| Метка поля | Содержание |
Код(ы) доступа ("Accession number") | AC | P94522; Q5MPF4; Q795W7; |
Идентификатор записи в БД | ID | ABNA_BACSU |
Название (краткое описание) белка | DE | Recommended name: Arabinan endo-1,5-alpha-L-arabinosidase Short name=ABN Endo-arabinanase, Русское название: Арабинан эндо-1,5-альфа-L-арабинозидаза. Краткое описание:Арабинозидаза освобождает свободную арабинозу из высокомолекулярного арабоксилана и из олигосахаридов. |
Дата создания документа | DT | 20-JAN-2009, integrated into UniProtKB/Swiss-Prot. |
Дата последнего исправления аннотации | DT | 11-JAN-2011, entry version 62. |
Число публикаций, использованных при создании документа | RN | 6 публикаций |
Журнал и год самой поздней публикации | RL | Proc. Natl. Acad. Sci. U.S.A. (2005). |
Ключевые слова | KW | 3D-structure; Complete proteome; Direct protein sequencing;
Glycosidase; Hydrolase; Secreted; Signal. |
Что содержит поле комментариев? | CC | FUNCTION: Catalyzes the hydrolysis of the alpha-1,5-linked L-arabinofuranoside backbone.It is also active towards branched sugar beet
arabinan, but inactive towards larch wood arabinogalactan, wheat
arabinoxylan and p-nitrophenyl-alpha-L-arabinofuranoside. The
enzyme activity is progressively reduced as 1,5-alpha-chains
become shorter or more highly substituted.CATALYTIC ACTIVITY: Endohydrolysis of (1->5)-alpha-
arabinofuranosidic linkages in (1->5)-arabinans. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.5 mM for arabinotetraose (at 27 degrees Celsius and pH 7); KM=5.92 mM for linear 1,5-alpha-L-arabinan (at 37 degrees Celsius and pH 6.6); Vmax=1.31 mmol/min/mg enzyme (at 37 degrees Celsius and pH 6.6); pH dependence: Optimum pH is 6.0. At pH 4.0 the arabinosidase retains about 8% of activity; Temperature dependence: Optimum temperature is 60 degrees Celsius. At 80 degrees Celsius retains about 2% of activity; PATHWAY: Glycan metabolism; L-arabinan degradation. SUBCELLULAR LOCATION: Secreted (Potential). INDUCTION: Induced by arabinose and arabinan and repressed by glucose. SIMILARITY: Belongs to the glycosyl hydrolase 43 family. SEQUENCE CAUTION: Sequence=CAA99586.1; Type=Frameshift; Positions=308; |
Идентификаторы записей PDB | DR | PDB_ID=1UV4; |
Запрос | Число записей в SwissProt | Число записей в TrEMBL |
---|---|---|
"Arabinan endo-1,5-alpha-L-arabinosidase" | 29 | 133 |
arabinosidase | 87 | 651 |
Arabinan | 87 | 493 |
  | Метка поля | Белок 1 | Белок 2 |
Первый код доступа | AC | P94522; Q5MPF4; Q795W7; | Q4W930 |
Идентификатор последовательности в БД | ID | ABNA_BACSU | ABNC_ASPFU |
Название (краткое описание) белка | DE | Arabinan endo-1,5-alpha-L-arabinosidase Краткое описание:Арабинозидаза освобождает свободную арабинозу из высокомолекулярного арабоксилана и из олигосахаридов. | Probable arabinan endo-1,5-alpha-L-arabinosidase C. Участвует в диссимиляции полисахаридов и ксилана |
Дата создания документа | DT | 20-JAN-2009 | 15-JUN-2010 |
Дата последнего исправления аннотации | DT | 08-FEB-2011 | 08-FEB-2011 |
Название организма | OS | Bacillus subtilis | Aspergillus fumigatus (Sartorya fumigata) |
Классификация организма (список таксонов) | OC | Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. | Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;Eurotiomycetidae; Eurotiales; Trichocomaceae; Neosartorya. |
Длина последовательности | ID | 323 | 321 |
Молекулярная масса белка | SQ | 35446 | 35064 |
Число публикаций, использованных при создании документа | RN | 6 | 1 |
Журнал и год самой поздней публикации | RL | Proc. Natl. Acad. Sci. U.S.A. (2005). | "Nature" 2005 |
