FastQCFastQC Report
Вт 9 дек 2014
trimmomatic.fastq

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmomatic.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3849626
Sequences flagged as poor quality0
Sequence length50-101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCCGCG2053.0621886E-612.57681516-17
CCCGGGG3207.9104543E-411.712918
GGCCCGG2800.00390609411.7077376
TGGCCCG3350.00116527911.183515
CGCGGCC2654.553769E-610.6137112-13
GCCGCGT2706.4690554E-59.54906316-17
GCGGCCG2809.6247044E-59.20802614-15
CGGCCGC3052.4358353E-48.45326914-15
CACGCCT4007.50001E-68.22045348-49
TCGCGGC3500.00106548177.366420312-13
GCCCTCC5853.4755067E-77.321056464-65
GCAGGAT10354.114623E-67.2395674
CCGCGTC4251.4523393E-47.166941618-19
ATCGCGG3850.00292137086.69583210-11
CGCGTCT4209.896784E-46.694396518-19
TCACGCC5651.4773837E-56.64223346-47
ATATCGC8700.00147473286.46246349
CAGGATA11251.0126769E-46.24412635
GGGGATC8650.009867235.9566962
CTCACGC5200.00166087285.863846346-47
Produced by FastQC (version 0.11.2)