seqret embl:KM577634[68:1025] rat.fa cat rat.fa >> rna12.fasta muscle -align rna12.fasta -output rna12.afa python reformat.py fastme -d -i rna12.phy -o rna12.tre fastme -d -i rna12.phy -o rna12_bootstrap.tre -b 100 fastme -dp -i rna12.phy -o rna12_bootstrap_pdist.tre -b 100 reformat.py: from Bio import AlignIO inh = open("rna12_nei.afa", "r") outh = open("rna12_nei.phy", "w") alignment = AlignIO.parse(inh, "fasta") AlignIO.write(alignment, outh, "phylip-relaxed") inh.close() outh.close()