TBLASTN 2.8.1+ Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Database: X5.fasta 1,868 sequences; 23,962,143 total letters Query= TBB_YEAST P02557 Tubulin beta chain (Beta-tubulin) Length=457 Score E Sequences producing significant alignments: (Bits) Value unplaced-665 680 0.0 scaffold-26 631 0.0 scaffold-57 343 3e-105 unplaced-5 343 3e-105 scaffold-423 143 5e-42 scaffold-693 28.5 7.8 scaffold-17 28.5 8.0 > unplaced-665 Length=20473 Score = 680 bits (1754), Expect = 0.0, Method: Compositional matrix adjust. Identities = 339/451 (75%), Positives = 386/451 (86%), Gaps = 22/451 (5%) Frame = -2 Query 1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLN------------ 48 MREI+H+ TG CGN IGA FWE I EHG+D NG Y G D+Q ER+N Sbjct 7236 MREIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYEGDSDLQLERINGEFLNVLFCA** 7057 Query 49 ----------VYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAG 98 VYFNEAS GK+VPR++ VDLEPGT+D+VR GNLFRPDN+IFGQS AG Sbjct 7056 AFCITLLLIVVYFNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAG 6877 Query 99 NVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQIthslgggtgsgmgtllisKIREE 158 N WAKGHYTEGAELVDSV+DV+R+EAEGCD LQGFQITHSLGGGTG+GMGTLLISKIREE Sbjct 6876 NNWAKGHYTEGAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREE 6697 Query 159 FPDRMMATFSVLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLN 218 +PDRMM TFSV+PSPK SDTVVEPYNATLSVHQLVE+SDETFCIDNEALYDIC RTLKL Sbjct 6696 YPDRMMCTFSVVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLT 6517 Query 219 QPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGS 278 P+YGDLN+LVS+VMSGVTTS+R+PGQLN+DLRKLAVN+VPFPRLHFFMVG+APLT+ GS Sbjct 6516 TPTYGDLNHLVSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGS 6337 Query 279 QSFRSLTVPELTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNS 338 Q +R+L+V ELT QMFDAKNMMAA+DPR+GRYL VAA FRGK+S+KEV+++M VQ+KNS Sbjct 6336 QQYRALSVAELTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNS 6157 Query 339 DYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWY 398 YFVEWIPNNV+TAVC + P+GL M+ATFI NST+IQELFKR+ DQFS MFKRKAFLHWY Sbjct 6156 SYFVEWIPNNVKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWY 5977 Query 399 TSEGMDELEFSEAESNMNDLVSEYQQYQEAT 429 T EGMDE+EF+EAESNMNDLVSEYQQYQ+AT Sbjct 5976 TGEGMDEMEFTEAESNMNDLVSEYQQYQDAT 5884 > scaffold-26 Length=483268 Score = 631 bits (1628), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/381 (81%), Positives = 351/381 (92%), Gaps = 0/381 (0%) Frame = -3 Query 49 VYFNEASSGKWVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTE 108 VY+NEAS GK+VPR++ VDLEPGT+D+VR GNLFRPDN+IFGQS AGN WAKGHYTE Sbjct 109793 VYYNEASGGKYVPRAVLVDLEPGTMDSVRAGPYGNLFRPDNFIFGQSGAGNNWAKGHYTE 109614 Query 109 GAELVDSVMDVIRREAEGCDSLQGFQIthslgggtgsgmgtllisKIREEFPDRMMATFS 168 GAELVDSV+DV+R+EAEGCD LQGFQITHSLGGGTG+GMGTLLISKIREE+PDRMM TFS Sbjct 109613 GAELVDSVLDVVRKEAEGCDCLQGFQITHSLGGGTGAGMGTLLISKIREEYPDRMMCTFS 109434 Query 169 VLPSPKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNL 228 V+PSPK SDTVVEPYNATLSVHQLVE+SDETFCIDNEALYDIC RTLKL P+YGDLN+L Sbjct 109433 VVPSPKVSDTVVEPYNATLSVHQLVENSDETFCIDNEALYDICFRTLKLTTPTYGDLNHL 109254 Query 229 VSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPE 288 VS+VMSGVTTS+R+PGQLN+DLRKLAVN+VPFPRLHFFMVG+APLT+ GSQ +R+L+V E Sbjct 109253 VSAVMSGVTTSIRFPGQLNADLRKLAVNMVPFPRLHFFMVGFAPLTSRGSQQYRALSVAE 