FastQCFastQC Report
Вт 9 дек 2014
Ath_tae_008.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAth_tae_008.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACG6400.031.447859
CACGCGT3300.023.57116912-13
ACGCGTC3250.022.5259312-13
TGCCGTC9000.022.08397548-49
TATGCCG9700.021.3713246-47
GCACGCG4100.021.20163510-11
GCGACAC4106.687881E-420.5585276
CGTATGC10550.020.05410244-45
CGCGTCT4450.018.9182384
AGAGCAC18900.018.3368478
GCCGTCT9500.017.54719748-49
GCGTCTG4451.0913936E-1116.45592914-15
ACCAACG9651.8189894E-1216.1164679
GTATGCC10700.015.97815844-45
GGCGACA5307.174604E-415.8998455
GTAGCCG6352.2531822E-715.8477349
ATGCCGT10800.015.23695946-47
CGTCTGA14600.015.10280716-17
AGTCACC14800.014.89451828-29
CATACGC5701.2781711E-614.7695024
Produced by FastQC (version 0.11.2)