Описание вторичной структуры | FT |
MUTAGEN 43 43 MUTAGEN 44 44 MUTAGEN 104 104 MUTAGEN 124 124 MUTAGEN 163 163 MUTAGEN 181 181 MUTAGEN 182 182 MUTAGEN 215 215 CONFLICT 172 172 STRAND 39 41 STRAND 46 49 STRAND 54 57 STRAND 69 77 STRAND 79 81 HELIX 90 94 STRAND 104 110 STRAND 115 121 STRAND 129 131 STRAND 133 137 STRAND 145 147 STRAND 149 153 STRAND 157 159 STRAND 165 168 STRAND 174 178 STRAND 186 190 TURN 192 194 STRAND 196 198 STRAND 200 203 STRAND 214 221 STRAND 226 233 STRAND 242 246 STRAND 250 252 STRAND 272 274 STRAND 279 281 STRAND 287 289 STRAND 293 300 STRAND 307 313 | информации нет |
Ключевые слова | KW | 3D-structure; Complete proteome; Direct protein sequencing;Glycosidase; Hydrolase; Secreted; Signal. | Carbohydrate metabolism; Complete proteome; Glycoprotein; Glycosidase; Hydrolase; Polysaccharide degradation; Secreted; Signal;Xylan degradation. |
Темы, освещённые в комментариях | CC | FUNCTION: Catalyzes the hydrolysis of the alpha-1,5-linked L-arabinofuranoside backbone.It is also active towards branched sugar beet
arabinan, but inactive towards larch wood arabinogalactan, wheat
arabinoxylan and p-nitrophenyl-alpha-L-arabinofuranoside. The
enzyme activity is progressively reduced as 1,5-alpha-chains
become shorter or more highly substituted.CATALYTIC ACTIVITY: Endohydrolysis of (1->5)-alpha-
arabinofuranosidic linkages in (1->5)-arabinans. BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=3.5 mM for arabinotetraose (at 27 degrees Celsius and pH 7); KM=5.92 mM for linear 1,5-alpha-L-arabinan (at 37 degrees Celsius and pH 6.6); Vmax=1.31 mmol/min/mg enzyme (at 37 degrees Celsius and pH 6.6); pH dependence: Optimum pH is 6.0. At pH 4.0 the arabinosidase retains about 8% of activity; Temperature dependence: Optimum temperature is 60 degrees Celsius. At 80 degrees Celsius retains about 2% of activity; PATHWAY: Glycan metabolism; L-arabinan degradation. SUBCELLULAR LOCATION: Secreted (Potential). INDUCTION: Induced by arabinose and arabinan and repressed by glucose. SIMILARITY: Belongs to the glycosyl hydrolase 43 family. SEQUENCE CAUTION: Sequence=CAA99586.1; Type=Frameshift; Positions=308; |
FUNCTION: Endo-1,5-alpha-L-arabinanase involved in degradation of
pectin. Its preferred substrate is linear 1,5-alpha-L-arabinan (By
similarity).,br>
CATALYTIC ACTIVITY: Endohydrolysis of (1->5)-alpha-
arabinofuranosidic linkages in (1->5)-arabinans. PATHWAY: Glycan metabolism; L-arabinan degradation. SUBCELLULAR LOCATION: Secreted (By similarity). SIMILARITY: Belongs to the glycosyl hydrolase 43 family. SEQUENCE CAUTION: Sequence=EAL84189.1; Type=Erroneous initiation; Note=Translation N-terminally shortened; |
Особенности последовательности | FT | H->A: Decrease in binding affinity. D->A: Loss of arabinosidase activity. W->A: Significantly less active (10000- fold) than the wild-type arabinosidase against both linear arabinan and arabinooligosaccharides. F->A: Increase in binding affinity. D->A: Loss of arabinosidase activity. F->A: Significantly less active than the wild-type arabinosidase against both linear arabinan (50-fold) and the arabinooligosaccharides (1000-fold). W->A: Same binding affinity as wild-type. E->A: Loss of arabinosidase activity. G -> D (in Ref. 1; CAA99586). | SIGNAL 1 18 Potential. CHAIN 19 321 Probable arabinan endo-1,5-alpha-L- arabinosidase C. /FTId=PRO_0000394635. CARBOHYD 192 192 N-linked (GlcNAc...) (Potential). CARBOHYD 224 224 N-linked (GlcNAc...) (Potential) |
Идентификаторы записей PDB | DR | 1UV4; | нет |