109074 Query 289 LTQQMFDAKNMMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNN 348 LT QMFDAKNMMAA+DPR+GRYL VAA FRGK+S+KEV+++M VQ+KNS YFVEWIPNN Sbjct 109073 LTTQMFDAKNMMAASDPRHGRYLAVAAIFRGKMSMKEVDEQMLSVQTKNSSYFVEWIPNN 108894 Query 349 VQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEF 408 V+TAVC + P+GL M+ATFI NST+IQELFKR+ DQFS MFKRKAFLHWYT EGMDE+EF Sbjct 108893 VKTAVCDIPPKGLKMSATFIGNSTAIQELFKRISDQFSVMFKRKAFLHWYTGEGMDEMEF 108714 Query 409 SEAESNMNDLVSEYQQYQEAT 429 +EAESNMNDLVSEYQQYQ+AT Sbjct 108713 TEAESNMNDLVSEYQQYQDAT 108651 Score = 66.2 bits (160), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 31/51 (61%), Positives = 36/51 (71%), Gaps = 0/51 (0%) Frame = -2 Query 1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQKERLNVYF 51 MREI+H+ TG CGN IGA FWE I EHG+D NG Y G D+Q ER+N F Sbjct 110007 MREIVHL*TG*CGN*IGAKFWEVISDEHGIDPNGRYEGDSDLQLERINGKF 109855 > scaffold-57 Length=110052 Score = 343 bits (880), Expect = 3e-105, Method: Compositional matrix adjust. Identities = 177/434 (41%), Positives = 262/434 (60%), Gaps = 10/434 (2%) Frame = -2 Query 1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQ--KERLNVYFNEASSGK 58 MRE I I GQ G QIG A WE C EHG+ +GT + + N +F+E +GK Sbjct 88586 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGK 88407 Query 59 WVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMD 118 VPR++ DLEP +D VR LF P+ I G+ A N +A+GHYT G E++DSV++ Sbjct 88406 HVPRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDSVLE 88227 Query 119 VIRREAEGCDSLQGFQIthslgggtgsgmgtllisKIREEFPDRMMATFSVLPSPKTSDT 178 +R+ A+ C L GF + HS GGGTGSG G LL+ ++ ++ + F+V P+P+ S Sbjct 88226 RVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQVSTA 88047 Query 179 VVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTT 238 VVEPYN+ L+ H +EHSD F +DNEA+YDIC+R L + +PSY +LN L++ V+S +T Sbjct 88046 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 87867 Query 239 SLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKN 298 SLR+ G LN DL + NLVP+PR+HF + YAP+ + L+V E+TQ F+ N Sbjct 87866 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISAEKAYHEQLSVHEITQACFEPNN 87687 Query 299 MMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAV----C 354 M DPRNG+Y+ +RG V K+V + +++K + FV+W P + + Sbjct 87686 QMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 87507 Query 355 SVAPQG----LDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSE 410 +V P G + A ++N+T+I E + R+ +F M+ ++AF+HWY EGM+E EFSE Sbjct 87506 TVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 87327 Query 411 AESNMNDLVSEYQQ 424 A ++ L +Y++ Sbjct 87326 AREDLAALEKDYEE 87285 > unplaced-5 Length=12763 Score = 343 bits (879), Expect = 3e-105, Method: Compositional matrix adjust. Identities = 177/434 (41%), Positives = 262/434 (60%), Gaps = 10/434 (2%) Frame = +2 Query 1 MREIIHISTGQCGNQIGAAFWETICGEHGLDFNGTYHGHDDIQ--KERLNVYFNEASSGK 58 MRE I I GQ G QIG A WE C EHG+ +GT + + N +F+E +GK Sbjct 3257 MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGTMPSDKSVGVGDDSFNTFFSETGAGK 3436 Query 59 WVPRSINVDLEPGTIDAVRNSAIGNLFRPDNYIFGQSSAGNVWAKGHYTEGAELVDSVMD 118 VPR++ DLEP +D VR LF P+ I G+ A N +A+GHYT G E++DSV++ Sbjct 3437 HVPRTVFCDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTVGKEIIDSVLE 3616 Query 119 VIRREAEGCDSLQGFQIthslgggtgsgmgtllisKIREEFPDRMMATFSVLPSPKTSDT 