW7	5123479	no	-
PaeR7I	X03274	Type II	R	CTCGAG	246	-	CAA27026.1	P05104	45387	no	-
PciI	JX481785	Type II	R	ACATGT	257	-	AFV09321.1	K4LNB0	409034609	no	-
PhoI	BA000001	Type II	R	GGCC	316	-	-	-	-	no	-
PluTI	BX470251	Type II	R	GGCGCC	331	plu0599	CAE12894.1	Q7N8W6	36784025	no	-
PspPI	Y07554	Type II	R	GGNCC	32	-	-	-	-	no	-
R1.BbrUI	CP000303	Type II	R	GGCGCC	282	-	-	-	-	no	-
R1.BsuMI	CP010052	Type II	R	CTCGAG	313	QU35_03530	AIY91901.1	-	728883506	no	-
R2.BsuMI	CP010052	Type II	R	CTCGAG	343	QU35_03535	AIY91902.1	-	728883507	no	-
R3.BsuMI	CP010052	Type II	R	CTCGAG	465	QU35_03540	AIY91903.1	-	728883508	no	-
RflFI	CC820644	Type II	R	GTCGAC	126	-	-	-	-	no	-
RrhJ1I	DJ340847	Type II	R	GCCGGC	308	-	-	-	-	no	-
RsaI	CP020470	Type II	R	GTAC	160	B6K69_04315	AWD20989.1	-	-	no	-
RspRSI	CP000686	Type II	R	GCCNNNNNGGC	236	RoseRS_4185	ABQ92529.1	A5V0X6	148570384	no	-
RsrI	X14697	Type II	R	GAATTC	277	-	CAA32827.1	P21763	46453	no	-
SacI	AF027867	Type II	R	GAGCTC	358	-	AAC97119.1	O31074	2605795	no	-
SacI	AF027867	Type II	R	GAGCTC	358	-	AAC97119.1	O31074	2605795	no	-
SalI	U01232	Type II	R	GTCGAC	315	-	AAA81886.1	Q53608	402237	no	-
Sau96I	X53096	Type II	R	GGNCC	261	-	CAA37259.1	P23736	46618	no	-
SbaI	AF508974	Type II	R	CAGCTG	187	-	AAM93163.1	Q8KT01	22135358	no	-
SbaI	AF508974	Type II	R	CAGCTG	187	-	AAM93163.1	Q8KT01	22135358	no	-
SbfI	DQ026300	Type II	R	CCTGCAGG	323	-	AAY33699.1	Q4TZJ3	63109085	no	-
ScaI	NEBM97	Type II	R	AGTACT	227	-	-	-	-	no	-
ScaI	NEBM97	Type II	R	AGTACT	227	-	-	-	-	no	-
SdaI	FJ905181	Type II	R	CCTGCAGG	323	-	ACQ73748.1	C4P954	229488228	no	-
SenpCI	AY079201	Type II	R	CCGCGG	214	-	AAL87018.1	Q8RLL0	19526585	no	-
SfiI	AF039750	Type II	R	GGCCNNNNNGGCC	269	-	AAB95365.1	O52512	2761009	no	-
SfiI	AF039750	Type II	R	GGCCNNNNNGGCC	269	-	AAB95365.1	O52512	2761009	no	-
SghWII	ABYA01000270	Type II	R	GCCGGC	401	-	-	-	-	no	-
Sgr13350I	AP009493	Type II	R	GAGCTC	397	SGR_3359	BAG20188.1	B1VM78	178465668	no	-
Sgr13350I	AP009493	Type II	R	GAGCTC	397	SGR_3359	BAG20188.1	B1VM78	178465668	no	-
SgrAI	NEBC15354	Type II	R	CRCCGGYG	345	-	-	-	-	no	-
SgrAI	NEBC15354	Type II	R	CRCCGGYG	345	-	-	-	-	no	-
SmaI	X16458	Type II	R	CCCGGG	247	-	CAA34478.1	P14229	47264	no	-
SnaBI	AY461517	Type II	R	TACGTA	223	-	AAS19439.1	Q6SA25	42541082	no	-
SonI	AE014300	Type II	R	ATCGAT	348	SO_A0003	AAN52942.1	Q8E8A6	24344699	no	-
SpeI	NEBC15359	Type II	R	ACTAGT	208	-	-	-	-	no	-
SphI	U33216	Type II	R	GCATGC	235	-	AAB40378.1	P95821	1773270	no	-
SsoI	M97479	Type II	R	GAATTC	277	-	AAA25653.1	Q6LDF2	150854	no	-
SuaI	CP000077	Type II	R	GGCC	371	Saci_1976	AAY81278.1	Q4J7F1	68568349	no	-
TdeIII	AE017226	Type II	R	GGNCC	258	TDE_0911	AAS11402.1	Q73P88	41816616	no	-
TliI	CP006670	Type II	R	CTCGAG	281	OCC_13281	EHR77447.1	H3ZRP1	374741014	no	-
TspMI	EF426476	Type II	R	CCCGGG	379	-	ABO38150.1	A4L311	133753294	no	-
Tth111I	JN035226	Type II	R	GACNNNGTC	319	-	AEN19710.1	G3JXC7	344915419	no	-
Tvu2HI	CP039710	Type II	R	GGCC	316	FA954_10130	QCV55937.1	-	-	no	-
Van91II	NEBM49	Type II	R	GAATTC	267	-	-	-	-	no	-
XamI	U77781	Type II	R	GTCGAC	274	-	AAD13687.1	P96189	1688236	no	-
XcyI	M98768	Type II	R	CCCGGG	333	-	AAA27609.1	P30773	155372	no	-
XhoI	NEBC13645	Type II	R	CTCGAG	246	-	-	-	-	no	-
XmaIII	NEBC13654	Type II	R	CGGCCG	300	-	-	-	-	no	-
XmnI	NEBC13638	Type II	R	GAANNNNTTC	374	-	-	-	-	no	-
XorKII	AE013598	Type II	R	CTGCAG	364	XOO4914	ABJ90022.1	Q05I80	116247096	no	-
XphI	AF042157	Type II	R	CTGCAG	364	-	AAF22367.1	Q9RBJ6	6652556	no	-
XveI	DQ256492	Type II	R	CTGCAG	364	-	ABB80538.1	Q2Q0I6	82504503	no	-