#2
seqretsplit -sequence to_split.fasta -auto
#4
transeq -sequence coding.fasta protein.fasta -frame 1 -table 11
#5
getorf coding.fasta file.orf -minsize 230 -table 11
#11
makenucseq -amount 3 -length 100 -outseq 3_seq.fasta -auto
#14
degapseq alignment.fasta wi_ali.fasta