Running MakePangenome.. Information about input data: Number of genomes: 4 Number of sequences: 4 Number of sequences per genome: 1 Total length of input (bp): 17476994 Minimum genome length (bp): 4284494 Median genome length (bp): 4339588 Average genome length (bp): 4369248 Maximum genome length (bp): 4513324 ============================ [2020-Dec-13 19:18:27 UTC+03:00] BeginIteration 1, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 712107 Stable blocks (represented in all genomes once, but not minor): fragments: 3664 blocks: 916 Block identity: min: 0.8186 median: 0.8882 avg: 0.8888 max: 0.9947 Identity of joined blocks: 0.909379 Nucleotides in blocks: 2625892 The percentage of input length: 15.02% ============================ [2020-Dec-13 19:18:47 UTC+03:00] BeginIteration 2, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 2418997 Stable blocks (represented in all genomes once, but not minor): fragments: 12324 blocks: 3081 Block identity: min: 0.7932 median: 0.887 avg: 0.8876 max: 0.9947 Identity of joined blocks: 0.90836 Nucleotides in blocks: 8831194 The percentage of input length: 50.53% Distance from previous pre-pangenome (bp): 9223087 The percentage of input length: 52.77% Distance is sufficient to carry on ============================ [2020-Dec-13 19:19:14 UTC+03:00] BeginIteration 3, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 3213978 Stable blocks (represented in all genomes once, but not minor): fragments: 15400 blocks: 3850 Block identity: min: 0.7932 median: 0.8867 avg: 0.8875 max: 0.9947 Identity of joined blocks: 0.908116 Nucleotides in blocks: 10934219 The percentage of input length: 62.56% Distance from previous pre-pangenome (bp): 3357666 The percentage of input length: 19.21% Distance is sufficient to carry on ============================ [2020-Dec-13 19:19:42 UTC+03:00] BeginIteration 4, AnchorJoinerFast Length of all major blocks of at least 2 fragments: 3945898 Stable blocks (represented in all genomes once, but not minor): fragments: 18592 blocks: 4648 Block identity: min: 0.7932 median: 0.8877 avg: 0.8882 max: 0.9947 Identity of joined blocks: 0.908297 Nucleotides in blocks: 13490688 The percentage of input length: 77.19% Distance from previous pre-pangenome (bp): 2788020 The percentage of input length: 15.95% Distance is sufficient to carry on ============================ [2020-Dec-13 19:20:14 UTC+03:00] BeginIteration 5, AnchorJoiner Length of all major blocks of at least 2 fragments: 4137654 Stable blocks (represented in all genomes once, but not minor): fragments: 18824 blocks: 4706 Block identity: min: 0.7932 median: 0.8878 avg: 0.8882 max: 0.9947 Identity of joined blocks: 0.908275 Nucleotides in blocks: 13668068 The percentage of input length: 78.2% Distance from previous pre-pangenome (bp): 495872 The percentage of input length: 2.84% Distance is sufficient to carry on ============================ [2020-Dec-13 19:21:49 UTC+03:00] BeginIteration 6, AnchorJoiner Length of all major blocks of at least 2 fragments: 4136027 Stable blocks (represented in all genomes once, but not minor): fragments: 18836 blocks: 4709 Block identity: min: 0.7932 median: 0.8877 avg: 0.8882 max: 0.9947 Identity of joined blocks: 0.908273 Nucleotides in blocks: 13668507 The percentage of input length: 78.2% Distance from previous pre-pangenome (bp): 32244 The percentage of input length: 0.18% Distance is sufficient to carry on ============================ [2020-Dec-13 19:22:35 UTC+03:00] BeginIteration 7, AnchorJoiner Length of all major blocks of at least 2 fragments: 4136108 Stable blocks (represented in all genomes once, but not minor): fragments: 18844 blocks: 4711 Block identity: min: 0.7932 median: 0.8877 avg: 0.8881 max: 0.9947 Identity of joined blocks: 0.908267 Nucleotides in blocks: 13669734 The percentage of input length: 78.21% Distance from previous pre-pangenome (bp): 5192 The percentage of input length: 0.03% Distance is too low => stopping this loop ============================ [2020-Dec-13 19:23:07 UTC+03:00] BeginIteration 8, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 4132739 Stable blocks (represented in all genomes once, but not minor): fragments: 19048 blocks: 4762 Block identity: min: 0.7932 median: 0.8873 avg: 0.8878 max: 0.9947 Identity of joined blocks: 0.908144 Nucleotides in blocks: 13685785 The percentage of input length: 78.3% Distance from