FastQCFastQC Report
Чт 4 дек 2014
Ath_tae_CTTGTA_L003_R1_003.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameAth_tae_CTTGTA_L003_R1_003.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000000
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC9350.021.82056848-49
TCGGAAG18550.021.2344573
TATGCCG10400.021.21589746-47
CGTATGC10700.020.62273444-45
AGAGCAC19600.018.661618
GAGCACA19800.018.2336569
ATGCCGT10300.016.35433646-47
GATCGGA24200.016.0831321
GCCGTCT10200.016.04826748-49
GCACACG11950.015.50943710-11
ATCGGAA25100.015.1324042
CACACGT15400.015.12231112-13
CACGTCT15250.015.11598514-15
CGTCTGA15500.015.025516-17
CGGAAGA26150.014.6999164
AGTCACC16150.014.56626728-29
CCAGTCA16400.014.48911226-27
ACGTCTG13200.014.40292214-15
AGCACAC17050.013.93621710-11
GTATGCC13200.013.84088544-45