178 +R+ A+ C L GF + HS GGGTGSG G LL+ ++ ++ + F+V P+P+ S Sbjct 3617 RVRKLADNCTGL*GFLVFHSFGGGTGSGFGALLLERLSVDYGKKSKLEFAVYPAPQISTA 3796 Query 179 VVEPYNATLSVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTT 238 VVEPYN+ L+ H +EHSD F +DNEA+YDIC+R L + +PSY +LN L++ V+S +T Sbjct 3797 VVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIGRPSYTNLNRLIAQVVSSITA 3976 Query 239 SLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSFRSLTVPELTQQMFDAKN 298 SLR+ G LN DL + NLVP+PR+HF + YAP+ + L+V E+TQ F+ N Sbjct 3977 SLRFDGSLNVDLTEFQTNLVPYPRIHFPLATYAPIISAEKAYHEQLSVHEITQACFEPNN 4156 Query 299 MMAAADPRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAV----C 354 M DPRNG+Y+ +RG V K+V + +++K + FV+W P + + Sbjct 4157 QMVKCDPRNGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPP 4336 Query 355 SVAPQG----LDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGMDELEFSE 410 +V P G + A ++N+T+I E + R+ +F M+ ++AF+HWY EGM+E EFSE Sbjct 4337 TVVPGGDVAKVPRAVCMLSNTTAIAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE 4516 Query 411 AESNMNDLVSEYQQ 424 A ++ L +Y++ Sbjct 4517 AREDLAALEKDYEE 4558 > scaffold-423 Length=1446679 Score = 143 bits (360), Expect(2) = 5e-42, Method: Compositional matrix adjust. Identities = 114/399 (29%), Positives = 197/399 (49%), Gaps = 44/399 (11%) Frame = -2 Query 72 TIDAVRNSAIGNLFRPDNYIFG--QSSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDS 129 I+ + S G L P+N S AGN WA G Y G L + + D++ REA+ +S Sbjct 1101363 VINNIM*SPYGLLHNPENVY*S*DGSGAGNNWASG-YQSGGRLSEDLFDILDREADNAES 1101187 Query 130 LQGFQIthslgggtgsgmgtllisKIREEFPDRMMATFSVLPS--PKTSDTVVEPYNATL 187 L+GF + HS+ GGTGSG+G+ L+ + + FP +++ T+SV PS SD VV+PYN+ L Sbjct 1101186 LEGFNLMHSIAGGTGSGLGSYLLEMLSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSVL 1101007 Query 188 SVHQLVEHSDETFCIDNEALYDICQRTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLN 247 S+ +L E++D +DN AL + Q L + S+ +N +VS++M+ + +R+P Sbjct 1101006 SLKRLTENADMVTVLDNAALNRLAQD*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTY 1100827 Query 248 SDLRKLAVNLVPFPRLHFFMVGYAPLTAIGSQSF-------------------------- 281 + + L +LVP + H+ Y+P T + + Sbjct 1100826 NSMIGLMSSLVPIRKCHYITCAYSPFTHNDLRDYAASSNVNAAASENSANAVNRSR*SLM 1100647 Query 282 RSLTVPELTQQMFDAKNMMAAAD--PRNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSD 339 + + ++ +++ K+ + + + P N +L+ G V K+V + K++ KN+ Sbjct 1100646 KKAGLSDIMRRLL*TKSRVISVENTPLNC-FLSAMTLV*GDVGAKDVHKAILKLKEKNTM 1100470 Query 340 YFVEWIPNNVQTAVCSVA-------PQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRK 392 F W P+++ V + PQ ++ +AN TS+ +LFK DQ+ + KR Sbjct 1100469 QFAPWKPSSM*INVARRSPCLEDGDPQSGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRN 1100290 Query 393 AFLHWYTSEGM--DEL-EFSEAESNMNDLVSEYQQYQEA 428 AFL Y M D L EF + ++ ++ EY ++A Sbjct 1100289 AFLE*YRKLDMFKDGLEEFDVSRESLLEVTEEYLACEQA 1100173 Score = 47.0 bits (110), Expect(2) = 5e-42, Method: Compositional matrix adjust. Identities = 23/63 (37%), Positives = 33/63 (52%), Gaps = 8/63 (13%) Frame = -1 Query 16 IGAAFWETICGEHGLDFNGTYHGHDD--------IQKERLNVYFNEASSGKWVPRSINVD 67 +G FW +C EHGL +G D +R +++F +A G ++PRSI VD Sbjct 1101622 VGMEFWAQLCAEHGLAPDGLPLTSSD****SSSPPLMDRKDIFFYQAEDGHYIPRSILVD 1101443 Query 68 LEP 70 LEP Sbjct 1101442 LEP 1101434 Score = 146 bits (368), Expect = 1e-37, Method: Compositional matrix