FastQCFastQC Report
Пн 19 дек 2016
SRR4240384.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4240384.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9825592
Sequences flagged as poor quality0
Sequence length39
%GC30

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ANNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN416420.4238116135903058No Hit
TNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN342150.3482232928051562No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA276700.2816115303790347No Hit
GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN177970.181129035278485No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN162980.16587295706966051No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTCG7700.017.9628319
TCGGAAG8700.017.7551273
GATCGGA9300.016.5409261
TGCCGTC6650.016.1725118
AGAGCTC9250.015.0505298
TATGCCG8000.014.68243116
GCCGTCT8200.014.33049419
GCTCGTA9950.014.27039811
CGGAAGA11150.014.1641714
ATGCCGT7950.013.73555417
CGTATGC9000.013.60587214
GTATGCC10200.013.13260315
GAAGAGC12200.012.9771646
ATCGGAA12450.012.6209852
AGCTCGT10850.012.60936610
CCGTCTT10350.012.16326920
GGAAGAG14950.010.7974595
TCGTATG13450.010.09306113
CGTCTTC12150.09.82137121
AAGAGCT17450.09.3716347