TBLASTN 2.2.28+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: sa_genome.fasta 14 sequences; 2,212,135 total letters Query= sp|P25052|TENA_BACSU Thiaminase-2 OS=Bacillus subtilis (strain 168) GN=tenA PE=1 SV=1 Length=236 Score E Sequences producing significant alignments: (Bits) Value embl|AL766847|AL766847 Streptococcus agalactiae NEM316 complete... 70.9 6e-15 > embl|AL766847|AL766847 Streptococcus agalactiae NEM316 complete genome, segment 5 Length=167050 Score = 70.9 bits (172), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 53/213 (25%), Positives = 94/213 (44%), Gaps = 21/213 (10%) Frame = +1 Query 3 FSEECRSAAAEWWEGSFVHPFVQGIGDGTLPIDRFKYYVLQDSYYLTHFAKVQSFGAAYA 62 +E + A++ + + PF+QGI G L D +Y+ D+ YL FA + + A + Sbjct 97660 LKDEMIAKGAKYIQSIYQDPFIQGIIKGRLDHDVICHYLQADNIYLGKFADIYALCLAKS 97839 Query 63 KDL-----------YTTGRMASHAQGTYEAEMALHRefaelleiseeeRKAFKPSPTAYS 111 +L +T R + +G ++A A + + P+A Sbjct 97840 DNLRDKQFFLEQIDFTLNRELADGEGPHQALAAYTNRSYQDIIEKGVWY------PSADH 98001 Query 112 YTSHMYRSVLSGNFAEILAALLPCYWLYYEVGEKLLHCDP---GHPIYQKWIGTYGGDWF 168 Y HMY A LAA+ PC W+Y+++ +K++ + G+P + WI Y D Sbjct 98002 YIKHMYFHFYENGIAGALAAMSPCPWIYHQLAKKIIEENQFLNGNP-FNNWITFYANDTV 98178 Query 169 RQQVEEQINRFDELAENSTEEVRAKMKENFVIS 201 + +E D A+N ++E +A + + FV S Sbjct 98179 EELMENYFRMMDYYAQNLSKEKQADLVDAFVKS 98277 Lambda K H a alpha 0.319 0.134 0.429 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 115594704 Database: sa_genome.fasta Posted date: Nov 27, 2013 12:05 AM Number of letters in database: 2,212,135 Number of sequences in database: 14 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40