adjust. Identities = 110/373 (29%), Positives = 191/373 (51%), Gaps = 41/373 (11%) Frame = +3 Query 95 SSAGNVWAKGHYTEGAELVDSVMDVIRREAEGCDSLQGFQIthslgggtgsgmgtllisK 154 S AGN WA G Y G L + + D++ REA+ +SL+GF + HS+ GGTGSG+G+ L+ Sbjct 190362 SGAGNNWASG-Y*SGERLSEDLFDILDREADNSESLEGFNLMHSIAGGTGSGLGSYLLEM 190538 Query 155 IREEFPDRMMATFSVLPS--PKTSDTVVEPYNATLSVHQLVEHSDETFCIDNEALYDICQ 212 + + FP +++ T+SV PS SD VV+PYN+ LS+ +L E++D +DN AL + Q Sbjct 190539 LSDRFPKKLIQTYSVFPSHTEAVSDVVVQPYNSLLSLKRLTENADMVTVLDNAALNRLAQ 190718 Query 213 RTLKLNQPSYGDLNNLVSSVMSGVTTSLRYPGQLNSDLRKLAVNLVPFPRLHFFMVGYAP 272 L + S+ +N +VS++M+ + +R+P + + L +LVP + H+ Y P Sbjct 190719 D*LHIKNASFAQINRMVSTIMATSSIQMRFPSYTYNSMIGLMSSLVPIRKCHYITCAYTP 190898 Query 273 LTAIGSQSFRSLT-------------------------VPELTQQMFDAKNMMAAAD--P 305 T + + +++ + ++ +++ K+ + + + P Sbjct 190899 FTHNDLRDYAAISNVNAASENSTNAVNRSR*SLMKKAGLSDIMRRLL*PKSRVISVENTP 191078 Query 306 RNGRYLTVAAFFRGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQ------ 359 N YL+ + +G V K+V + K++ KN+ F W P+++ V +P Sbjct 191079 LNC-YLSAMSIVQGDVGAKDVHKAILKLKEKNTMQFAPWKPSSM*INVARRSPCLEDGDT 191255 Query 360 -GLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLHWYTSEGM--DEL-EFSEAESNM 415 G ++ +AN TS+ +LFK DQ+ + KR AFL Y M D L EF + ++ Sbjct 191256 *GGRVSGLMLANHTSVAQLFKSTIDQYDRLRKRNAFLE*YRKLDMFKDGLEEFDVSRESL 191435 Query 416 NDLVSEYQQYQEA 428 D+ EY ++A Sbjct 191436 LDVTEEYLACEQA 191474 Score = 45.1 bits (105), Expect(2) = 2e-06, Method: Compositional matrix adjust. Identities = 24/80 (30%), Positives = 36/80 (45%), Gaps = 18/80 (23%) Frame = +2 Query 9 TGQCGNQIGAAFWETICGEHGLDFNGTYHGHDD------------------IQKERLNVY 50 TG + +G FW +C EHGL +G D +R +++ Sbjct 189959 TGMLLSVVGMEFWAQLCAEHGLAPDGLPLTSSD**************SPSIPLMDRKDIF 190138 Query 51 FNEASSGKWVPRSINVDLEP 70 F +A G ++PRSI +DLEP Sbjct 190139 FYQAEDGHYIPRSILIDLEP 190198 Score = 24.3 bits (51), Expect(2) = 2e-06, Method: Compositional matrix adjust. Identities = 9/13 (69%), Positives = 10/13 (77%), Gaps = 0/13 (0%) Frame = +3 Query 2 REIIHISTGQCGN 14 REII + GQCGN Sbjct 189873 REIISLQVGQCGN 189911 > scaffold-693 Length=1268102 Score = 28.5 bits (62), Expect = 7.8, Method: Compositional matrix adjust. Identities = 21/78 (27%), Positives = 36/78 (46%), Gaps = 3/78 (4%) Frame = -3 Query 337 NSDYFVEWIPNNVQTAVCSVAPQGLDMAATFIANSTSIQELFKRVGDQFSAMFKRKAFLH 396 ++D F WI N + S++ +AA NST I++L + + +F + A Sbjct 876720 STDGFPTWISLNSRKK*GSISDLMTRLAARIFFNSTWIKKLNESICCLIRPLFCKNAGSK 876541 Query 397 WYTSEG---MDELEFSEA 411 WY S ++ +FS A Sbjct 876540 WYLSVSKG*LERFKFSSA 876487 > scaffold-17 Length=2125590 Score = 28.5 bits (62), Expect = 8.0, Method: Compositional matrix adjust. Identities = 10/50 (20%), Positives = 26/50 (52%), Gaps = 0/50 (0%) Frame = +2 Query 318 RGKVSVKEVEDEMHKVQSKNSDYFVEWIPNNVQTAVCSVAPQGLDMAATF 367 + +++ ++ E+H++ + F ++IPN Q VC + G+ ++ Sbjct 477605 KNGINIGYIQQEIHRINTPLPPEFTKFIPNVKQLTVCEESHYGMKSGSSL 477754 Lambda K H a alpha 0.317 0.133 0.393 0.792 4.96 Gapped Lambda K H a alpha sigma 0.267 0.0410 0.140 1.90 42.6 43.6 Effective search space used: 2776341828 Database: X5.fasta Posted date: Nov 5, 2021 8:48 PM Number of letters in database: 23,962,143 Number of sequences in database: 1,868 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Neighboring words threshold: 13 Window for multiple hits: 40