FastQCFastQC Report
Ср 17 дек 2014
read.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameread.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC7750.023.29316348-49
TATGCCG9250.019.5150446-47
GAGCACA17550.018.1330559
AGAGCAC18350.017.0836688
TCGGAAG18550.016.9121673
GCCGTCT9200.016.52375448-49
ATGCCGT9450.016.08591546-47
CGTATGC10900.015.90342844-45
CACACGT13750.014.85357112-13
GCACACG10500.014.47569310-11
CGTCTGA13350.014.23154316-17
GATCGGA23350.014.0464591
CACGTCT14100.013.64289214-15
CGGAAGA23650.013.2651454
GGCCCGG2903.076423E-413.1028349
AGCACAC15750.012.66623210-11
GTATGCC11150.012.35229144-45
AGTCACC15300.012.2617328-29
ATCGGAA26350.012.0867552
ACGTCTG12400.012.06589114-15
Produced by FastQC (version 0.11.2)