TBLASTN 2.8.1+


Reference: Stephen F. Altschul, Thomas L. Madden, Alejandro A.
Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J.
Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of
protein database search programs", Nucleic Acids Res. 25:3389-3402.



Database: X5.fasta
           1,868 sequences; 23,962,143 total letters



Query= HXKB_YEAST P04807 Hexokinase-2 (2.7.1.1 {ECO:0000305|PubMed:332086})
(Hexokinase PII) (Hexokinase-B)

Length=486
                                                                      Score     E
Sequences producing significant alignments:                          (Bits)  Value

  scaffold-499                                                        323     4e-98
  unplaced-980                                                        318     3e-96


> scaffold-499
Length=32750

 Score = 323 bits (829),  Expect = 4e-98, Method: Compositional matrix adjust.
 Identities = 172/425 (40%), Positives = 253/425 (60%), Gaps = 19/425 (4%)
 Frame = -2

Query  34     FTVPTETLQAVTKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLR  93
              F + T  L  + KHF++EL+ GLSK G  +  +P +V   P G E+G +LA+DLGGTNLR
Sbjct  12838  FRISTIQLHEIIKHFVAELKCGLSKHGQTLKALPSFVTKLPAGMETGTYLALDLGGTNLR  12659

Query  94     VVLVKLGGDRTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQF--PQGISEPI  151
              V  V L G   F   Q KY L D  + + + D L+ +IAD  K F+DE    P    +P+
Sbjct  12658  VCEVALEGSGKFRLRQKKYVLSDEYKVS-DSDALFGYIADCTKEFLDENMIKPNADGQPL  12482

Query  152    PLGFTFSFPASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALIND  211
               LGFTFSFP     ++ GIL  W KGF++     +DVV M++    KRN+ I++VA++ND
Sbjct  12481  LLGFTFSFPVD*TSLDSGILMNWNKGFEVKGTLGNDVVAMMRDAFAKRNLNIKIVAIVND  12302

Query  212    TTGTLVASYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGK-LSDDIPPSA--------  262
              T GTLVA  Y D ET +GVI GTG N AY +   +I+K +   +  D PP+         
Sbjct  12301  TVGTLVAHCY*DGETMVGVILGTGTNAAYVESMDNIKKWESHYMKKDTPPNVRFFDDNEP  12122

Query  263    -PMAINCEYGSFDNEHVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDM  321
                M IN E+G+FDNE VVLP +KYD  +D  S  PG  T EKM SG Y+GEI+R  ++D+
Sbjct  12121  KRMVINIEWGNFDNEGVVLPISKYDRMLDAASENPGA*TLEKMVSGLYMGEIVRYIILDL  11942

Query  322    YKQGFIFKNQDL--SKFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGI---NTTVQ  376
                 G +F + ++  SKF   +   T + +RIE D  ++L DT  + ++   +   +T+V+
Sbjct  11941  ASNGHLFNSGEIEASKFMHSYSFTTLFMSRIERDHSQDLSDTKWVLEDLMCLGPDSTSVE  11762

Query  377    ERKLIRRLSELIGARAARLSVCGIAAICQKRGYKTGH-IAADGSVYNRYPGFKEKAANAL  435
              +R++++R+ E +G RAARLS   +  +  +    +G  +A DGSV+  YP FK +  +AL
Sbjct  11761  DRRIVKRVCECVGIRAARLSAACVGGVIAQMNRLSGCTVAIDGSVFEFYPHFKNRMLDAL  11582

Query  436    KDIYG  440
              ++I+G
Sbjct  11581  REIFG  11567


> unplaced-980
Length=19190

 Score = 318 bits (815),  Expect = 3e-96, Method: Compositional matrix adjust.
 Identities = 171/425 (40%), Positives = 251/425 (59%), Gaps = 19/425 (4%)
 Frame = -3

Query  34     FTVPTETLQAVTKHFISELEKGLSKKGGNIPMIPGWVMDFPTGKESGDFLAIDLGGTNLR  93
              F + T  L  + KHF++EL+ GLSK G  +  +P +V   P G E+G +LA+DLGGTNLR
Sbjct  10272  FRISTI*LHEIIKHFVAELKSGLSKHGQTLKALPSFVTKLPAGMETGTYLALDLGGTNLR  10093

Query  94     VVLVKLGGDRTFDTTQSKYRLPDAMRTTQNPDELWEFIADSLKAFIDEQF--PQGISEPI  151
              V  V L G   F     KY L D  + + + D L+ +IAD  K F+DE    P     P+
Sbjct  10092  VCEVALEGSGKFRLR*KKYVLSDEYKVS-DSDALFGYIADCTKEFLDENMIKPNADG*PL  9916

Query  152    PLGFTFSFPASQNKINEGILQRWTKGFDIPNIENHDVVPMLQKQITKRNIPIEVVALIND  211
               LGFTFSFP     ++ GIL  W KGF++     +DVV M++    KRN+ I++VA++ND
Sbjct  9915   LLGFTFSFPVD*TSLDSGILMNWNKGFEVKGTLGNDVVAMMRDAFAKRNLNIKIVAIVND  9736

Query  212    TTGTLVASYYTDPETKMGVIFGTGVNGAYYDVCSDIEKLQGK-LSDDIPPSA--------  262
              T GTLVA  Y D ET +GVI GTG N AY +   +I+K +   +  D PP+         
Sbjct  9735   TVGTLVAHCYQDGETMVGVILGTGTNAAYVESMDNIKKWESHYMKKDTPPNVRFFDDNEP  9556

Query  263    -PMAINCEYGSFDNEHVVLPRTKYDITIDEESPRPGQQTFEKMSSGYYLGEILRLALMDM  321
                M IN E+G+FDNE VVLP +KYD  +D  S  PG  T EKM SG Y+GEI+R  ++D+
Sbjct  9555   KRMVINIEWGNFDNEGVVLPISKYDRMLDAASENPGA*TLEKMVSGLYMGEIVRYIILDL  9376

Query  322    YKQGFIFKNQDL--SKFDKPFVMDTSYPARIEEDPFENLEDTDDLFQNEFGI---NTTVQ  376
                 G +F + ++  SKF   +   T + +RIE D  ++L DT  + ++   +   +T+V+
Sbjct  9375   ASNGHLFNSGEIEASKFMHSYSFTTLFMSRIERDHSQDLSDTKWVLEDLMCLGPDSTSVE  9196

Query  377    ERKLIRRLSELIGARAARLSVCGIAAICQKRGYKTG-HIAADGSVYNRYPGFKEKAANAL  435
              +R++++R+ E +G RAARLS   +  +  +    +G  +A DGSV+  YP FK +  +AL
Sbjct  9195   DRRIVKRVCECVGIRAARLSAACVGGVIAQMNRLSGCTVAIDGSVFEFYPHFKNRMLDAL  9016

Query  436    KDIYG  440
              ++I+G
Sbjct  9015   REIFG  9001



Lambda      K        H        a         alpha
   0.317    0.137    0.404    0.792     4.96 

Gapped
Lambda      K        H        a         alpha    sigma
   0.267   0.0410    0.140     1.90     42.6     43.6 

Effective search space used: 2993988480


  Database: X5.fasta
    Posted date:  Dec 26, 2021  5:01 PM
  Number of letters in database: 23,962,143
  Number of sequences in database:  1,868



Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Neighboring words threshold: 13
Window for multiple hits: 40