previous pre-pangenome (bp): 142154 The percentage of input length: 0.81% Distance is sufficient to carry on ============================ [2020-Dec-13 19:24:16 UTC+03:00] BeginIteration 9, AnchorBlastJoiner Length of all major blocks of at least 2 fragments: 4133587 Stable blocks (represented in all genomes once, but not minor): fragments: 19040 blocks: 4760 Block identity: min: 0.7932 median: 0.8873 avg: 0.8878 max: 0.9947 Identity of joined blocks: 0.908137 Nucleotides in blocks: 13687343 The percentage of input length: 78.31% Distance from previous pre-pangenome (bp): 4982 The percentage of input length: 0.03% Distance is too low => stopping this loop ============================ [2020-Dec-13 19:26:11 UTC+03:00] StopInfo The pangenome has been constructed. Blocks' lengths: 5439736 major: 5401213 minor: 38523 ============================ All major blocks of at least 2 fragments: fragments: 12087 blocks: 3361 fragments / blocks: 3.5962 Block identity: min: 0.7233 median: 0.9142 avg: 0.9242 max: 1 Identity of joined blocks: 0.912067 Block sizes: min: 2 median: 4 avg: 3.60 max: 21 Fragment lengths: min: 50 median: 364 avg: 1342.61 max: 29994 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.005 max: 0.6973 GC content: min: 0.3447 median: 0.6144 avg: 0.6085 max: 0.8144 Nucleotides in blocks: 16228126 The percentage of input length: 92.85% Total length of blocks: 4229474 The percentage of total blocks' length: 77.75% ============================ Stable blocks (represented in all genomes once, but not minor): fragments: 6564 blocks: 1641 fragments / blocks: 4 Block identity: min: 0.7485 median: 0.8843 avg: 0.8836 max: 0.9937 Identity of joined blocks: 0.899612 Block sizes: 4 Fragment lengths: min: 87 median: 1143 avg: 2138.23 max: 25171 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0.0008 avg: 0.004 max: 0.2282 GC content: min: 0.4044 median: 0.6192 avg: 0.6156 max: 0.7554 Nucleotides in blocks: 14035360 The percentage of input length: 80.3% Total length of blocks: 3522617 The percentage of total blocks' length: 64.75% ============================ Partial blocks (represented once in subset of genomes): fragments: 4290 blocks: 1484 fragments / blocks: 2.8908 Block identity: min: 0.7594 median: 0.9907 avg: 0.9759 max: 1 Identity of joined blocks: 0.980122 Block sizes: min: 2 median: 3 avg: 2.89 max: 3 Fragment lengths: min: 82 median: 180 avg: 429.84 max: 29994 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0041 max: 0.3963 GC content: min: 0.3447 median: 0.6019 avg: 0.6001 max: 0.8144 Nucleotides in blocks: 1844030 The percentage of input length: 10.55% Total length of blocks: 643411 The percentage of total blocks' length: 11.82% ============================ Blocks with repeats (at least two copies in at least one genome): fragments: 1233 blocks: 236 fragments / blocks: 5.2245 Block identity: min: 0.7233 median: 0.8633 avg: 0.8808 max: 1 Identity of joined blocks: 0.91343 Block sizes: min: 2 median: 5 avg: 5.22 max: 21 Fragment lengths: min: 50 median: 128 avg: 282.84 max: 2800 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0173 max: 0.6973 GC content: min: 0.4517 median: 0.6174 avg: 0.6119 max: 0.7411 Nucleotides in blocks: 348736 The percentage of input length: 1.99% Total length of blocks: 63446 The percentage of total blocks' length: 1.16% ============================ Unique (blocks of 1 fragment but not minor): fragments: 1567 blocks: 1567 Fragment lengths: min: 100 median: 193 avg: 747.76 max: 55246 GC content: min: 0.379 median: 0.6024 avg: 0.6012 max: 0.8086 Nucleotides in blocks: 1171739 The percentage of input length: 6.7% Total length of blocks: 1171739 The percentage of total blocks' length: 21.54% ============================ Minor blocks (too short to say smth about): fragments: 2549 blocks: 1106 fragments / blocks: 2.3047 Block identity: min: 0 median: 1 avg: 0.7582 max: 1 Identity of joined blocks: 0.937957 Block sizes: min: 1 median: 3 avg: 2.30 max: 14 Fragment lengths: min: 1 median: 14 avg: 30.26 max: 99 Fragment length spreading ((max - min) / avg) inside block: min: 0 median: 0 avg: 0.0354 max: 2.4444 GC content: min: 0 median: 0.6011 avg: 0.5843 max: 1 Nucleotides in blocks: 77129 The percentage of input length: 0.44% Total length of blocks: 38523 The percentage of total blocks' length: 0.7% The pangenome has been prepared for the next step: PostProcessing .. done