TBLASTN 2.9.0+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= sp|Q8SR66|MCES_ENCCU mRNA cap guanine-N7 methyltransferase OS=Encephalitozoon cuniculi (strain GB-M1) OX=284813 GN=ABD1 PE=1 SV=2 Length=283 Score E Sequences producing significant alignments: (Bits) Value scaffold-550 66.2 3e-12 scaffold-17 58.2 2e-09 >scaffold-550 Length=58790 Score = 66.2 bits (160), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 70/243 (29%), Positives = 109/243 (45%), Gaps = 31/243 (13%) Frame = +3 Query 5 KEEIREHYNSIRERGRESRQRSKTINIRNANNFIKACLIRLYTKRGDSVldlgcgkggdl 64 +++ HYN + G E R +S + ++N NNFIKA L+ Y VLDL CGKGGDL Sbjct 47988 PKDVVHHYN*RPQVGTE*R*QSPIVRLKNFNNFIKASLVGDYVSPDFVVLDLCCGKGGDL 48167 Query 65 lkYERAGIGEYYGVDIAEVSINDARVRARNMKRRFKVFFRAQDSYGRH---------MDL 115 K+ + GVD++ V++ A R +N + F A D Y H + Sbjct 48168 *KFAAQEVKRVVGVDVSPVAVEHAT*RYKNPLGKNSFNF*A-DFYTLHCFERKLTEVIPA 48344 Query 116 GKEFDVISSQFSFHYAFSTSESLDIAQRNIARHLRPGGYFIMTVPSRDVIL--------- 166 D + F+ HYAF + ES+ + N + +L+ GG+F T+P+ + ++ Sbjct 48345 MPPVDAVCC*FALHYAFYSEESVRMLLHNASAYLKSGGFFFGTIPNANWLV*VIY*LINV 48524 Query 167 -ERYKQGR----------MSNDFYKIELEKMEDVPMESVREYRFTLLDSVNNCIEYFVDF 215 K R N Y + E+ R Y F L D++++C EY V F Sbjct 48525 HHNCKSCRKRLK*SDNFSFGNKIYNVRFEEKYHFKTFGHR-YWFVLEDAIDDCPEYLVHF 48701 Query 216 TRM 218 + Sbjct 48702 PTL 48710 >scaffold-17 Length=2125590 Score = 58.2 bits (139), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 56/168 (33%), Positives = 85/168 (51%), Gaps = 10/168 (6%) Frame = +3 Query 5 KEEIREHYNSIRERGRESRQRSKTINIRNANNFIKACLIRLYTKRGDSVldlgcgkggdl 64 +++ HYN G E R +S + ++N NNFIKA L+ Y R VLDL CGKGGDL Sbjct 1958667 PKDVVHHYN*RP*VGAE*R*QSPIVRLKNFNNFIKASLVGDYVSRDFVVLDLCCGKGGDL 1958846 Query 65 lkYERAGIGEYYGVDIAEVSINDARVRARNMKRRFKVFFRAQDSYGRH---------MDL 115 K+ + GVD++ V+I A R +N + F A+ Y H + Sbjct 1958847 *KFAAQEVKRVIGVDVSPVAIEHAT*RYKNPLGKNSFNF*AE-FYTLHCFERKLTEVIPA 1959023 Query 116 GKEFDVISSQFSFHYAFSTSESLDIAQRNIARHLRPGGYFIMTVPSRD 163 D + F+ HYAF + ES+ + N + +L+ GG+F T+P+ + Sbjct 1959024 MPPVDAVCC*FALHYAFYSEESVRMLLHNASAYLKSGGFFFGTIPNAN 1959167 Lambda K H a alpha 0.322 0.139 0.396 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 1451950410 Database: X5.fasta Posted date: Nov 10, 2021 1:51 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40